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Lead Induces Different Responses of Two Subpopulations of Phagocytes in the Holothurian Eupentacta fraudatrix

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Abstract

In view of increasing lead pollution (Pb2+) of coastal waters, the compensatory abilities of holothurians need to be assessed. The goal of the work is to clarify the functional and phenotypical differences between two types of phagocytes (P1 and P2) in Eupentacta fraudatrix exposed to Pb(NO3)2. It has been shown that 2 mg L−1 lead exposure for 48 h increases the number of P2 phagocytes as compared to P1 cells, does not significantly affect cell viability in both P1 and P2 phagocyte fractions, and significantly enhances chromatin condensation in P2 but not in P1 phagocytes. A lead concentration of 4 mg L−1 increases the number of P1 phagocytes compared to that of P2 type, and does not change cell viability and chromatin condensation in P1 phagocytes. In the P2 type, it decreases cell viability and does not influence the level of apoptosis. The protection against lead-induced apoptosis is apparently mediated by the activities of antioxidant enzymes, especially glutathione S-transferase. The differences in labeling cell surface receptors of P1 and P2 phagocytes by plant lectins also indicate the specific phenotypic properties of these cells. The results clarify the potential and GSH-dependent mechanisms of immune adaptation in holothurians that have been shortly exposed to lead at concentrations close to the maximum environmentally relevant level in coastal waters. Additionally, P1 and P2 phagocytes are first shown to have different functions and phenotypes during the response to lead, which indicates the complexity of the phagocytic system in holothurians and contributes to understanding the immunity evolution.

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Acknowledgements

The authors thank the Far Eastern Center of Electron Microscopy, National Scientific Center of Marine Biology, for facilities. They also thank anonymous reviewers for their constructive comments.

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  1. Laboratory of Biochemistry, V.I.Il’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690041, Russia

    Lyudmila S. Dolmatova

  2. Laboratory of Comparative Cytology, National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690041, Russia

    Igor Yu. Dolmatov

  3. Department of Cell Biology and Genetics, Far Eastern Federal University, Vladivostok, 690922, Russia

    Igor Yu. Dolmatov

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Dolmatova, L.S., Dolmatov, I.Y. Lead Induces Different Responses of Two Subpopulations of Phagocytes in the Holothurian Eupentacta fraudatrix. J. Ocean Univ. China 17, 1391–1403 (2018). https://doi.org/10.1007/s11802-018-3795-0

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