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Development of male and female gametophytes and embryogenesis in the Arabidopsis thaliana

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Abstract

Sporogenesis and gametophytes development in Arabidopsis thaliana (Brassicaceae) was studied. Arabidopsis thaliana was selected for this study as a biogenetic model with a small genome and a short life cycle. Flowers and buds in different developmental stages were removed, fixed in an FAA70, stored in 70 % ethanol, embedded in paraffin and sectioned at 7 − 10 µm with a microtome, stained with Hematoxylin – Eosin and analyzed with a photomicroscope. The results showed the anthers are tetrasporangiate, anther tapetum is of secretory type at the beginning and plasmodial at the end of anther development. Also, microspore tetrads are tetrahedral and rarely tetragonal. In addition, mature pollen grains are spherical, tricolporate, scabrate, and tricellular. The gynoecium is bicarpellate and the ovule is amphitropous, bitegmic, and tenuinucellate. Hypostasis was also observed in basis of nucellus. Endothelium is composed of one cell row and cell divisions of megaspore mother cell results in T-shaped tetrad. The chalazal cell is a functional megaspore that survives and functions in megagametophyte development. The embryo sac development belonged to the Polygonum type and it is curved. The mature embryo sac is composed of 7 cells, one central cell containing polar nuclei, two elongated synergids, a triangular oosphere cell, and three antipodal cells that are degenerated immediately by developing the embryogenesis stage in the mature embryo.

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References

  • Aghajafari M, Behboodi BS, Pirayesh S (2013) Study on the morphology of genus Arabidopsis in Iran. Am J Plant Sci 2013. https://doi.org/10.4236/ajps.2013.412A1004

  • Al-shehbaz I (2003) Transfer of most North American species of Arabis to Boechera (Brassicaceae). Missouri Botanical Garden Press 13, No. 4, Winter, 2003, pp 381–391. https://doi.org/10.2307/3393366

  • Belyaeva L, Rodionova G (1983) Family Brassicaceae comparative embryology of flowering plants. Phytolaccaceae—Thymelaeceae 3:154–164

    Google Scholar 

  • Bouman F (1974) Developmental studies of the ovule, integuments, and seed in some angiosperms. Thesis University Amsterdam

  • Bouman F (1975) Integument initiation and testa development in some Cruciferae. Bot J Linn Soc 70:213–229. https://doi.org/10.1111/j.1095-8339.1975.tb01646

  • Chehregani A, Mahanfar N (2007) Achene micro-morphology of Anthemis (Asteraceae) and its allies in Iran with emphasis on systematics International. J Agric Biol 9:486–488

    Google Scholar 

  • Chehregani A, Sedaghat M (2009) Pollen grain and ovule development in Lepidium vesicarium (Brassicaceae). Int J Agric Biol 11:1560–8530

    Google Scholar 

  • Chehregani A, Tanomi N (2010) Ovule ontogenesis and megagametophyte development in Onobrychis schahuensis Bornm.(Fabaceae). Turk J Bot 34:241–248. https://doi.org/10.3906/bot-0908-168

    Article  Google Scholar 

  • Davis GL (1962) Embryological studies in the compositae, I. sporogenesis, gametogenesis, and embryogeny in Cotula australis (less.) hook F. Aust J Bot 10:1–12. https://doi.org/10.1071/BT9620001

    Article  Google Scholar 

  • Davis G (1966) Systematic embryology f the angiosperms. Willey, Hoboken

    Google Scholar 

  • Davis G (1966) Systematic embryology of angiosperms. Wiley, New York

    Google Scholar 

  • Diboll AG (1968) Fine structural development of the megagametophyte of Zea mays following fertilization American. J Bot 55:787–806. https://doi.org/10.2307/2440968

    Article  Google Scholar 

  • Ghimire B, Heo K (2012) Embryology of Withania somnifera (L.) dunal (Solanaceae). Acta Biol Cracov Ser Bot 54:69–78

    Google Scholar 

  • Johansson M, Walles B (1993) Functional anatomy of the ovule in broad bean (Vicia faba L.). I. Histogenesis prior to and after pollination International. J Plant Sci 154:80–89

    Article  Google Scholar 

  • Johri B, Ambegaokar K, Srivastava P (1992) Comparative embryology of angiosperms, vol. 1. Berlin, Heidelberg Springer Berlin Heidelberg

  • Jürgens G, Mayer U, Berleth T, Miséra S (1991) Genetic analysis of pattern formation in the Arabidopsis embryo. Development 113:27–38

    Google Scholar 

  • Kai-yu P, Jie W, Shi-liang Z (1997) Embryological study on Mosla chinensis (Lamiaceae). Acta Bot Sin 39:111–116

  • Kasahara RD, Maruyama D, Hamamura Y, Sakakibara T, Twell D, Higashiyama T (2012) Fertilization recovery after defective sperm cell release in Arabidopsis. Curr Biol 22:1084–1089. https://doi.org/10.1016/j.cub.2012.03.069

    Article  CAS  PubMed  Google Scholar 

  • Khan R (2004) Studies on the pollen morphology of the genus Arabidopsis (Brassicaceae) from Pakistan . Pak J Bot 36:229–234

    Google Scholar 

  • Koch MA (2019) The plant model system Arabidopsis set in an evolutionary, systematic, and spatio-temporal context. J Exp Bot 70:55–67. https://doi.org/10.1093/jxb/ery340

    Article  CAS  PubMed  Google Scholar 

  • Koch MA, Matschinger M (2007) Evolution and genetic differentiation among relatives of Arabidopsis thaliana. Proc Natl Acad Sci 104:6272–6277. https://doi.org/10.1073/pnas.0701338104

  • Koch M, Haubold B, Mitchell-Olds T (2001) Molecular systematics of the Brassicaceae: evidence from coding plastidic matK and nuclear Chs sequences. Am J Bot 88:534–544

    Article  CAS  Google Scholar 

  • O’Kane SL Jr, Al-Shehbaz IA (1997) A synopsis of Arabidopsis (Brassicaceae). Novon :323–327. https://doi.org/10.2307/3391949

  • Pacini E, Franchi G, Hesse M (1985) The tapetum: Its form, function, and possible phylogeny inEmbryophyta. Plant Syst Evol 149:155–185

    Article  Google Scholar 

  • Page DR, Grossniklaus U (2002) The art and design of genetic screens: Arabidopsis thaliana. Nat Rev Genet 3:124–136. https://doi.org/10.1038/nrg730

    Article  CAS  PubMed  Google Scholar 

  • Poddubnaya-Arnol’di VA (1982) Characteristics of angiosperm families in relation to cytoembryological characters. Nauka, Moscow

  • Prakash N (1987) Embryology of the leguminosae. In: Stirton CH (ed) Advances in legume systematics, 241–278, Part 3. Royal Botanical Gardens, Kew, England

  • Prasad K (1977) The development and structure of basal body in the ovule and seed of certain species of Cruciferae. Bot Jahrb Syst 98:266–272

    Google Scholar 

  • Preuss D, Rhee SY, Davis RW (1994) Tetrad analysis possible in Arabidopsis with mutation of the QUARTET (QRT) genes. Science 264:1458–1460. https://doi.org/10.1126/science.8197459

    Article  CAS  PubMed  Google Scholar 

  • Ramezani H, Chehregani Rad A, Karamian R (2018) Development of the male and female gametophyte in Capsicum annuum L. var. California Wonder . Plant Biosyst 152:1172–1181. https://doi.org/10.1080/11263504.2018.1435568

    Article  Google Scholar 

  • Rao CV (1963) Studies in the Proteaceae. 3. Tribe Oriteae Proceedings of the National Institute of Sciences of India. Calcutta 29:489–510

  • Rédei GP (1975) Arabidopsis as a genetic tool Annual review of genetics 9:111–127. https://doi.org/10.1146/annurev.ge.09.120175.000551

  • Rembert JDH (1969) Comparative megasporogenesis in Caesalpiniaceae. Bot Gaz 130:47–52

    Article  Google Scholar 

  • Rembert JDH (1969) Comparative megasporogenesis in Papilionaceae. Am J Bot 56:584–591

    Article  Google Scholar 

  • Rembert JDH (1971) Phylogenetic significance of megaspore tetrad patterns in Leguminales. Phytomorphology 21:317–416

    Google Scholar 

  • Rembert Junior DH (1977) Contribution to ovule ontogeny in Glycine max. Phytomorphology 27:368–370

    Google Scholar 

  • Rollins RC (1941) Monographic study of Arabis in western North America Contributions from the Gray Herbarium of Harvard University:289–485. https://digitalcommons.usu.edu/etd

  • Scott RJ, Spielman M, Bailey J, Dickinson HG (1998) Parent-of-origin effects on seed development in Arabidopsis thaliana. Development 125:3329–3341

    CAS  PubMed  Google Scholar 

  • Shamrov I (2002) Ovule and seed morphogenesis in Capsella bursapastoris (Brassicaceae) in connection with peculiar mode of endothelium formation . Botanicheskij Zhurnal (St Petersburg) 87:1–18

    Google Scholar 

  • Somssich M (2018) A short history of Arabidopsis thaliana (L.) Heynh. Columbia-0 (No. e26931v2). PeerJ Preprints, Australia. https://doi.org/10.7287/peerj.preprints.26931v3

  • Sun K, Hunt K, Hauser BA (2004) Ovule abortion in Arabidopsis triggered by stress. Plant Physiol 135:2358–2367. https://doi.org/10.1104/pp.104.043091

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Twell D, Park SK, Lalanne E (1998) Asymmetric division and cell-fate determination in developing pollen. Trends Plant Sci 3:305–310. https://doi.org/10.1016/S1360-1385(98)01277-1

    Article  Google Scholar 

  • Vaughan JG, Whitehouse JM (1971) Seed structure and the taxonomy of the Cruciferae. Bot J Linn Soc 64:383–409. https://doi.org/10.1111/j.1095-8339.1971.tb02153

  • Vijayabaghavan M, Prabhakar K (1981) Ontogenetical and histochemical studies on chalazal proliferating tissue in Iberis amara and Alyssum maritimum. Beitrage zur Biologie der Pflanzen 56(1):7–17

  • Xue C-Y, Li D-Z (2005) Embryology of Megacodon stylophorus and Veratrilla baillonii (Gentianaceae): descriptions and systematic implications. Bot J Linn Soc 147:317–331

    Article  Google Scholar 

  • Yadegari R, Drews GN (2004) Female gametophyte development. Plant Cell 16:S133–S141. https://doi.org/10.1105/tpc.018192

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yankova E (2004) Comparative-embryological study on the bulgarian representatives of grnus Angelica L. (Apiaceae) and revealing the possibility of their in vitro cultivation. Phd Thesis. Sofia(1) (PDF) On the reproductive biology of Angelica archangelica L. (Apiaceae). https://www.researchgate.net/publication/275040581_On_the_reproductive_biology_of_Angelica_archangelica_L_Apiaceae. Accessed 03 Feb 2021

  • Yankova-Tsvetkova E, Yurukova-Grancharova P, Vladimirov V (2016) On the embryology of Brassica jordanoffii (Brassicaceae)–an endemic species in the Bulgarian flora Phytologia Balcanica. Int J Balkan Flora Veg 22:149–153

    Google Scholar 

  • Yeung EC (1998) A beginner’s guide to the study of plant structure. In: Karcher SJ (ed) Tested studies for laboratory teaching, Vol. 19. Proceedings of the 19th Workshop/Conference of the Association for Biology Laboratory Education (ABLE), pp, 125–141. Purdue University, Lafayette, Indiana. https://doi.org/10.1007/1-4020-2985-3_41

  • Yeung E, Cavey M (1990) Developmental changes in the inner epidermis of the bean seed coat. Protoplasma 154:45–52. https://doi.org/10.1006/anbo.1994.1114

    Article  Google Scholar 

  • Yurukova-Grancharova P, Anchev M, Goranova V (2004) Embryological study on diploid and triploid populations in Iberis saxatilis subsp. saxatilis (Brassicaceae) in Bulgarian flora. Phytologia Balcan 10(1):69–78

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Acknowledgements

This study was supported by the Research and Technology chronicle of Biology Department, College of Bioscience, Islamic Azad University, Tehran North Branch, Tehran, Iran, and the Department of Plant Biotechnology, National Institute for Genetic Engineering and Biotechnology.

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Authors and Affiliations

  1. Biology Department, College of Bioscience, Islamic Azad University, Tehran North Branch, Tehran, Iran

    Elham Basiri, Sayeh Jafari Marandi, Sedighe Arbabian & Ahmad Majd

  2. Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

    Mohammad Ali Malboobi

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  1. Elham Basiri
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Basiri, E., Jafari Marandi, S., Arbabian, S. et al. Development of male and female gametophytes and embryogenesis in the Arabidopsis thaliana. Biologia 76, 853–863 (2021). https://doi.org/10.1007/s11756-021-00682-4

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