Abstract
This communication describes for the first time an efficient and reproducible protocol for large-scale multiplication of Bambusa nutans. Nodal segments collected from field-grown clumps and cultured on Murashige and Skoog (MS) medium supplemented with 4.4 μM benzylaminopurine (BA) and 2.32 μM kinetin (Kin) gelled with 0.2% gelrite yielded 80% aseptic cultures with 100% bud-break. The in vitro-formed shoots obtained after bud-break were successfully multiplied in MS liquid medium supplemented with 13.2 μM BA, 2.32 μM Kin, and 0.98 μM indole-3-butyric acid (IBA). Sub-culturing of shoots every 3 weeks on fresh multiplication medium yielded a consistent proliferation rate of 3.5-fold. Shoot clusters containing three to five shoots were successfully rooted with 100% success on half-strength MS liquid medium supplemented with 9.8 μM IBA, 2.85 μM indole-3-acetic acid (IAA), 2.68 μM naphthaleneacetic acid (NAA), and 3% sucrose. Plantlets grown in vitro were acclimatized and subsequently transferred to the field. Inter-simple sequence repeat analysis has confirmed the genetic uniformity of the tissue-cultured plants up to 27 passages.
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References
Arya S, Rana PK, Sharma R, Arya ID (2006) Tissue culture technology for rapid multiplication of Dendrocalamus giganteus Munro. Indian For 132:345–357
Bornet B, Branchard M (2001) Nonanchored Inter Simple Sequence Repeat (ISSR) markers: reproducible and specific tools for genome fingerprinting. Plant Mol Biol Rep 19:209–215
Chowdhury P, Das M, Sikdar SR, Pal A (2004) Influence of the physiological age and position of the nodal explants on micropropagation of field-grown Dendrocalamus strictus Nees. Plant Cell Biotech Mol Biol 5:45–50
Das M, Pal A (2005) In vitro regeneration of Bambusa balcooa Roxb.: factors affecting changes of morphogenetic competence in the axillary buds. Plant Cell Tissue Organ Cult 81:109–112
Debergh P (1983) Effects of agar brand and concentration on the tissue culture medium. Physiol Plant 59:270–276
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Driver JA, Suttle GR (1987) Nursery handling of propagules. In: Bonga JM, Durzan DJ (eds) Cell and tissue culture in forestry. Kluwer, Dordrecht, pp 320–335
Jimenez VM, Castillo J, Tavares E, Guevara E, Montiel M (2006) In vitro propagation of the neotropical giant bamboo, Guadua angustifolia Kunth, through axillary shoot proliferation. Plant Cell Tissue Organ Cult 86:389–395
Kalia S, Kalia RK, Sharma SK (2004) In vitro regeneration of an indigenous bamboo (Bambusa nutans) from internode and leaf explant. J Bamboo Rattan 3:217–228
Kapoor P, Rao IU (2006) In vitro rhizome induction and plantlet formation from multiple shoots in Bambusa bambos var gigantea Bennet and Gaur by using growth regulators and sucrose. Plant Cell Tissue Organ Cult 85:211–217
Mishra Y, Patel PK, Yadav S, Shirin F, Ansari SA (2008) A micropropagation system for cloning of Bambusa tulda Roxb. Sci Hortic 115(3):315–318
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–479
Nagaoke T, Ogihara Y (1997) Applicability of Inter-Simple Sequence Repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers. Theor Appl Genet 94:597–602
Negi D, Saxena S (2009) Ascertaining clonal fidelity of tissue culture raised plants of Bambusa balcooa Roxb. using inter simple sequence repeat markers. New For 40(1):1–8. doi:10.1007/s11056-009-9182-3
Ramanayake SMSD, Wanniarachchi WAVR, Tennakoon TMA (2001) Axillary shoot proliferation and in vitro flowering in an adult giant bamboo, Dendrocalamus giganteus Wall ex. Munro. In Vitro Cell Dev Biol Plant 37:667–671
Sanjaya, Rathore TS, Ravishankar Rai V (2005) Micropropagation of Pseudoxytenanthera stocksii Munro. In Vitro Cell Dev Biol Plant 41:333–337
Saxena S (1990) In vitro propagation of the bamboo (Bambusa tulda Roxb.) through shoot proliferation. Plant Cell Rep 9:431–434
Saxena S, Bhojwani SS (1993) In vitro clonal multiplication of 4-year old plants of the bamboo, Dendrocalamus longispathus Kurz. In Vitro Cell Dev Biol Plant 29:135–142
Shenoy VB, Vasil IK (1992) Biochemical and molecular analysis of plants derived from embryogenic tissue cultures of napier grass (Pannisetum purpureum K Schum). Theor Appl Genet 83:947–955
Singh S, Ansari SA, Kumar P (2002) Clonal propagation of Bambusa nutans through culm and culm-branch cuttings. Indian For 128:35–40
Stapleton C (1998) Form and function in the bamboo rhizome. J Am Bamboo Soc 12:21–29
Tewari DN (1992) A monograph on bamboo. International Book Distributors, Dehra Dun
Tornero OP, Egea J, Olmos E, Burgos L (2001) Control of hyperhydricity in micropropagated apricot cultivars. In Vitro Cell Dev Biol Plant 37:250–254
Tsumara Y, Ohba K, Strauss SH (1996) Diversity and inheritance of inter-simple sequence repeat polymorphisms in Douglas-fir (Pseudotsuga menziesii) and sugi (Cryptomeria japonica). Theor Appl Genet 92:40–45
Yasodha R, Sumathi R, Malliga P, Gurumurthi K (1997) Genetic enhancement and mass production of quality propagules of Bambusa nutans and Dendrocalamus membranaceous. Indian For 123:303–306
Yasodha R, Kamala S, Anand Kumar SP, Durai Kumar P, Kalaiarasi K (2008) Effect of glucose on in vitro rooting of mature plants of Bambusa nutans. Sci Hortic 116(1):113–116
Zimmerman TW, Rogers SMD, Cobb BG (1991) Controlling vitrification of Petunia in vitro. In Vitro Cell Dev Biol Plant 27:165–167
Ziv M (1991) Quality of micropropagated plants—vitrification. In Vitro Cell Dev Biol Plant 27:64–69
Acknowledgments
Our profound thanks to Dr. S. A. Ansari, Tropical Forest Research Institute (TFRI), Jabalpur, for providing us with research material of B. nutans and to Dr. R. K. Pachauri, Director General, The Energy and Resources Institute (TERI), for infrastructure support. Thanks are due to Dr. Shashi Bhushan Tripathi and Mr. Madan Singh Negi for their help in planning the clonal fidelity experiments. The financial support of Council of Scientific and Industrial Research, Government of India, extended to Ms. Divya Negi is also gratefully acknowledged.
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Negi, D., Saxena, S. In vitro propagation of Bambusa nutans Wall. ex Munro through axillary shoot proliferation. Plant Biotechnol Rep 5, 35–43 (2011). https://doi.org/10.1007/s11816-010-0154-z
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DOI: https://doi.org/10.1007/s11816-010-0154-z