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Microwave-assisted rapid synthesis of ovalbumin-stabilized gold nanoclusters for picric acid determination

微波辅助快速合成卵清蛋白金纳米簇检测苦味酸

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Abstract

In this work, ovalbumin-stabilized gold nanoclusters (OVA-Au NCs) fluorescent nanoprobes were synthesized by microwave heating and applied to detect picric acid (PA). The nanoprobes emitted red fluorescence with the maximum fluorescence peak of 680 nm under the excitation wavelength of 350 nm, and the Stokes shifts could be up to 330 nm which could effectively eliminate the interference of resonance scattering light. Compared with hydrothermal method, the synthesis method was simple and fast, and only took 50 s. Due to the absorption peak of PA overlapped with the emission peak of OVA-Au NCs in a large range, PA could selectively quench the fluorescence of OVA-Au NCs based on the inner filter effect (IFE) and a quick response time (1 min). Therefore, a new and sensitive method for PA monitoring was established. Under the optimal conditions, the concentration of PA demonstrated a satisfactory linear correlation with the fluorescence quenching degree ΔF/F0 of the sensing system in the range of 20–240 µmol/L with the detection limit of 6.4 µmol/L. The proposed method is simple, fast, accurate, and easy to realize real-time monitoring.

摘要

本文采用微波加热法快速合成了卵清蛋白金纳米簇荧光探针(OVA-Au NCs)并应用于检测苦味酸 (PA)。该探针在350 nm 波长激发下,能发射红色荧光,最大发射荧光峰为680 nm,Stokes 位移高达 330 nm, 可有效消除共振散射光的干扰。该合成方法与水热法相比,步骤简单快速,仅用50 s 即可完 成。由于PA的吸收光谱与OVA-Au NCs的激发光谱有较大范围的重叠,基于内滤效应(IFE) PA可选择 性猝灭OVA-Au NCs的荧光,据此建立了检测苦味酸的新方法。在最佳实验条件下,在20~240 μmol/L 范围内,检测体系的荧光猝灭效率ΔF/F0与PA的浓度具有良好的线性关系,r 为0.9985,检测限为 6.4 μmol/L。该方法简便、快速、准确,易于实现实时监测。

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Author notes

  1. FAN Peng-fei and LIU Can contributed equally to this work

Authors and Affiliations

  1. Department of Public Health Laboratory Sciences, School of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China

    Peng-fei Fan  (樊鹏飞), Can Liu  (刘璨), Qian-ji Li  (李千纪), Cong-cong Hu  (胡聪聪), Xi-wen Wu  (吴禧雯), Xiao-huan Zhang  (张晓环), Hao Liang  (梁好) & Sheng-yuan Yang  (杨胜园)

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  1. Peng-fei Fan  (樊鹏飞)
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Contributions

FAN Peng-fei and LIU Can provided and analyzed the measured data, and established the method. LI Qian-ji and HU Cong-cong validated the proposed method with practical experiments. WU Xi-wen and ZHANG Xiao-huan analyzed the calculated results. The initial draft of the manuscript was written by FAN Peng-fei and LIU Can. YANG Sheng-yuan and LIANG Hao edited the manuscript and provided projects support. All authors replied to reviewers’ comments and revised the final version.

Corresponding authors

Correspondence to Hao Liang  (梁好) or Sheng-yuan Yang  (杨胜园).

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Conflict of interest

FAN Peng-fei, LIU Can, LI Qian-ji, HU Cong-cong, WU Xi-wen, ZHANG Xiao-huan, LIANG Hao and YANG Sheng-yuan declare that they have no conflict of interest.

Foundation item: Projects(82073604, 81903369) supported by the National Natural Science Foundation of China; Project (S202010555075) supported by the National Innovation Training Program for College Students, China; Project (CX20210962) supported by the Postgraduate Research and Innovation Project of Hunan Province, China

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Fan, Pf., Liu, C., Li, Qj. et al. Microwave-assisted rapid synthesis of ovalbumin-stabilized gold nanoclusters for picric acid determination. J. Cent. South Univ. 30, 74–84 (2023). https://doi.org/10.1007/s11771-023-5224-9

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  • DOI: https://doi.org/10.1007/s11771-023-5224-9

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