Abstract
The activities of antioxidative enzymes and the concentration of malondialdehyde were assayed in cucumber leaves (Cucumis sativus L.) 0, 6, 12, 24, 48, 72, and 96 h after the cucumber seedlings were infested by Bemisia tabaci (Gennadius). The results indicated that the activities of antioxidative enzymes were increased after herbivore infestation, including superoxide dismutase (SOD), and peroxidase (POD), and the levels of malondialdehyde (MDA), which is a product of membrane lipid peroxidation in the leaves. The enzymes and MDA showed peaks of different activity levels at 24 and 48 h after the infestation. SOD activity reached the highest peak, 10.4% higher than control, at 24 h, POD activity reached the highest peak, 213.2% higher than control, at 6 h, catalase (CAT) activity was not statistically significant compared with the control, and MDA content reached the highest peak, 59.9% higher than control, at 48 h. The results suggested that the enhanced activities of antioxidative enzymes and MDA content may contribute to bioprotection of cucumber plants against B. tabaci infestation.
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Agrawal AA, Strauss SY, Stout MJ (1999) Costs of induced responses and tolerance to herbivory in male and female fitness components of wild radish. Evol Int J Org Evol 53:1093–1104. doi:10.2307/2640814
Allison SD, Schultz JC (2004) Differential activity of peroxidase isozymes in response to wounding, gypsy moth, and plant hormones in northern red oak (Quercus rubra L.). J Chem Ecol 30(7):1363–1379. doi:10.1023/B:JOEC.0000037745.66972.3e
Asada K (2006) Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiol 141(2):391–396. doi:10.1104/pp.106.082040
Bailly C, Benamar A, Corbineau F et al (1996) Changes in malondialdehyde content and in superoxide dismutase, catalase and glutathione reductase activities in sunflower seed as related to deterioration during accelerated aging. Physiol Plant 97:104–110. doi:10.1111/j.1399-3054.1996.tb00485.x
Baldwin IT, Ohnmeiss TE (1994) Coordination of photosynthetic and alkaloidal responses to damage in uninducible and inducible Nicotiana sylvestris. Ecology 75:1003–1014. doi:10.2307/1939424
Bellotti AC, Arias B (2001) Host plant resistance to whiteflies with emphasis on cassava as a case study. Crop Prot 20:813–823. doi:10.1016/S0261-2194(01)00113-2
Berglund T, Ohlsson AB (1995) Defensive and secondary metabolism in plant tissue cultures, with special reference to nicotinamide, glutathione and oxidative stress. Plant Cell Tiss Org Cult 43:137–145. doi:10.1007/BF00052169
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254. doi:10.1016/0003-2697(76)90527-3
Byrne DN, Bellows TS Jr (1991) Whitefly biology. Annu Rev Entomol 36:431–457. doi:10.1146/annurev.en.36.010191.002243
Chen YW, Dou CH, Zhang XH, Li XR (2004) Phsyological variation of damaged leaves of pears by Eriophyes pyri Pagenstecher. J Lanzhou Univ 40(1):68–71
Cipollini DF (1997) Wind-induced mechanical stimulation increases pest resistance in common bean. Oecologia 111:84–90. doi:10.1007/s004420050211
Felton GW, Donato K, Del Vecchio RJ, Duffey SS (1989) Activation of plant foliar oxidases by insect feeding reduces nutritive quality of foliage for noctuid herbivores. J Chem Ecol 15:2667–2694. doi:10.1007/BF01014725
Halliwell B, Gutteridge JMC (1999) Free radicals in biology and medicine, 3rd edn. Oxford University Press
Howe GA, Schilmiller AL (2002) Oxylipin metabolism in response to stress. Curr Opin Plant Biol 5:230–236. doi:10.1016/S1369-5266(02)00250-9
Kessler A, Baldwin IT (2001) Defensive function of herbivore-induced plant volatile emissions in nature. Science 291:2141–2144. doi:10.1126/science.291.5511.2141
Liu YQ, Jiang L, Sun LH, Wang CM, Zhai HQ, Wan JM (2005) Changes in some defensive enzyme activity induced by the piercing-sucking of brown planthopper in rice. J Plant Physiol Mol Biol 31(6):643–650
Low PS, Merida JR (1996) The oxidative burst in plant defense: function and signal transduction. Physiol Plant 96:533–542. doi:10.1111/j.1399-3054.1996.tb00469.x
Maffei ME, Mithofer A, Arimura GI, Uchtenhagen H, Bossi S, Bertea CM et al (2006) Effects of feeding Spodoptera littoralis on lima bean leaves. III. Membrane depolarization and involvement of hydrogen peroxide. Plant Physiol 140(3):1022–1035. doi:10.1104/pp.105.071993
Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7(9):405–410. doi:10.1016/S1360-1385(02)02312-9
Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxidation in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351. doi:10.1016/0003-2697(79)90738-3
Palumbo JC, Horowitz AR, Prabhaker N (2001) Insecticidal control and resistance management for Bemisia tabaci. Crop Prot 20:739–765. doi:10.1016/S0261-2194(01)00117-X
Scandalios JG (1993) Oxygen stress and superoxide dismutases. Plant Physiol 101:7–12
Tscharntke T, Thiessen S, Dolch R, Boland W (2001) Herbivory, induced resistance, and interplant signal transfer in Alnus glutinosa. Biochem Syst Ecol 29:1025–1047. doi:10.1016/S0305-1978(01)00048-5
Zimmerlin A, Wojtaszek P, Bolwell GP (1994) Synthesis of dehydrogenation polymers of ferulic acid with high specificity by a purified cell-wall peroxidase from French bean (Phaseolus vulgaris L.). Biochem J 299:747–753
Acknowledgements
We thank J. C. Reese (Department of Entomology, Kansas State University) for valuable suggestions that greatly improved the manuscript. We also acknowledge the helpful comments of two anonymous reviewers and the editor. This research was supported by the National Basic Research Program of Ministry of Science and Technology, China (973 Program, 2006CB102005-3).
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Zhang, SZ., Hua, BZ. & Zhang, F. Induction of the activities of antioxidative enzymes and the levels of malondialdehyde in cucumber seedlings as a consequence of Bemisia tabaci (Hemiptera: Aleyrodidae) infestation. Arthropod-Plant Interactions 2, 209–213 (2008). https://doi.org/10.1007/s11829-008-9044-5
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DOI: https://doi.org/10.1007/s11829-008-9044-5