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Isolation and Screening of Lipase-Producing Fungi with Hydrolytic Activity

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Abstract

Lipases are enzymes that can be secreted by several microorganisms, making interesting the biodiversity exploration for searching new microorganisms able to produce these enzymes. Many agro-industrial residues can be used as potential substrates for production of enzymes. The main objective of this work was the isolation and screening of microorganisms with potential to produce lipases. Among 24 fungi, five were selected as good lipase producers using tributyrin on agar plates and solid state fermentation of soybean bran. Two of them were isolated from soil samples, another two from soybean bran, and one from dairy products. These fungi were identified by microcultivation technique as from Penicillium and Aspergillus genera. Through random amplified polymorphic DNA technique, the most promising strains could be genetically discriminated, selecting two fungi as good lipase producers but genetically different. One isolated from soybean bran could hydrolyze efficiently triglycerides with fatty acids with different chain length. Another isolated from dairy products was only effective to hydrolyze triglycerides with long-chain fatty acids. Two distinct groups could be verified by means of this technique, comprising the most productive strains and the lowest or nonproductive ones in terms of hydrolytic activity.

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Acknowledgments

The authors thank CAPES/PROCAD, CNPq, and Intecnial for the financial support of this work and scholarships.

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Authors and Affiliations

  1. Programa de Pós-graduação em Engenharia de Alimentos, Universidade Regional Integrada do Alto Uruguai e das Missões-Campus de Erechim, Av. Sete de Setembro, 1621, 99700-000, Erechim, RS, Brazil

    Nara Griebeler, André E. Polloni, Daniela Remonatto, Francieli Arbter, Renata Vardanega, Jeferson L. Cechet, Marco Di Luccio, Débora de Oliveira, Helen Treichel & Rogério L. Cansian

  2. Departamento de Engenharia Química e de Alimentos, Universidade Federal de Santa Catarina, UFSC, Florianópolis, SC, Brazil

    Elisandra Rigo & Jorge L. Ninow

Authors
  1. Nara Griebeler
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  2. André E. Polloni
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  3. Daniela Remonatto
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  4. Francieli Arbter
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  5. Renata Vardanega
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  7. Marco Di Luccio
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  8. Débora de Oliveira
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  9. Helen Treichel
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  10. Rogério L. Cansian
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  11. Elisandra Rigo
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Correspondence to Marco Di Luccio.

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Griebeler, N., Polloni, A.E., Remonatto, D. et al. Isolation and Screening of Lipase-Producing Fungi with Hydrolytic Activity. Food Bioprocess Technol 4, 578–586 (2011). https://doi.org/10.1007/s11947-008-0176-5

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  • DOI: https://doi.org/10.1007/s11947-008-0176-5

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