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A Short Extraction Time of Polysaccharides from Fenugreek (Trigonella foencem graecum) Seed Using Continuous Ultrasound Acoustic Cavitation: Process Optimization, Characterization and Biological Activities

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Abstract

In this study, the effective variables involved in ultrasound-assisted extraction (UAE) of fenugreek (Trigonella foenum graecum) seeds polysaccharides (FSPs) were optimized through Box–Behnken response surface design. The maximum yield of FSPs was 33.49% under the modified optimum conditions of an ultrasound power of 120 W, an irradiation time of 22 min and a liquid-to-solid ratio (L/S) of 30:1 mL g−1. The predicted yield was in a very good agreement with the experimental yield of 33.41%. This value was higher than the FSPs yield obtained using conventional extraction (CE) method for 180 min at the same extraction temperature and L/S ratio. The primary chemical and structural characteristics were investigated by UV, FT-IR, and GC-MS. FSPs found to be a heteropolysaccharide consisted of galactose (38.18%), glucose (3.71%), mannose (46.13%), rhamnose (1.02%), and arabinose (0.83%). Furthermore, the FSPs exhibited considerable scavenging activity against 1,1-Diphenyl-2-Picrylhydrazyl free radicals and ferric reducing antioxidant power and reducing power, in a concentration-dependent mode in vitro. Our results suggested that UAE technique gave a higher yield of FSPs with a shorter extraction time than the CE and FSPs can also be used as promising resources of natural agent for functional foods and medicinal industries.

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References

  • Ahmadiani, A., Javan, M., Semnanian, S., Barat, E., & Kamalinejad, M. (2001). Anti-inflammatory and antipyretic effects of Trigonella foenum-graecum leaves extract in the rat. Journal of Ethnopharmacology, 75(2-3), 283–286.

    Article  CAS  Google Scholar 

  • Alzorqi, I., Singh, A., Manickam, S., & Al-Qrimli, H. F. (2017). Optimization of ultrasound assisted extraction (UAE) of β-D-glucan polysaccharides from Ganoderma lucidum for prospective scale-up. Resource-Efficient Technologies, 3(1), 46–54.

    Article  Google Scholar 

  • Amin, A., Alkaabi, A., Al-Falasi, S., & Daoud, S. A. (2005). Chemopreventive activities of Trigonella foenum-graecum (fenugreek) against breast cancer. Cell Biology International, 29(8), 687–694.

    Article  Google Scholar 

  • AOAC. (1990). Association of official analytical chemists (13 th ed.). Washington, DC, USA.

  • Benzie, I. F. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The frap assay. Analytical Biochemistry, 239(1), 70–76.

    Article  CAS  Google Scholar 

  • Brummer, Y., Cui, W., & Wang, Q. (2003). Extraction, purification and physicochemical characterization of fenugreek gum. Food Hydrocolloids, 17(3), 229–236.

    Article  CAS  Google Scholar 

  • Chen, R., Li, Y., Dong, H., Liu, Z., Li, S., Yang, S., & Li, X. (2012). Optimization of ultrasonic extraction process of polysaccharides from Ornithogalum Caudatum Ait and evaluation of its biological activities. Ultrasonics Sonochemistry, 19(6), 1160–1168.

    Article  CAS  Google Scholar 

  • Cheung, Y.-C., Siu, K.-C., & Wu, J.-Y. (2013). Kinetic models for ultrasound-assisted extraction of water-soluble components and polysaccharides from medicinal fungi. Food and Bioprocess Technology, 6(10), 2659–2665.

    Article  CAS  Google Scholar 

  • Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28(3), 350–356.

    Article  CAS  Google Scholar 

  • Duh, P. D., Du, P. C., & Yen, G. C. (1999). Action of methanolic extract of mung bean hulls as inhibitors of lipid peroxidation and non-lipid oxidative damage. Food and Chemical Toxicology, 37(11), 1055–1061.

    Article  CAS  Google Scholar 

  • Fathi, M., Mohebbi, M., & Koocheki, A. (2016). Some physico-chemical properties of Prunus armeniaca L. gum exudates. International Journal of Biological Macromolecules, 82, 744–750.

    Article  CAS  Google Scholar 

  • Feng, K., Chen, W., Sun, L., Liu, J., Zhao, Y., Li, L., Wang, Y., & Zhang, W. (2015). Optimization extraction, preliminary characterization and antioxidant activity in vitro of polysaccharides from Stachys sieboldii Miq. Tubers. Carbohydrate Polymers, 125, 45–52.

    Article  CAS  Google Scholar 

  • Feng, S., Cheng, H., Fu, L., Ding, C., Zhang, L., Yang, R., & Zhou, Y. (2014). Ultrasonic-assisted extraction and antioxidant activities of polysaccharides from Camellia oleifera leaves. International Journal of Biological Macromolecules, 68, 7–12.

    Article  CAS  Google Scholar 

  • Gharibzahedi, S. M. T., Mousavi, S. M., Khodaiyan, F., & Hamedi, M. (2012). Optimization and characterization of walnut beverage emulsions in relation to their composition and structure. International Journal of Biological Macromolecules, 50(2), 376–384.

    Article  CAS  Google Scholar 

  • Han, L., Suo, Y., Yang, Y., Meng, J., & Hu, N. (2016). Optimization, characterization, and biological activity of polysaccharides from Berberis dasystachya maxim. International Journal of Biological Macromolecules, 85, 655–666.

    Article  CAS  Google Scholar 

  • Hromádková, Z., Ebringerová, A., & Valachovic, P. (2002). Ultrasound-assisted extraction of water-soluble polysaccharides from the roots of valerian (Valeriana officinalis L.). Ultrasonics Sonochemistry, 9(1), 37–44.

    Article  Google Scholar 

  • Hu, J., Jia, X., Fang, X., Li, P., He, C., & Chen, M. (2016). Ultrasonic extraction, antioxidant and anticancer activities of novel polysaccharides from chuanxiong rhizome. International Journal of Biological Macromolecules, 85, 277–284.

    Article  CAS  Google Scholar 

  • Hung, P. V., & Nhi, N. N. Y. (2012). Nutritional composition and antioxidant capacity of several edible mushrooms grown in the southern Vietnam. International Food Research Journal, 19(2), 611–615.

    CAS  Google Scholar 

  • Jalili Safaryan, M., Ganjloo, A., Bimakr, M., & Zarringhalami, S. (2016). Optimization of ultrasound-assisted extraction, preliminary characterization and in vitro antioxidant activity of polysaccharides from green pea pods. Foods, 5(4), 78. https://doi.org/10.3390/foods5040078.

    Article  CAS  PubMed Central  Google Scholar 

  • Jia, X. J., Ding, C. B., Yuan, S., Zhang, Z. W., Du, L., & Yuan, M. (2014). Extraction, purification and characterization of polysaccharides from hawk tea. Carbohydrate Polymers, 99, 319–324.

    Article  CAS  Google Scholar 

  • Jiang, J. X., Zhu, L. W., Zhang, W. M., & Sun, R. C. (2007). Characterization of galactomannan gum from fenugreek (Trigonella foenum-graecum) seeds and its rheological properties. International Journal of Polymeric Materials, 56(12), 1145–1154.

    Article  CAS  Google Scholar 

  • Jooyandeh, H., Noshad, M., & Amir Khamirian, R. (2018). Modeling of ultrasound-assisted extraction, characterization and in vitro pharmacological potential of polysaccharides from Vaccinium arctostaphylos L. International Journal of Biological Macromolecules, 107(Pt A), 938–948.

    Article  CAS  Google Scholar 

  • Kannan, S., Manivannan, R., Balasubramaniam, A., & Kumar, N. S. (2010). Formulation and evaluation of aspirin delayed release tablet. International Journal of Comprehensive Pharmacy, 1–3.

  • Kaviarasan, S., Vijayalakshmi, K., & Anuradha, C. V. (2004). Polyphenol-rich extract of fenugreek seeds protect erythrocytes from oxidative damage. Plant Foods for Human Nutrition, 59(4), 143–147.

    Article  CAS  Google Scholar 

  • Ktari, N., Trabelsi, I., Bardaa, S., Triki, M., Bkhairia, I., Ben Slama-Ben Salem, R., Nasri, M., & Ben Salah, R. (2017). Antioxidant and hemolytic activities, and effects in rat cutaneous wound healing of a novel polysaccharide from fenugreek (Trigonella foenum-graecum) seeds. International Journal of Biological Macromolecules, 95, 625–634.

    Article  CAS  Google Scholar 

  • Lai, F., Wen, Q., Li, L., Wu, H., & Li, X. (2010). Antioxidant activities of water-soluble polysaccharide extracted from mung bean (Vignaradiata L.) hull with ultrasonic assisted treatment. Carbohydrate Polymers, 81(2), 323–329.

    Article  CAS  Google Scholar 

  • Leela, N. K., & Shafeekh, K. M. (2008). Fenugreek. In Chemistry of spices, Ed. By Parthasarathy V.A., Chempakam B., Zachariah T.J. CAB International.

  • Li, L., Ding, C., Yuan, S., Zhang, Z., Chen, Y., Hu, C., et al. (2016). Ultrasonic-assisted enzymatic extraction and antioxidant activity of polysaccharides from Setaria viridis. Separation Science and Technology, 51(11), 1798–1805.

    Article  CAS  Google Scholar 

  • Li, Q., Yu, N., Wang, Y., Sun, Y., Lu, K., & Guan, W. (2013). Extraction optimization of Bruguiera gymnorrhiza polysaccharides with radical scavenging activities. Carbohydrate Polymers, 96(1), 148–155.

    Article  CAS  Google Scholar 

  • Liu, D.-S., Yan, L., & Han, J.-T. (2012). Extraction, identification and content determination of polysaccharides from the leaves of Prunus pseudocerasus Lindl. Journal of Anhui Agricultural Sciences, 40, 10861–10862.

    CAS  Google Scholar 

  • Liu, J., Willför, S., & Xu, C. (2015). A review of bioactive plant polysaccharides: Biological activities, functionalization, and biomedical applications. Bioactive Carbohydrates and Dietary Fibre, 5(1), 31–61.

    Article  CAS  Google Scholar 

  • Liu, X. L., Qiu, H. Y., Wang, Q., Wu, L. Y., & Zhang, C. H. (2011). Qualitative study on chemical constituents of Amomum tsao-ko. China Condiment, 36(1), 104–106.

    Google Scholar 

  • Ma, C.-w., Feng, M., Zhai, X., Hu, M., You, L., Luo, W., & Zhao, M. (2013). Optimization for the extraction of polysaccharides from Ganoderma lucidum and their antioxidant and antiproliferative activities. Journal of the Taiwan Institute of Chemical Engineers, 44(6), 886–894.

    Article  CAS  Google Scholar 

  • Madar, Z., & Shomer, I. (1990). Polysaccharide composition of a gel fraction derived from fenugreek and its effect on starch digestion and bile acid absorption in rats. Journal of Agricultural and Food Chemistry, 38(7), 1535–1539.

    Article  CAS  Google Scholar 

  • Myers, R. H., Montgomery, D. C., & Anderson-Cook, C. M. (2016). Response surface methodology: Process and product optimization using designed experiments. John Wiley & Sons, Hoboken, New Jersey, USA, 4th edition.

  • Noshad, M., Mohebbi, M., Shahidi, F., & Mortazavi, S. A. (2012). Multi-objective optimization of osmotic–ultrasonic pretreatments and hot-air drying of quince using response surface methodology. Food and Bioprocess Technology, 5(6), 2098–2110.

    Article  Google Scholar 

  • Ochoa-Rivas, A., Nava-Valdez, Y., Serna-Saldívar, S. O., & Chuck-Hernández, C. (2017). Microwave and ultrasound to enhance protein extraction from peanut flour under alkaline conditions: Effects in yield and functional properties of protein isolates. Food and Bioprocess Technology, 10(3), 543–555.

    Article  CAS  Google Scholar 

  • Oyaizu, M. (1986). Studies on product of browning reaction prepared from glucose amine. The Japanese Journal of Nutrition and Dietetics, 44(6), 307–315.

    Article  CAS  Google Scholar 

  • Pakrokh Ghavi, P. (2015). The extraction process optimization of antioxidant polysaccharides from marshmallow (Althaea officinalis L.) roots. International Journal of Biological Macromolecules, 75, 51–57.

    Article  CAS  Google Scholar 

  • Raza, A., Li, F., Xu, X., & Tang, J. (2017). Optimization of ultrasonic-assisted extraction of antioxidant polysaccharides from the stem of Trapa quadrispinosa using response surface methodology. International Journal of Biological Macromolecules, 94(Pt A), 335–344.

    Article  CAS  Google Scholar 

  • Razavi, S. M. A., Taheri, H., & Quinchia, L. A. (2011). Steady shear flow properties of wild sage (Salvia macrosiphon) seed gum as a function of concentration and temperature. Food Hydrocolloids, 25(3), 451–458.

    Article  CAS  Google Scholar 

  • Sarfarazi, M., Jafari, S. M., & Rajabzadeh, G. (2015). Extraction optimization of saffron nutraceuticals through response surface methodology. Food Analytical Methods, 8(9), 2273–2285.

    Article  Google Scholar 

  • Sevag, M. G., Lackman, D. B., & Smolens, J. (1938). The isolation of the components of streptococcal nucleoproteins in serologically active form. Journal of Biological Chemistry, 124(2), 425–436.

    CAS  Google Scholar 

  • Song, M., Li, Q. X., & He, C. L. (2009). Identification and analgesic effect of Angelica sinensis extract. Journal of Xiang Ning University, 23, 194–196.

    Google Scholar 

  • Srinivasan, K. (2006). Fenugreek (Trigonella foenum-graecum): A review of health beneficial physiological effects. Food Reviews International, 22(2), 203–224.

    Article  CAS  Google Scholar 

  • Sun, R. C., & Tomkinsin, J. (2002). Comparative study of lignins isolated by alkali and ultrasound-assisted alkali extractions from wheat straw. Ultrasonics Sonochemistry, 9(2), 85–93.

    Article  CAS  Google Scholar 

  • Taylor, W. G., Zaman, M. S., Mir, Z., Mir, P. S., Acharya, S. N., Mears, G. J., & Elder, J. L. (1997). Analysis of steroidal sapogenins from amber fenugreek (Trigonella foenum-graecum) by capillary gas chromatography and combined gas chromatography/mass spectrometry. Journal of Agriculture and Food Chemistry, 45(3), 753–759.

    Article  CAS  Google Scholar 

  • Tian, Y., Zheng, B., Chen, C., & Zheng, Y. (2012a). Ultrasound-assisted extraction, preliminary characterization, and antioxidant activity of a novel water-soluble polysaccharide from Lotus (Nelumbo nucifera Gaertn.) seeds. Separation Science and Technology, 47(16), 2408–2416.

    CAS  Google Scholar 

  • Tian, Y., Zeng, H., Xu, Z., Zheng, B., Lin, Y., Gan, C., & Lo, Y. M. (2012b). Ultrasonic-assisted extraction and antioxidant activity of polysaccharides recovered from white button mushroom (Agaricus bisporus). Carbohydrate Polymers, 88(2), 522–529.

    Article  CAS  Google Scholar 

  • Vodenicarová, M., Drímalová, G., Hromádková, Z., Malovíková, A., & Ebringerová, A. (2006). Xyloglucan degradation using different radiation sources: A comparative study. Ultrasonics Sonochemistry, 13(2), 157–164.

    Article  Google Scholar 

  • Wang, J., Zhang, Q. B., Zhang, Z. S., & Li, Z. (2008). Antioxidant activity of sulfated polysaccharide fractions extracted from Laminaria japonica. International Journal of Biological Macromolecules, 42(2), 127–132.

    Article  Google Scholar 

  • Wang, Q., Sun, Y., Yang, B., Wang, Z., Liu, Y., Qi Cao, Q., Sun, X., & Kuang, H. (2014). Optimization of polysaccharides extraction from seeds of Pharbitis nil and its anti-oxidant activity. Carbohydrate Polymers, 102, 460–466.

    Article  CAS  Google Scholar 

  • Xie, J.-H., Shen, M.-Y., Xie, M.-Y., Nie, S.-P., Chen, Y., Li, C., Huang, D. F., & Wang, Y. X. (2012). Ultrasonic-assisted extraction, antimicrobial and antioxidant activities of Cyclocarya paliurus (batal.) iljinskaja polysaccharides. Carbohydrate Polymers, 89(1), 177–184.

    Article  CAS  Google Scholar 

  • Yang, N., Jin, Y., Jin, Z., & Xu, X. (2016). Electric-field-assisted extraction of garlic polysaccharides via experimental transformer device. Food and Bioprocess Technology, 9(9), 1612–1622.

    Article  CAS  Google Scholar 

  • Ying, Z., Han, X., & Li, J. (2011). Ultrasound-assisted extraction of polysaccharides from mulberry leaves. Food Chemistry, 127(3), 1273–1279.

    Article  CAS  Google Scholar 

  • Yu, P., & Zhang, Y. (2017). Separation and purification of Porphyra haitanensis polysaccharide and its preliminary structural characterization. Separation Science and Technology, 52(11), 1835–1842.

    Article  CAS  Google Scholar 

  • Zhang, D.-Y., Wan, Y., Xu, J.-Y., Wu, G.-H., Li, L., & Yao, X.-H. (2016). Ultrasound extraction of polysaccharides from mulberry leaves and their effect on enhancing antioxidant activity. Carbohydrate Polymers, 137, 473–479.

    Article  CAS  Google Scholar 

  • Zhang, L., & Wang, M. (2017). Optimization of deep eutectic solvent-based ultrasound-assisted extraction of polysaccharides from Dioscorea opposita Thunb. International Journal of Biological Macromolecules, 95, 675–681.

    Article  CAS  Google Scholar 

  • Zhang, Z., Lv, G., He, W., Shi, L., Pan, H., & Fan, L. (2013). Effects of extraction methods on the antioxidant activities of polysaccharides obtained from Flammulina velutipes. Carbohydrate Polymers, 98(2), 1524–1531.

    Article  CAS  Google Scholar 

  • Zhang, Y., Zhang, H., Wang, L., Qian, H., & Qi, X. (2015). Extraction of oat (Avena sativa L.) antifreeze proteins and evaluation of their effects on frozen dough and steamed bread. Food and Bioprocess Technology, 8(10), 2066–2075.

    Article  CAS  Google Scholar 

  • Zhao, Q., Kennedy, J. F., Wang, X., Yuan, X., Zhao, B., Peng, Y., & Huang, Y. (2011). Optimization of ultrasonic circulating extraction of polysaccharides from Asparagus officinalis using response surface methodology. International Journal of Biological Macromolecules, 49(2), 181–187.

    Article  CAS  Google Scholar 

  • Zhao, T. T., Zhang, Q. B., Qi, H. M., Zhang, H., Niu, X., Xu, Z. H., et al. (2006). Degradation of porphyran from porphyra haitanensis and the antioxidant activities of the degraded porphyrans with different molecular weight. International Journal of Biological Macromolecules, 38(1), 45–50.

    Article  Google Scholar 

  • Zheng, Q., Ren, D., Yang, N., & Yang, X. (2016). Optimization for ultrasound-assisted extraction of polysaccharides with chemical composition and antioxidant activity from the Artemisia sphaerocephala Krasch seeds. International Journal of Biological Macromolecules, 91, 856–866.

    Article  CAS  Google Scholar 

  • Zhu, J., Yang, Y., Wen, Y., & Zhao, G. (2013). An improved ultrasound-assisted alkali extraction process of Perilla seed meal polysaccharide. Separation Science and Technology, 48(18), 2771–2778.

    Article  CAS  Google Scholar 

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  1. Department of Food Science and Engineering, Faculty of Agriculture, University of Zanjan, Zanjan, Iran

    Ala Ghasemi Kia, Ali Ganjloo & Mandana Bimakr

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  1. Ala Ghasemi Kia
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  2. Ali Ganjloo
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  3. Mandana Bimakr
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Correspondence to Ali Ganjloo.

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Kia, A.G., Ganjloo, A. & Bimakr, M. A Short Extraction Time of Polysaccharides from Fenugreek (Trigonella foencem graecum) Seed Using Continuous Ultrasound Acoustic Cavitation: Process Optimization, Characterization and Biological Activities. Food Bioprocess Technol 11, 2204–2216 (2018). https://doi.org/10.1007/s11947-018-2178-2

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