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Critical Assessment of In Vitro and In Vivo Models to Study Marrow Adipose Tissue

  • Bone Marrow and Adipose Tissue (G Duque and B Lecka-Czernik, Section Editors)
  • Published:
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Abstract

Purpose of the Review

The purpose of this review is to describe the in vitro and in vivo methods that researchers use to model and investigate bone marrow adipocytes (BMAds).

Recent Findings

The bone marrow (BM) niche is one of the most interesting and dynamic tissues of the human body. Relatively little is understood about BMAds, perhaps in part because these cells do not easily survive flow cytometry and histology processing and hence have been overlooked. Recently, researchers have developed in vitro and in vivo models to study normal function and dysfunction in the BM niche. Using these models, scientists and clinicians have noticed that BMAds, which form bone marrow adipose tissue (BMAT), are able to respond to numerous signals and stimuli, and communicate with local cells and distant tissues in the body.

Summary

This review provides an overview of how BMAds are modeled and studied in vitro and in vivo.

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References

Papers of particular interest, published recently, have been highlighted as: • Of importance •• Of major importance

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Acknowledgments

We acknowledge Servier Medical Art (https://smart.servier.com) for providing images of mechanical testing and bone components.

Funding

Support was from the NIH’s National Institute of General Medical Science from the Phase I COBRE in Metabolic Networks (P20GM121301) and the U54GM115516 administrative core. The author's work is also supported by an American Cancer Society Research Scholar Grant (RSG-19-037-01-LIB), a pilot project from the American Cancer Society (Research Grant #IRG-16-191-33; Reagan PI), a pilot from Dana-Farber Cancer Institute, a grant from the Kane Foundation, an R24 grant DK092759–01, and start-up funds from the Maine Medical Center Research Institute.

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  1. Center for Molecular Medicine and Center for Translational Research, Maine Medical Center Research Institute, 81 Research Drive, Scarborough, ME, 04074, USA

    Michaela R. Reagan

  2. University of Maine Graduate School of Biomedical Science and Engineering, Orono, ME, USA

    Michaela R. Reagan

  3. School of Medicine and Graduate School of Biomedical Sciences, Tufts University, Boston, MA, USA

    Michaela R. Reagan

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Correspondence to Michaela R. Reagan.

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Michaela Reagan reports grants from NIH and the Kane Foundation, and some funding from UCB Biopharma (Brussells, Belgium) during the conduct of the study.

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Reagan, M.R. Critical Assessment of In Vitro and In Vivo Models to Study Marrow Adipose Tissue. Curr Osteoporos Rep 18, 85–94 (2020). https://doi.org/10.1007/s11914-020-00569-4

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