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Controlling vitrification of petunia in vitro

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Summary

Nodal segments from in vitro culturedPetunia hybrida were grown under varying cultural conditions. The origin of nodal explants influenced vitrification. Basal segments formed a higher percentage of vitreous shoots than did the upper nodes. A method was developed for including polyethylene glycol with Gelrite to obtain gelled media of varying water potentials. Media water potential from −0.31 to −1.2 MPa had no effect on controlling the level of vitrification. Gelrite promoted vitrification but GIBCO agar, alone or in combination with Gelrite, reduced its occurrence. Lowering media NH4 content reduced vitrification, whereas sealing culture vessels with parafilm increased it. As it is now possible to control normal and vitreous plant development inPetunia, this can be used as a model system for studying the physiology and biochemistry of this developmental abnormality.

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Authors and Affiliations

  1. Department of Horticultural Sciences, Texas A&M University, 77843, College Station, Texas

    Thomas W. Zimmerman, Suzanne M. D. Rogers & B. Greg Cobb

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  1. Thomas W. Zimmerman
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  2. Suzanne M. D. Rogers
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  3. B. Greg Cobb
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Zimmerman, T.W., Rogers, S.M.D. & Cobb, B.G. Controlling vitrification of petunia in vitro. In Vitro Cell Dev Biol - Plant 27, 165–167 (1991). https://doi.org/10.1007/BF02632211

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  • DOI: https://doi.org/10.1007/BF02632211

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