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Modeling and Optimization of Lactic Acid Production using Cashew Apple Juice as Substrate

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Abstract

The production of lactic acid by Lactobacillus casei B-442 was studied and modeled. Sugar feedstock was provided using cashew apple juice, an alternative glucose and fructose feedstock that proved to yield high concentrations of lactic acid. The fermentations were carried out in a 1-L fermenter under constant agitation (150 rpm) and controlled pH (6.5). Lactic acid production was evaluated through a dynamic study, varying the initial concentration of sugar in the range of 20 to 60 g/L. Biomass, reducing sugars, and lactic acid concentration were measured throughout the experiments. The highest production of lactic acid (59.3 g/L) was obtained operating the fermentation with 60 g/L of reducing sugar from the cashew apple juice. A rigorous kinetic model was developed for batch fermentation of cashew apple juice for lactic acid production by L. casei B-442. The growth of biomass and lactic acid production were affected by substrate limitation, substrate inhibition and lactic acid inhibition. The model assumed growth- and non-growth-associated lactic acid production and a term for microorganism death was also included in the model. Parameters of the kinetic model were determined based on experimental data by using the least mean squares method and Levenberg–Marquardt algorithm. The model validation was performed and the model was statistically able to fit the profiles for growth of biomass, sugar consumption and lactic acid production. The optimization of the process, using the model, was carried out and the optimum operating conditions aiming highest productivity, lowest production cost and highest gross profit are presented.

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References

  • Akerberg, C., & Zacchi, G. (2000). An economic evaluation of the fermentative production of lactic acid from wheat flour. Bioresource Technology, 75, 119–126.

    Article  CAS  Google Scholar 

  • Ali, Z., Anjum, F. M., & Zahoor, T. (2009). Production of lactic acid from corn cobs hydrolysate through fermentation by Lactobaccillus delbrukii. African Journal of Biotechnology, 8, 4175–4178.

    CAS  Google Scholar 

  • Altaf, Md, Naveena, B. J., & Reddy, G. (2007). Use of inexpensive nitrogen sources and starch for l(+) lactic acid production in anaerobic submerged fermentation. Bioresource Technology, 98, 498–503.

    Article  CAS  Google Scholar 

  • Bouguettoucha, A., Nacef, S., Balannec, B., & Amrane, A. (2009). Unstructured models for growth and lactic acid production during two-stage continuous cultures of Lactobacillus helveticus. Process Biochemistry, 44, 742–748.

    Article  CAS  Google Scholar 

  • Cui, F., Wan, C., Li, Y., Liu, Z., & Rajashekara, G. (2010). Co-production of lactic acid and Lactobacillus rhamnosus cells from whey permeate with nutrient supplements. Food and Bioprocess Technology. doi:10.1007/s11947-010-0426-1.

  • Davison, B. E., Llanos, R. L., Cancilla, M. R., Redman, N. C., & Hillier, A. J. (1995). Current research on the genetics of lactic acid production in lactic acid bacteria. International Dairy Journal, 5, 763–784.

    Article  Google Scholar 

  • Fonteles, T. V., Costa, M. G. M., Jesus, A. L. T., & Rodrigues, S. (2011). Optimization of the fermentation of cantaloupe juice by Lactobacillus casei NRRL B-442. Food and Bioprocess Technology. doi:10.1007/s11947-011-0600-0.

  • Guilherme, A. A., Dornelles, A. S., Pinto, G. A. S., Brito, E. S., & Rodrigues, S. (2009). Quality evaluation of mesquite pods and cashew apple syrups. Journal of Food Process Engineering, 32, 606–627.

    Article  Google Scholar 

  • Laopaiboon, P., Thani, A., Leelavatcharamas, V., & Laopaiboon, L. (2010). Acid hydrolysis of sugarcane bagasse for lactic acid production. Bioresource Technology, 101, 1036–1043.

    Article  CAS  Google Scholar 

  • Litchfield, J. H. (1996). Microbiological production of lactic acid. Advances in Applied Microbiology, 42, 45–95.

    Article  CAS  Google Scholar 

  • Himmelblau, D. M. (1970). Process analysis by statistical methods. New York, USA: Wiley.

    Google Scholar 

  • Hofvendahl, K., & Hahn-Hägerdal, B. (2000). Factors affecting the fermentative lactic acid production from renewable resources. Enzyme and Microbiological Technology, 26, 87–107.

    Article  CAS  Google Scholar 

  • Hong, A. A., Cheng, K. K., Peng, F., Zhou, S., Sun, Y., Liu, C. M., et al. (2009). Strain isolation and optimization of process parameters for bioconversion of glycerol to lactic acid. Journal of Chemical Technology and Biotechnology, 84, 1576–1581.

    Article  CAS  Google Scholar 

  • Honorato, T. L., Rabelo, M. C., Gonçalves, L. R. B., Pinto, G. A. S., & Rodrigues, S. (2007). Fermentation of cashew apple juice to produce high added value products. World Journal of Microbiology and Biotechnology, 23, 1409–1415.

    Article  CAS  Google Scholar 

  • Luedeking, P., & Piret, E. L. (1959). A kinetic study of the lactic acid fermentation. Batch process at controlled pH. Journal of Biochemical and Microbiological Technology and Engineering, 1, 393–412.

    Article  CAS  Google Scholar 

  • Lunelli, B. H., Andrade, R. R., Atala, D. I. P., Maciel, M. R. W., Maugeri Filho, F., & Maciel, F. R. (2010). Production of lactic acid from sucrose: strain selection, fermentation, and kinetic modeling. Applied Biochemistry and Biotechnology, 161, 227–237.

    Article  CAS  Google Scholar 

  • Nancib, N., Nancib, A., Boudjelal, A., Benslimane, C., Blanchard, F., & Boudrant, J. (2001). The effect of supplementation by different nitrogen sources on the production of lactic acid from date juice by Lactobacillus casei subsp. rhamnosus. Bioresource Technology, 78, 149–153.

    Article  CAS  Google Scholar 

  • Nancib, A., Nancib, N., & Boudrant, J. (2009). Production of lactic acid from date juice extract with free cells of single and mixed cultures of Lactobacillus casei and Lactococcus lactis. World Journal of Microbiology and Biotechnology, 25, 1423–1429.

    Article  CAS  Google Scholar 

  • Nandasana, A. D., & Kumar, S. (2008). Kinetic modeling of lactic acid production from molasses using Enterococcus faecalis RKY1. Biochemical Engineering Journal, 38, 277–284.

    Article  CAS  Google Scholar 

  • Pereira, A. L. F., Maciel, T. C., & Rodrigues, S. (2011). Probiotic cashew apple juice. Probiotic beverage from cashew apple juice fermented with Lactobacillus casei. Food Research International, 44, 1276–1283.

    Article  CAS  Google Scholar 

  • Rabelo, M. C., Honorato, T. L., Gonçalves, L. R. B., Pinto, G. A., & Rodrigues, S. (2009). Optimization of enzymatic synthesis of isomalto-oligosaccharides. Journal of Food Biochemistry, 33, 342–354.

    Article  CAS  Google Scholar 

  • Rojan, P. J., Nampoothiri, K. M., & Pandey, A. (2007). Fermentative production of lactic acid from biomass: an overview on process development and future perspectives. Applied Microbiology and Biotechnology, 74, 524–534.

    Article  Google Scholar 

  • Ryu, H. W., Yun, J. S., & Wee, Y. J. (2003). Lactic acid. In Pandey (Ed.), Concise encyclopedia of bioresource technology (pp. 635–644). New York, USA: Haworth.

    Google Scholar 

  • Silveira, M. S., Fontes, C. P. M. L., Guilherme, A. A., Fernandes, F. A. N., & Rodrigues, S. (2010). Cashew apple juice as substrate for lactic acid production. Food and Bioprocess Technology. doi:10.1007/s11947-010-0382-9.

  • Tejayadi, S., & Cheryan, M. (1995). Lactic acid from cheese whey permeate. Productivity and economics of a continuous membrane bioreactor. Applied Microbiology and Biotechnology, 43, 242–248.

    Article  CAS  Google Scholar 

  • VickRoy, T. B. (1985). Lactic acid. In Moo-Young (Ed.), Comprehensive biotechnology (pp. 761–776). New York, USA: Pergamon.

    Google Scholar 

  • Vinderola, C. G., & Reinheimer, J. A. (2000). Enumeration of Lactobacillus casei in the presence of L. acidophilus. Bifidobacteria and lactic starter bacteria in fermented dairy products. International Dairy Journal, 10, 271–275.

    Article  Google Scholar 

  • Wang, Q., Narita, J. Y., Xie, W., Ohsumi, Y., Kusano, K., Shirai, Y., et al. (2002). Effects of anaerobic/aerobic incubation and storage temperature on preservation and deodorization of kitchen garbage. Bioresource Technology, 84, 213–220.

    Article  CAS  Google Scholar 

  • Wee, Y. J., Kim, J. N., & Ryu, H. W. (2006). Biotechnological production of lactic acid and its recent applications. Food Technology and Biotechnology, 44, 163–172.

    CAS  Google Scholar 

  • Yen, H. W., & Lee, Y. C. (2009). Production of lactic acid from raw sweet potato powders by Rhizopus oryzae immobilized in sodium alginate capsules. Applied Biochemistry and Biotechnology. doi:10.1007/s12010-009-8884-5.

  • Youssef, C. B., Goma, G., & Olmos-Dichara, A. (2005). Kinetic modeling of Lactobacillus casei ssp. rhamnosus growth and lactic acid production in batch cultures under various medium conditions. Biotechnology Letters, 27, 1785–1789.

    Article  Google Scholar 

  • Zhang, Z. Y., Jin, B., & Kelly, J. M. (2007). Production of lactic acid from renewable materials by Rizopus fungi. Biochemical Engineering Journal, 35, 251–263.

    Article  CAS  Google Scholar 

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Acknowledgments

The authors thank the financial support and scholarship from the Brazilian funding institute CNPq and CAPES.

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Authors and Affiliations

  1. Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Bloco 709, 60455-760, Fortaleza, CE, Brazil

    Alexandre A. Guilherme & Fabiano A. N. Fernandes

  2. Departamento de Tecnologia de Alimentos, Universidade Federal do Ceará, Campus do Pici, Bloco 858, 60455-760, Fortaleza, CE, Brazil

    Mariana S. Silveira, Claudia P. M. L. Fontes & Sueli Rodrigues

Authors
  1. Alexandre A. Guilherme
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  2. Mariana S. Silveira
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  3. Claudia P. M. L. Fontes
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  4. Sueli Rodrigues
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  5. Fabiano A. N. Fernandes
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Correspondence to Fabiano A. N. Fernandes.

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Guilherme, A.A., Silveira, M.S., Fontes, C.P.M.L. et al. Modeling and Optimization of Lactic Acid Production using Cashew Apple Juice as Substrate. Food Bioprocess Technol 5, 3151–3158 (2012). https://doi.org/10.1007/s11947-011-0670-z

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  • DOI: https://doi.org/10.1007/s11947-011-0670-z

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