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Analysis of DNA Methylation of Gracilariopsis lemaneiformis Under Temperature Stress Using the Methylation Sensitive Amplification Polymorphism (MSAP) Technique

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Abstract

Gracilariopsis lemaneiformis is an economically important agarophyte, which contains high quality gel and shows a high growth rate. Wild population of G. lemaneiformis displayed resident divergence, though with a low genetic diversity as was revealed by amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) analyses. In addition, different strains of G. lemaneiformis are diverse in morphology. The highly inconsistence between genetic background and physiological characteristics recommends strongly to the regulation at epigenetic level. In this study, the DNA methylation change in G. lemaneiformis among different generation branches and under different temperature stresses was assessed using methylation sensitive amplified polymorphism (MSAP) technique. It was shown that DNA methylation level among different generation branches was diverse. The full and total methylated DNA level was the lowest in the second generation branch and the highest in the third generation. The total methylation level was 61.11%, 60.88% and 64.12% at 15°C, 22°C and 26°C, respectively. Compared with the control group (22°C), the fully methylated and totally methylated ratios were increased in both experiment groups (15°C and 26°C). All of the cytosine methylation/demethylation transform (CMDT) was further analyzed. High temperature treatment could induce more CMDT than low temperature treatment did.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31372529), the Fundamental Research Funds for the Central Universities (No. 201762016) and China Agriculture Research System (No. CARS-50).

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Authors and Affiliations

  1. Key Laboratory of Marine Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao, 266003, China

    Chong Peng, Zhenghong Sui, Wei Zhou, Yiyi Hu, Ping Mi, Minjie Jiang, Xiaodong Li & Xudong Ruan

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  1. Chong Peng
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  2. Zhenghong Sui
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  3. Wei Zhou
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  4. Yiyi Hu
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Correspondence to Zhenghong Sui.

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Peng, C., Sui, Z., Zhou, W. et al. Analysis of DNA Methylation of Gracilariopsis lemaneiformis Under Temperature Stress Using the Methylation Sensitive Amplification Polymorphism (MSAP) Technique. J. Ocean Univ. China 17, 623–631 (2018). https://doi.org/10.1007/s11802-018-3426-9

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