Abstract
A novel strain of the genus Micrococcus isolated from wastewater was studied for resistance to seven heavy metals and forty antibiotics. Its capacity to accumulate metal ions was also realized at different pH. The strain exhibited high minimal inhibitory concentration values for metal ions tested and resist to 15 antibiotics. The living cells of the bacterial strain show a largest uptake capacity at pH 6–8.5 for copper, nickel, and zinc with values ranging from 51.45 to 83.90 %, 52.59 to 78.81 %, and 59.55 to 78.90 %, respectively. It was also able to absorbed 59.81–80.08 % of chromium and 58.09–79.41 % of cobalt at pH 7.3–8.5. The maximum lead uptake was obtained at pH 5.5–8.5 with an amount of 55.28–91.06 %. The significant absorption of cadmium was shown at pH 6.5 with 38 %. In 25 µg mL-1 zinc, chromium, and nickel solutions, dead cells of the isolate were able to biosorbed 20.46, 22.5, and 23.98 µg mL−1, respectively, after 30 min of contact. In other solutions with higher concentrations 50 and 100 µg mL−1, the amount of each metal immobilized was, respectively, as follows: 38.02 and 90.21 µg mL−1 for zinc, 39.78 and 89.23 µg mL−1 for chromium, and 47.19 and 86.83 µg mL−1 for nickel. Due to its high-metal accumulation capacity in aerobic conditions, these Gram-positive bacteria may be potentially applicable in situ bioremediation of heavy metals contaminating aqueous systems.
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Acknowledgments
We are thankful to the Police Scientific Laboratory of Algiers and the Laboratory of Microbiology, IRD, France, for their help. We also like to thank Microbiology Laboratory Staff of the FSB/USTHB, Algiers, Algeria.
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Benmalek, Y., Fardeau, ML. Isolation and characterization of metal-resistant bacterial strain from wastewater and evaluation of its capacity in metal-ions removal using living and dry bacterial cells. Int. J. Environ. Sci. Technol. 13, 2153–2162 (2016). https://doi.org/10.1007/s13762-016-1048-6
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DOI: https://doi.org/10.1007/s13762-016-1048-6