Abstract
Genus Grateloupia is one of the most speciose genera in family Halymeniales. It is also one of the sources for natural materials, food and medicine. With different environments, Grateloupia change their morphological characteristics, making their morphological identification very difficult. In addition, few of the species diversity in this genus has been described before. In this study, phylogenetic analysis based on rbcL gene sequence was employed to group Grateloupia collected from three locations along Chinese coast. The microsatellites were also used to evaluate their genetic diversity. In total, the tissue parts of 6 putative species were collected from G. asiatica, G. livida, G. lanceolate, G. catenata, G. turuturu and G. filicina. In order to evaluate their genetic diversity and then conserve them better, 40 microsatellites available for Grateloupia were used to evaluate their genetic diversity, and 11 microsatellites were found to be applicable to determine the genetic diversity of G. asiatica. It was found that the genetic diversity of G. asiatica around Qingdao was very rich. We suggested that the species of genus Grateloupia should be identified based on rbcL phylogenetic analysis before the diversity evaluation with microsatellites. The microsatellites should be developed for each species of Grateloupia so that their genetic diversity can be evaluated appropriately.
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Athukorala, Y, Jung, W. K., Park, P. J., Lee, Y. J., Kim, S. K., Vasanthan, T., No, H. K., and Jeon, Y. J., 2008. Evaluation of biomolecular interactions of sulfated polysaccharide isolated from Grateloupia filicina on blood coagulation factors. Journal of Microbiology & Biotechnology, 18 (3): 503–511.
Baweja, P., and Sahoo, D., 2002. Structure and reproduction of Grateloupia filicina (Halymeniaceae, Rhodophyta) from Indian Coast. Choryu-Hakhoe-chi / The Korean Journal of Phycology, 17 (3): 161–170.
Bellorin, A. M., Amelia, B., Jelveh, S., Oliveira, M. C., and Oliveira, E. C., 2008. Gracilariopsis mclachlanii sp. nov. and Gracilariopsis prseica sp. nov. of the Gracilariaceae (Gracilariales, Rhodophyceae) from the Indian Ocean. Journal of Phycology, 44 (4): 1022–1032.
Botstein, D, White, R. L., Skolnick, M., and Davis, R. W., 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32 (3): 314–331.
Darriba, D., Taboada, G. L., Doallo, R., and Posada, D., 2012. jModelTest 2: More models, new heuristics and high-performance computing. Nature Methods, 9 (8): 772.
Dawczynski, C., Schubert, R., and Jahreis, G., 2007. Amino acids, fatty acids, and dietary fibre in edible seaweed products. Food Chemistry, 103 (3): 891–899.
De Clerck, O., Gavio, B., Fredericq, S., and Barbara, I., 2005. Systematics of Grateloupia filicina (Halymeniaceae, Rhodophyta), based on rbcL sequence analyses and morphological evidence, including the reinstatement of G. minima and the description of G. capensis sp. nov. 1. Journal of Phycology, 41 (2): 391–410.
Faye, E. J., Wang, H. W., Kawaguchi, S., Shimada, S., and Masuda, M., 2004. Reinstatement of Grateloupia subpectinata (Rhodophyta, Halymeniaceae) based on morphology and rbcL sequences. Phycological Research, 52 (1): 59–67.
García-Jiménez, P., Geraldino, P. J. L., Boo, S. M., and Robaina, R. R., 2008. Red alga Grateloupia imbricata (Halymeniaceae), a species introduced into the Canary Islands. Phycological Research, 56 (3): 166–171.
Geraldino, P. J. L., Yang, E. C., Kim, M. S., and Boo, S. M., 2009. Systematics of Hypnea asiatica sp. nov. (Hypneaceae, Rhodophyta) based on morphology and nrDNA SSU, plastid rbcL, and mitochondrial cox1. Taxon, 58 (2): 606–616.
Guindon, S., Dufayard, J. F., Lefort, V., Anisimova, M., Hordijk, W., Gascuel, O., 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: Assessing the performance of PhyML 3.0. Systematic Biology, 59 (3): 307–321.
Hall, T. A., 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41: 95–98.
Hartl, D., and Clark, A., 1997. Principles of Population Genetics. 3rd edition. Sinauer Associates, 143–144.
Kawaguchi, S., Wang, H. W., Horiguchi, T., Lewis, J. A., and Masuda, M., 2002. Rejection of Sinkoraena and transfer of some species of Carpopeltis and Sinkoraena to Polyopes (Rhodophyta, Halymeniaceae). Phycologia, 41 (6): 619–635.
Kawaguchi, S., Wang, H. W., Horiguchi, T., Sartoni, G., and Masuda, M., 2001. A comparative study of the red alga Grateloupia filicina (Halymeniaceae) from the northwestern Pacific and Mediterranean with the description of Grateloupia asiatica. Journal of Phycology, 37 (3): 433–442.
Kim, M. S., 2010. Symphyocladia lithophila sp. nov. (Rhodomelaceae, Ceramiales), a new Korean red algal species based on morphology and rbcL sequences. Botanica Marina, 53 (3): 233–241.
Kim, M. S., Kim, M., Terada, R., Yang, E. C., and Boo, S. M., 2008a. Gracilaria parvispora is the correct name of the species known as G. bursa-pastoris in Korea and Japan. Taxon, 57 (1): 231–237.
Kim, M. S., Yang, E. C., Kim, S. Y., Hwang, I. K., and Boo, S. M., 2008b. Reinstatement of Gracilariopsis chorda (Gracilariaceae, Rhodophyta) based on plastid rbcL and mitochondrial cox1 sequences. Algae, 23 (3): 209–217.
Kim, M. S., Yang, M. Y., and Cho, G. Y., 2010. Applying DNA barcoding to Korean Gracilariaceae (Rhodophyta). Cryptogamie Algologie, 31 (4): 387–401.
Larkin, M. A., Blackshields, G., Brown, N., Chenna, R., Mc-Gettigan, P. A., McWilliam, H., Valentin, F., Wallace, I. M., Wilm, A., Lopez, R., Thompson, J. D., Gibson, T. J., and Higgins, D. G., 2007. Clustal W and Clustal X version 2.0. Bioinformatics, 23 (21): 2947–2948.
Lee, J. I., Kim, H. G., Geraldino, P. J. L., Hwang, I. K., and Boo, S. M., 2009. Molecular classification of the genus Grateloupia (Halymeniaceae, Rhodophyta) in Korea. Medecine Tropicale Revue Du Corps De Sante Colonial, 24 (4): 231–238.
Li, F., Jiang, P., Zhao, S., and Wang, H., 2016. Research on the revision of Grateloupia fastigiata Li et Ding (Rhodophyta, Halymeniaceae) based on morphological observations and rbcL sequence analyses. Acta Hydrobiologica Sinica, 40 (6): 1249–1256 (in Chinese with English abstract).
Lin, S. M., and Hommersand, M., 2008. Two types of auxiliary cell ampullae in Grateloupia (Halymeniaceae, Rhodophyta), including G. taiwanensis sp. nov. and G. orientalis sp. nov. from Taiwan based on rbcL gene sequence analysis and cystocarp development. Journal of Phycology, 44 (1): 196–214.
Liu, M., 2014. Morphological observation and rbcL sequence analysis of a new species from China, Grateloupia boaoensis Wang et Luan sp. nov. (Halymeniaceae, Rhodophyta). Acta Hydrobiologica Sinica, 38 (5): 938–944 (in Chinese with English abstract).
Lu, B., 1994. Does the polymorphism information content equal the heterozygosity? Hereditas, 16 (4): 31–33.
Mateo-Cid, L. E., Mendoza-Gonzalez, A. C., Gavio, B., and Fredericq, S., 2005. Grateloupia huertana sp. nov. (Halymeniaceae, Rhodophyta), a peculiar new prostrate species from tropical Pacific Mexico. Phycologia, 44 (1): 4–16.
Nei, M., Maruyama, T., and Chakraborty, R., 1975. The bottleneck effect and genetic variability in populations. Evolution, 29 (1): 1–10.
Ronquist, F., and Huelsenbeck, J. P., 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19 (12): 1572–1574.
Saunders, G. W., 2008. A DNA barcode examination of the red algal family dumontiaceae in Canadian waters reveals substantial cryptic species diversity. 1. The foliose Dilsea-Neodilsea complex and Weeksia. Botany, 86 (7): 773–789.
Swofford, D., 2002. PAUP* 4.0: Phylogenetic Analysis Using Parsimony. Sinauer Associates.
Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S., 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology & Evolution, 30 (12): 2725.
Wang, H., 2000. Reinstatement of Grateloupia catenata (Rhodophyta, Halymeniaceae) on the basis of morphology and rbcL sequences. Phycologia, 39 (3): 228–237.
Wang, J., Peng, C., Liu, Z. Y., Tang, Z. H., and Yang, G. P., 2013. Isolation and characterization of microsatellites of Grateloupia filicina. Conservation Genetics Resources, 5 (3): 763–766.
Wang, S. C., Bligh, S. W., Shi, S. S., Wang, Z. T., Hu, Z. B., Crowder, J., Branford-White, C., and Vella, C., 2007. Structural features and anti-HIV-1 activity of novel polysaccharides from red algae Grateloupia longifolia and Grateloupia filicina. International Journal of Biological Macromolecules, 41 (4): 369–375.
Wilkes, R. J., McIvor, L. M., and Guiry, M. D., 2005. Using rbcL sequence data to reassess the taxonomic position of some Grateloupia and Dermocorynus species (Halymeniaceae, Rhodophyta) from the north-eastern Atlantic. European Journal of Phycology, 40 (1): 53–60.
Wong, S. L., and Chang, J., 2000. Salinity and light effects on growth, photosynthesis, and respiration of Grateloupia filicina (Rhodophyta). Aquaculture, 182 (3): 387–395.
Xia, B. M., 2004. Flora algarum marinarum sinicarum Tomus II Rhodophyta No. III Gelidiales Cryptonemiales Hildenbrandiales. Science Press, Beijing, 1–19 (in Chinese).
Xiong, M., Shi, F., Xu, L., Que, Y., Yang, Z., Hu, J., and Zhu, B., 2011. Isolation and characterization of microsatellite loci in Spinibarbus sinensis Bleeker. Journal of Hydroecology, 32 (1): 40–44.
Ye, M., 2011. Isolation of microsatellite markers from Charybdis japonica and its application in genetic diversity analysis. Master thesis. Zhejiang Ocean University (in Chinese).
Yeh, F., Yang, R., Boyle, T., Ye, Z., and Xiyan, J., 2000. POPGENE 32, Microsoft Windows-Based Freeware for Population Genetic Analysis. Molecular Biology and Biotechnology Center. University of Alberta, Edmonton.
Zablackis, E., and Perez, J., 1990. A partially pyruvated carrageenan from Hawaiian Grateloupia filicina (Cryptonemiales, Rhodophyta). Botanica Marina, 33 (3): 273–276.
Zhang, W., Wang, H. W., Zhao, D., Sheng, Y. W., and Luan, R. X., 2012. Morphological observation and rbcL gene sequence analysis of Grateloupia orientalis (Rhodophyta), a new record of the Hainan Province. Marine Sciences, 36 (7): 109–116 (in Chinese with English abstract).
Zhao, D., Wang, H., Sheng, Y., Lu, J., and Luan, R., 2012. Morphological observation and rbcL gene sequences studies of two new species, Grateloupia dalianensis H.W.Wang et D.Zhao, sp. nov. and G. yinggehaiensis H.W.Wang et R.X. Luan, sp. nov. (Halymeniaceae, Rhodophyta) from China. Nature Methods, 31 (2): 109–120.
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This work was supported by the Marine Industry Research Special Funds for Public Welfare Projects (No. 201205024).
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Peng, C., Ding, H., Tang, Z. et al. Molecular Grouping of Grateloupia Tissues Collected Along Chinese Coast and Microsatellite Diversity Analysis of G. asiatica. J. Ocean Univ. China 17, 925–931 (2018). https://doi.org/10.1007/s11802-018-3496-8
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DOI: https://doi.org/10.1007/s11802-018-3496-8