Abstract
Cell suspension cultures of Linum album were developed from internode portions of in vitro germinated plant in Gamborg’s B5 medium supplemented with 0.4 mg naphthalene acetic acid/l. The highest biomass was 8.5 g/l with podophyllotoxin and 6-methoxypodophyllotoxin at 29 and 1.9 mg/l, respectively after 12 d cultivation. Co-cultures of L. album cells with axenically cultivable arbuscular mycorrhiza-like fungi, Piriformospora indica and Sebacina vermifera, were established for the first time. These enhanced podophyllotoxin and 6-methoxypodophyllotoxin production by about four- and eight-fold, respectively, along with a 20% increase in biomass compared to the control cultures.
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The authors are thankful to Prof. Ajit Varma, Amity Institute of Herbal and Microbial Studies, Noida, India for providing fungal cultures.
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Intellectual property rights of the work is covered under Patent application no. 1266/DEL/2007.
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Baldi, A., Jain, A., Gupta, N. et al. Co-culture of arbuscular mycorrhiza-like fungi (Piriformospora indica and Sebacina vermifera) with plant cells of Linum album for enhanced production of podophyllotoxins: a first report. Biotechnol Lett 30, 1671–1677 (2008). https://doi.org/10.1007/s10529-008-9736-z
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DOI: https://doi.org/10.1007/s10529-008-9736-z