Skip to main content
SpringerLink
Log in

Polyphenol-Rich Extract of Fenugreek Seeds Protect Erythrocytes from Oxidative Damage

  • Published:
Plant Foods for Human Nutrition Aims and scope Submit manuscript

Abstract

A polyphenol-rich extract from the seeds of fenugreek was evaluated for its protective effect against hydrogen peroxide(H2O2)-induced oxidation in normal and diabetic human erythrocytes (RBCs). RBCs, preincubated with increasing amounts of fenugreek seed extract and challenged with H2O2, were analyzed for hemolysis and lipid peroxidation. RBCs from diabetic subjects were more susceptible to oxidative hemolysis and lipid peroxidation than those from normal subjects. However preincubation with the polyphenol-rich extract significantly reduced the oxidative modifications in both the groups. The inhibition of lipid peroxidation was concentration-dependent up to 100 μl of extract, which contained 0.75mM gallic acid equivalent (GAE) of phenolic compounds. These findings demonstrate the potent antioxidant properties of the fenugreek seeds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. West IC (2000) Radicals and oxidative stress in diabetes. Diabet Med 17: 171–180.

    Google Scholar 

  2. Baynes JW, Thorpe SR (1999) Role of oxidative stress in diabetic complication: A new perspective on an old paradigm. Diabetes 48: 1–9.

    Google Scholar 

  3. Santini SA, Marra G, Giardina B, Cotroneo P, Mordente A, Martorana GE, et al. (1997) Defective plasma antioxidant defences and enhanced susceptibility to lipid peroxidation in uncomplicated IDDM. Diabetes 46: 1853–1858.

    Google Scholar 

  4. Bursell SE, Clermont AC, Aiello IP, Aiello IM, Schlossman DK, Feener FP, Laffel LI, King GI (1999) High -dose vitamin E supplementation normalizes retinal flow and creatinine clearance in patients with type I diabetes. Diabetes Care 22: 1245–1251.

    Google Scholar 

  5. Maridonneau I, Braquet P, Garay RP (1983) Na + and K+ transport damage induced by oxygen free radicals in human red cell membrane. J Biol Chem 258: 3107–3113.

    Google Scholar 

  6. Rohn TT, Nelson LK, Waeg G, Quinn MT (1998) U -101033E (2,4-diaminopyrrolopyrimidine), a potent inhibitor of membrane lipid peroxidation as assessed by the production of 4-hydroxynonenal, malondialdehyde and 4-hydroxynonenal-protein adducts. Biochem Pharmacol 56: 1371–1379.

    Google Scholar 

  7. Jain SK, Levine SN, Duett J, Hollier B (1990) Elevated lipid peroxidation levels in red blood cells of streptozotocin-treated diabetic rats. Metabolism 39: 971–975.

    Google Scholar 

  8. Jain SK, Levine SN, Duett J, Hollier B (1991) Reduced vitamin E and increased lipofuscin products in erythrocytes of diabetic rats. Diabetes 40: 1241–1244.

    Google Scholar 

  9. Schwartz RS, Madsen JW, Rybicki AC, Nagel RL (1991) Oxidation of spectrin and deformability defects in diabetic erythrocytes. Diabetes 40: 701–708.

    Google Scholar 

  10. Middleton JRE, Kandaswami C, Theoharides CT (2000) The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease and cancer. Pharmacol Rev 52(4): 673–751.

    Google Scholar 

  11. Khosla P, Gupta DD, Nagpal KK (1995) Effect of Trigonella foenum graecum (Fenugreek) on blood glucose in normal and diabetic rats. Indian J Physiol Pharmacol 39: 173–174.

    Google Scholar 

  12. Ribes G, Sauvaire Y, Costa CD, Baccou JC, Loubatieres-Mariani MM (1986) Antidiabetic effects of subfraction from fenugreek seeds in diabetic dogs. Proc Soc Exp Biol Med 182: 159–166.

    Google Scholar 

  13. Gupta A, Gupta R, Lal B (2001) Effect of Trigonella foenum graecum (fenugreek) seeds on glycaemic control and insulin resistance in type 2 diabetes mellitus: A double blind placebo controlled study. J Assoc Physicians India 49: 1057–1061.

    Google Scholar 

  14. Sharma RD (1986) An evaluation of hypocholesterolemic factor of fenugreek seeds in rats. Nutr Rep Int 33: 669–677.

    Google Scholar 

  15. Sur P, Das M, Gomes A, Vedasiromoni JR, Sahu NP, Banerjee S, Sharma S, Ganguly DK (2001) Trigonella foenum graecum (Fenugreek) seed extract as an antineoplastic agent. Phytother Res 15(3): 257–259.

    Google Scholar 

  16. Anuradha CV, Ravikumar P (2001) Restoration of tissue antioxidants by fenugreek seeds (Trigonella foenum graecum) in alloxan-diabetic rats. Indian J Physiol Pharmacol 45: 408–420.

    Google Scholar 

  17. Xia J, Allenbrand B, Sun GY (1998) Dietary supplementation of grape polyphenols and chronic ethanol administration on LDL oxidation and platelet function in rats. Life Sci 63: 383–390.

    Google Scholar 

  18. Singleton SL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16: 144–158.

    Google Scholar 

  19. Grinberg LN, Newmark H, Kitrossky N, Rahamim E, Chevion M, Rachmilewitz EA (1997) Protective effect of tea polyphenols against oxidative damage to red blood cells. Biochem Pharmacol 54: 973–978.

    Google Scholar 

  20. Tedesco I, Russo M, Russo P, Iacomino G, Russo GL, Carraturo A, Clementina F, Moio L, Palumbo R (2000) Antioxidant effect of red wine polyphenols on red blood cells. J Nutr Biochem 11: 114–119.

    Google Scholar 

  21. Niehaus WG, Samuelsson B (1968) Formation of MDA from phospholipid arachidonate during microsomal lipid peroxidation. Eur J Biochem 6: 126–130.

    Google Scholar 

  22. Drabkin DL, Austin JM (1932) Spectrophotometric studies spectrophotometric constants for common haemoglobin derivatives in human, dog, and rabbits blood. J Biol Chem 98: 719–739.

    Google Scholar 

  23. Stocks J, Dormandy TL (1971) The autoxidation of human red cell lipids induced by hydrogen peroxide. Br J Hematol 20: 95–111.

    Google Scholar 

  24. Puppo A, Halliwell B (1988) Formation of hydroxyl radicals from hydrogen peroxide in the presence of iron. Is hemoglobin a biological fenton catalyst? Biochem J 249: 185–190.

    Google Scholar 

  25. Wiseman H (1996) Dietary influences on membrane function: Importance in protection against oxidative damage and disease. J Nutr Biochem 7: 2–15.

    Google Scholar 

  26. Ravikumar P, Anuradha CV (1999) Effect of fenugreek on blood lipid peroxidation and antioxidants in diabetic rats. Phytother Res 13: 1–5.

    Google Scholar 

  27. Anuradha CV, Ravikumar P (1998) Anti -lipid peroxidative activity of seeds of fenugreek (Trigonella foenum graecum). Med Sci Res 26: 317–321.

    Google Scholar 

  28. Gupta R, Nair S (1999) Antioxidant flavonoids in common Indian diet. South Asian J Prev Cardiol 3: 83–94.

    Google Scholar 

  29. Shang M, Cais, HJ, Li J, Zhao Y, Zheng J, Namba T, Kadota S, Tezuka Y, Fan W (1998) Studies on flavonoids from fenugreek (Trigonella foenum graecum L). Zhongguo Zhong Yao Za Zhi 23(10): 614–639.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, Annamalai University, Annamalainagar, 608 002, Tamil Nadu, India

    S. KAVIARASAN, K. VIJAYALAKSHMI & C.V. ANURADHA

Authors
  1. S. KAVIARASAN
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. VIJAYALAKSHMI
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C.V. ANURADHA
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to C.V. ANURADHA.

Rights and permissions

Reprints and permissions

About this article

Cite this article

KAVIARASAN, S., VIJAYALAKSHMI, K. & ANURADHA, C. Polyphenol-Rich Extract of Fenugreek Seeds Protect Erythrocytes from Oxidative Damage. Plant Foods Hum Nutr 59, 143–147 (2004). https://doi.org/10.1007/s11130-004-0025-2

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11130-004-0025-2

Search

Navigation