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In vitro regeneration from cotyledon explants in figleaf gourd (Cucurbita ficifolia Bouché), a rootstock for Cucurbitaceae

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Abstract

An efficient plant regeneration system has been developed for figleaf gourd (Cucurbita ficifolia Bouché), which is exclusively used as a rootstock for cucumber. The protocol is based on results obtained from a series of culture experiments involving different parts of the cotyledons and various media. The culture of cotyledon explants was critical for the enhancement of shoot regeneration frequency. The lower parts of the cotyledon excised at the plumule base were found to display a markedly enhanced production of adventitious shoots compared to other cotyledon regions. Culture in silver nitrate-supplemented Murashige and Skoog (MS) medium was not beneficial for shoot regeneration and suppressed root regeneration. Efficient shoot regeneration was obtained on MS medium containing 1.0 mg l−1 zeatin and 0.1 mg l−1 indole-3-acetic acid. Regenerated shoots successfully elongated and rooted in medium containing 0.1 mg l−1 1-naphthaleneacetic acid after 10–15 days of subculturing. The plantlets were satisfactorily acclimatized in a greenhouse and grew into normal plants without any morphological alterations.

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Acknowledgments

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2009-0064836).

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Authors and Affiliations

  1. College of Ecology and Environmental Science, Kyungpook National University, Sangju, 742-711, Republic of Korea

    Kyung-Min Kim, Chang Kil Kim & Jeung-Sul Han

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  1. Kyung-Min Kim
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  2. Chang Kil Kim
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  3. Jeung-Sul Han
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Correspondence to Jeung-Sul Han.

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Kim, KM., Kim, C.K. & Han, JS. In vitro regeneration from cotyledon explants in figleaf gourd (Cucurbita ficifolia Bouché), a rootstock for Cucurbitaceae. Plant Biotechnol Rep 4, 101–107 (2010). https://doi.org/10.1007/s11816-009-0122-7

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  • DOI: https://doi.org/10.1007/s11816-009-0122-7

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