Abstract
The metal tolerant plant growth-promoting bacteria (MT-PGPB) adapt different strategies to cope up with the metal stressed soil and improve the growth of plants in such areas. The present study was therefore, designed to isolate MT-PGPBs from the coal dumping area of Dudhnoi, Goalpara, Assam, India. In this study, a total of 10 bacterial strains were obtained from the coal dumping area and 21 from reference forest soil using nutrient agar media. Among them, four bacterial strains (Bacillus toyonensis DD1, B. mycoides DD2, B. velezensis DD9 and B. flexus DD10) obtained from the coal dumping area could tolerate two or more heavy metals (Zn, Fe, Pb, Ni, Cu, Cd, Cr). Besides, these metal tolerating bacterial isolates were evaluated for their plant growth-promoting activities like production of indole-3-acetic acid, siderophore, ACC deaminase activity, and phosphate solubilization. The results showed that three isolates were capable of solubilizing phosphate, produced IAA, and showed ACC deaminase activity, while all 4 isolates produced siderophore. Hence, these four bacterial isolates might help in microbe assisted phytoremediation in the future.
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Abbreviations
- MT-PGPB:
-
Metal tolerant plant growth-promoting bacteria
- FE-SEM:
-
Field emission scanning electron microscopy
- MC:
-
Moisture content
- TOC:
-
Total organic carbon
- TN:
-
Total nitrogen
- AvP:
-
Available phosphorus
- AvK:
-
Available potassium
- AAS:
-
Atomic absorption spectrophotometry
- NA:
-
Nutrient agar
- CFU:
-
Colony forming unit
- MIC:
-
Minimum inhibitory concentration
- IAA:
-
Indole-3-Acetic Acid
- ACC:
-
Deaminase, 1-aminocyclopropane-1-carboxylate deaminase
- CAS:
-
Chrome azurol S
- LB:
-
Luria-Bertani
- CTAB:
-
Cetyl trimethylammonium bromide
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Acknowledgments
The authors are highly thankful to the Head, Department of Botany, Gauhati University, Guwahati for providing the required instrumentation facilities under UGC-SAP and DST-FIST. The first author gratefully acknowledges the financial support provided by the Department of Biotechnology, Govt. of India under the DBT-RA Program in Biotechnology and Life Sciences.
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This study was partially supported by the Department of Biotechnology, Government of India under the DBT-RA Program in Biotechnology and Life Sciences. The funders had no role in study design, data collection, and analysis, decision to publish, or preparation of the manuscript.
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DB and DKJ designed the research. DB and ID performed the experiments. DB and DKJ analyzed the data. DB drafted the manuscript. DB and DKJ finalized the manuscript. All authors read and critically reviewed the manuscript.
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Barman, D., Dutta, I. & Jha, D.K. Heavy metal resistant bacteria from coal dumping site with plant growth promoting potentials. Biologia 77, 533–545 (2022). https://doi.org/10.1007/s11756-021-00963-y
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DOI: https://doi.org/10.1007/s11756-021-00963-y