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Production of D-lactic acid by L. delbrueckii growing on orange peel waste hydrolysates and model monosaccharide solutions: effects of pH and temperature on process kinetics

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Abstract

D-lactic acid is a key monomer of polylactic acid (PLA) and other biopolymers. In this work, the effects of temperature and pH on the D-lactate production with Lactobacillus delbrueckii in model sugar solutions and real orange peel waste hydrolysates (OPWH) have been studied and modeled with a non-structured non-segregated kinetic model. Lactobacillus delbrueckii ssp. delbrueckki CECT 286 yields 0.88 g/g of D-lactate (purity 97%) with a productivity of 2.56 g/L·h in the best tested conditions (40 °C and pH 5.8). The proposed kinetic model is able to describe the performed runs, and its kinetic parameters reflect the influence of temperature and pH on the uptake of monosaccharides, D-lactic acid production, and bacterium growth. Moreover, it can be applied to simulate runs with real OPWH supplemented with MRS broth or corn steep liquor (CSL) as a nitrogen source.

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Abbreviations

Cj :

Referred to concentrations of compound j (gj/L).

D-LA:

D-lactic acid.

F:

Fischer-F, statistical parameter.

Ks :

Saturation constant for the glucose (gG/L).

kP :

Kinetic constant for D-lactic acid production from fructose and galactose (gP.L/gF + Gl.gX.h).

M:

Number of parameters of a proposed model.

N:

Total number of experimental data used for fitting.

P:

Productivity of D-lactic acid (g/L·h).

ri :

Reaction rate of the reaction i (g/L·h).

RMSE:

Residual mean squared error.

SSQl :

Sum of squared residues respect to the calculated value of “l” variable.

SSQlmean :

Squared sum of deviations between the experimental and the mean score respect to The calculated values of the variable.

SSR:

Sum of squared residuals.

t:

Time (h).

VE:

Variation explained of a nonlinear regression fitting (%).

YP,S :

Yield of D-lactic acid referred to the total consumption of carbon sources (g/g).

yi :

Experimental or calculated values of “i” variables.

μm :

Maximum biomass growth rate (h−1).

νij :

Coefficients in the reaction scheme (eqs. (3) and (4)) (gi·gj−1).

A:

Referred to arabinose.

calc:

Referred to the calculated value of a variable from the model.

F:

Referred to fructose.

G:

Referred to glucose.

Gl:

Referred to galactose.

P:

Referred to product (D-lactic acid).

S:

Referred to the sum of all the substrates.

tab:

Referred to a F-value given by F-test tables for a confidence interval.

X:

Referred to biomass.

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Funding

This work has been supported by the Spanish MICINN under contract PCIN-2013-021-C02-01.

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Authors and Affiliations

  1. Chemical and Materials Engineering Department, Chemical Sciences Faculty, Complutense University of Madrid, 28040, Madrid, Spain

    Isabel de la Torre, Miguel Ladero & Victoria E. Santos

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  1. Isabel de la Torre
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  2. Miguel Ladero
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Correspondence to Miguel Ladero.

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de la Torre, I., Ladero, M. & Santos, V.E. Production of D-lactic acid by L. delbrueckii growing on orange peel waste hydrolysates and model monosaccharide solutions: effects of pH and temperature on process kinetics. Biomass Conv. Bioref. 9, 565–575 (2019). https://doi.org/10.1007/s13399-019-00396-3

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