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Gynogenesis Assessment Using Microsatellite Genetic Markers in Turbot (Scophthalmus maximus)

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Abstract

Gynogenesis was assessed by different methods in 2 families of gynogenetic offspring in turbot (Scophthalmus maximus). Karyotype analysis in embryos and larvae demonstrated high accuracy in estimation of ploidy level, but performance was uneven given the low quality and number of plates obtained. The use of silver staining to estimate the number of nucleoli per nucleus resulted in a straightforward and easy method to evaluate the ploidy of the samples studied. However, the existence of a nucleolus organizer region polymorphism in turbot determined a small error in ploidy estimation, important when checking ploidy in specific individuals. The use of a set of 11 highly variable microsatellite loci proved to be a powerful method to confirm the exclusive maternal inheritance to gynogenetic offspring in turbot, with probabilities of detection of putative paternal genetic contribution above 99.99%.

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Acknowledgements

This work has been supported by FEDER grant 1FD1997-2404.

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Authors and Affiliations

  1. Departamento de Genética, Universidad de Santiago de Compostela, 27002 Lugo, Spain

    J. Castro, C. Bouza, L. Sánchez & P. Martínez

  2. Instituto Español de Oceanografia (IEO), Centro Oceanográfico de Vigo, 36280 Vigo, Spain

    R. M. Cal

  3. Consejo Superior de Investigaciones Cientificas (CSIC), Institut de Ciències del Mar, 08003 Barcelona,, Spain

    F. Piferrer

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  1. J. Castro
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Correspondence to P. Martínez.

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Castro, J., Bouza, C., Sánchez, L. et al. Gynogenesis Assessment Using Microsatellite Genetic Markers in Turbot (Scophthalmus maximus) . Mar. Biotechnol. 5, 584–592 (2003). https://doi.org/10.1007/s10126-003-0004-x

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