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Immobilization of Candida rugosa lipase onto an eco-friendly support in the presence of ionic liquid

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Abstract

Candida rugosa lipase (CRL) was immobilized on an eco-friendly support poly(3-hydroxybutyrate-co-hydroxyvalerate) (PHBV), by physical adsorption, using different ionic liquids (ILs) as immobilization additives. This was to investigate the influence of cationic core ([C4mpy]Cl, [C4min]Cl), of anions ([C4min]Cl, [C4min]N(CN)2, [C4min]Tf2N), and of cation chain length ([C2min]Tf2N, [C4min]Tf2N) in the immobilization process. The immobilized biocatalysts (IB) were characterized with respect to the morphological, physico-chemical properties, total activity recovery yield (Ya), and biochemical properties of more efficient IB were evaluated. Initially, it was found that the change of cationic core did not influence in Ya compared to the control. With change of anions, it was seen that the best result was obtained for the more hydrophobic anion (Tf2N), and finally increasing the cation chain length increased Ya. IB most efficient with [C4min]Tf2N obtained 78 % of Ya, more than twice the control value (30 %) and a considerable enhancement of operational stability compared with the control.

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Acknowledgments

The authors are grateful for the financial support from the Brazilian research funding agencies CAPES, CNPq, and FAPITEC/SE.

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Authors and Affiliations

  1. Universidade Tiradentes, Av. Murilo Dantas, 300, Farolândia, Aracaju, SE, 49032-490, Brazil

    Rebeca Y. Cabrera-Padilla, Milena C. Lisboa, Renan T. Figueiredo, Elton Franceschi, Alini T. Fricks, Álvaro S. Lima & Cleide M. F. Soares

  2. Instituto de Tecnologia e Pesquisa, Av. Murilo Dantas, 300, Prédio do ITP, Farolândia, Aracaju, SE, 49032-490, Brazil

    Rebeca Y. Cabrera-Padilla, Renan T. Figueiredo, Elton Franceschi, Alini T. Fricks, Álvaro S. Lima & Cleide M. F. Soares

  3. Departamento de Química, Universidade de Aveiro, Aveiro, Portugal

    Matheus M. Pereira

  4. Departamento de Engenharia de Produção, Universidade Federal de Sergipe, Av. Marechal Rondon, s/n, Jardim Rosa Elze, Sao Cristovao, SE, 49100-000, Brazil

    Daniel P. Silva

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  1. Rebeca Y. Cabrera-Padilla
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  2. Milena C. Lisboa
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  3. Matheus M. Pereira
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  4. Renan T. Figueiredo
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  5. Elton Franceschi
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  6. Alini T. Fricks
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  7. Álvaro S. Lima
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  8. Daniel P. Silva
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  9. Cleide M. F. Soares
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Correspondence to Rebeca Y. Cabrera-Padilla.

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Cabrera-Padilla, R.Y., Lisboa, M.C., Pereira, M.M. et al. Immobilization of Candida rugosa lipase onto an eco-friendly support in the presence of ionic liquid. Bioprocess Biosyst Eng 38, 805–814 (2015). https://doi.org/10.1007/s00449-014-1322-2

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  • DOI: https://doi.org/10.1007/s00449-014-1322-2

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