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Antifungal mechanisms of ε-poly-L-Lysine with different molecular weights on Saccharomyces cerevisiae

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Abstract

ε-Poly-L-lysine (ε-PL) is a natural antimicrobial cationic peptide. Antimicrobial activity of ε-PL is closely related to its molecular weight (Mw). However, the antimicrobial mechanisms of ε-PL with different Mws are still vague. In this study, Saccharomyces cerevisiae was used as the model system to analyze the mechanism from these three aspects: cell wall, cell membrane, and metabolism. The results showed that high-Mw ε-PL (1-3 kDa and >3 kDa) and commercial ε-PL product caused cell wall lesions, and significantly improved cell membrane permeability compared to low-Mw ε-PL (<1 kDa), resulting in leaking of the protoplasm through the pores and cell death. Furthermore, metabolomics analysis showed that high-Mw ε-PL (1-3 kDa and >3 kDa) and product displayed higher inhibition effect on the glycolysis pathway and tricarboxylic cycle than that of low-Mw ε-PL (<1 kDa).

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Acknowledgements

This research was funded by the National Key R&D Program of China (Project 2018YFD0400205), the Tianjin Municipal Commission of Education Research Plan (2017KJ011), Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control (ZXKF20180101), and Tianjin Natural Science Foundation (18JCQNJC78600).

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Authors and Affiliations

  1. State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, P. R. China

    Ying Hou, Fengping Wang, Zhilei Tan, Jiandong Cui & Shiru Jia

  2. College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, P. R. China

    Ying Hou, Fengping Wang, Zhilei Tan, Jiandong Cui & Shiru Jia

Authors
  1. Ying Hou
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  2. Fengping Wang
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  3. Zhilei Tan
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  4. Jiandong Cui
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  5. Shiru Jia
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Correspondence to Jiandong Cui or Shiru Jia.

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Hou, Y., Wang, F., Tan, Z. et al. Antifungal mechanisms of ε-poly-L-Lysine with different molecular weights on Saccharomyces cerevisiae. Korean J. Chem. Eng. 37, 482–492 (2020). https://doi.org/10.1007/s11814-019-0466-9

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  • DOI: https://doi.org/10.1007/s11814-019-0466-9

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