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Isolation and characterization of microsatellites of Grateloupia filicina

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Abstract

Grateloupia filicina has long been considered a textbook example of a marine red alga with a cosmopolitan distribution. It is of important ecological, edible and medicinal values. We isolated 40 new microsatellite DNAs of G. filicina with a modified fast isolation by AFLP of sequences containing repeat protocol and characterized them in 56 individuals collected from Qingdao coast. The number of alleles per locus ranged from 3 to 10. The observed and expected heterozygosities varied between 0.11 and 0.72 and 0.20 and 0.93, respectively. Ten loci deviated from Hardy–Weinberg equilibrium and two loci were at linkage disequilibrium. The microsatellites developed in this study will certainly facilitate the researches on the genetic diversity and differentiation of G. filicina populations.

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Acknowledgments

This work was supported by a special found for marine industries with public interests (201205024).We are grateful to colleagues from Shandong Oriental Ocean Sci-Tech Co. Ltd, Linan Zhang, Xin Jiang for assistance with data processing and revision.

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Authors and Affiliations

  1. Key Laboratory of Marine Genetics and Breeding of Chinese Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, People’s Republic of China

    Jun Wang, Chong Peng, Zhenyu Liu, Zhihong Tang & Guanpin Yang

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  1. Jun Wang
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  2. Chong Peng
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  3. Zhenyu Liu
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  4. Zhihong Tang
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  5. Guanpin Yang
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Correspondence to Guanpin Yang.

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Wang, J., Peng, C., Liu, Z. et al. Isolation and characterization of microsatellites of Grateloupia filicina . Conservation Genet Resour 5, 763–766 (2013). https://doi.org/10.1007/s12686-013-9902-z

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  • DOI: https://doi.org/10.1007/s12686-013-9902-z

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