Abstract
Reaction conditions for the synthesis of monoglycerides (MG) by enzyme-catalyzed glycerolysis of rapeseed oil using Lipozyme® IM have been studied. Silica gel was used to adsorb the glycerol to overcome the problems of low glycerol solubility in the organic phase. An experimental design was used where temperature, time, the ratio of silica gel to glycerol (w/w), the water activity (a) w , the isooctane concentration, and the ratio of glycerol to triglycerides (mol/mol) were varied. Response surface methodology was used to evaluate initial reaction rate and yield for the different products. The best yield of MG achieved under the studied conditions was 17.4% (mol fatty acid in substance/total mol fatty acid in mixture) (75°C, 20 h, silica gel/glycerol 2:1, a w =0.17, 48% isooctane, glycerol/triglycerides 6:1). The same conditions yielded 36.8% diglycerides, 13.6% free fatty acids (FFA), and 36.9% triglycerides. This is at the same level as the equilibrium yield. The yield of MG is low compared to the final yield achieved with solid-phase glycerolysis. However, in solid-phase glycerolysis the reaction mixture becomes solid, and therefore the solid-phase process is not suitable for industrial application. The formation of FFA was very fast compared to the synthesis of MG. Equilibrium for FFA was reached within 2 h, and the yield was strongly affected by the a w . Increasing a w greatly increased the formation of FFA. In the a w ratio 0.06–0.3, the yield of FFA increased from 4 to 19% while the yield of MG was nearly unaffected. As FFA is an undesired product, it is important to keep the a w as low as possible.
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References
Bornscheuer, U.T., Lipase-Catalyzed Syntheses of Monoacylglycerols, Enzyme Microb. Technol. 17:578–586 (1995).
Krog, N.J., Food Emulsifiers and Their Chemical and Physical Properties, in Food Emulsions, edited by K. Larsson and S.E. Friberg, Marcel Dekker Inc., New York, 1990, pp. 127–180.
Sonntag, N.O.V., Glycerolysis of Fats and Methyl Esters—Status, Review and Critique, J. Am. Oil Chem. Soc. 59:795A-802A (1982).
Henry, C., Monoglycerides: The Universal Emulsifier, Cereal Foods World 40:734–738 (1995).
Berger, M., and M.P. Schneider, Enzymatic Esterification of Glycerol II. Lipase-Catalyzed Synthesis of Regioisomerically Pure 1(3)-rac-Monoacylglycerols, J. Am. Oil Chem. Soc. 69:961–965 (1992).
Hayes, D.G., and E. Gulari, 1-Monoglyceride Production from Lipase-Catalyzed Esterification of Glycerol and Fatty Acid in Reverse Micelles, Biotechnol. Bioeng. 38:507–517 (1991).
Akoh, C.C., C. Cooper, and C.V. Nwosu, Lipase G-Catalyzed Synthesis of Monoglycerides in Organic Solvent and Analysis by HPLC, J. Am. Oil Chem. Soc. 69:257–260 (1992).
Akoh, C.C., Lipase-Catalyzed Synthesis of Partial Glyceride, Biotechnol. Lett. 15:949–954 (1993).
Holmberg, K., and E. Österberg, Enzymatic Preparation of Monoglycerides in Microemulsion, J. Am. Oil Chem. Soc. 65:1544–1548 (1988).
Ferreira-Dias, S., and M.M.R. da Fonseca, Production of Monoglycerides by Glycerolysis of Olive Oil with Immobilized Lipases: Effect of the Water Activity, Bioprocess Eng. 12:327–337 (1995).
McNeill, G.P., S. Shimizu, and T. Yamane, Solid Phase Enzymatic Glycerolysis of Beef Tallow Resulting in a High Yield of Monoglyceride, J. Am. Oil Chem. Soc. 67:779–783 (1990).
McNeill, G.P., S. Shimizu, and T. Yamane, High-Yield Enzymatic Glycerolysis of Fats and Oils, Ibid.1–5 (1991).
Stevenson, D.E., R.A. Stanley, and R.H. Furneaux, Glycerolysis of Tallow with Immobilised Lipase, Biotechnol. Lett. 15:1043–1048 (1993).
Ota, Y., T. Takasugi, and M. Suzuki, Synthesis of Either Mono- or Diacylglycerol from High-Oleic Sunflower Oil by Lipase-Catalyzed Glycerolysis, Food Sci. Technol. Int. Tokyo 3:384–387 (1997).
Chang, P.S., J.S. Rhee, and J.-J. Kim, Continuous Glycerolysis of Olive Oil by Chromobacterium viscosum Lipase Immobilized in Liposome in Reversed Micelles, Biotechnol. Bioeng. 38:1159–1165 (1991).
Castillo, E., V. Dossat, A. Marty, J.S. Condoret, and D. Combes, The Role of Silica Gel in Lipase-Catalyzed Esterification Reactions of High-Polar Substrates, J. Am. Oil Chem. Soc. 74:77–85 (1997).
Halling, P.J., Salt Hydrates for Water Activity Control with Biocatalysts in Organic Media, Biotechnol. Tech. 6:271–276 (1992).
Halling, P.J., Thermodynamic Predictions for Biocatalysis in Nonconventional Media: Theory, Tests, and Recommendations for Experimental Design and Analysis, Enzyme Microb. Technol. 16:178–206 (1994).
Naesens, W., G. Bresseleers, and P. Tobback, Diffusional Behaviour of Tripalmitin in a Freeze-Dried Model System at Different Water Activities, J. Food Sci. 47:1245–1249 (1982).
Goderis, H.L., G. Ampe, M.P. Feyten, B.L. Fouwé, W.M. Guffens, S.M. Van Cauwenbergh, and P.P. Tobback, Lipase-Catalyzed Ester Exchange Reactions in Organic Media with Controlled Humidity, Biotechnol. Bioeng. 30:258–266 (1987).
Stevenson, D.E., R.A. Stanley, and R.H. Furneaux, Near-Quantitative Production of Fatty Acid Alkyl Esters by Lipase-Catalyzed Alcoholysis of Fats and Oils with Adsorption of Glycerol by Silica Gel, Enzyme Microb. Technol. 16:478–484 (1994).
Box, G., W.G. Hunter, and J.S. Hunter, Statistics for Experiments. An Introduction to Design, Data Analysis and Model Building, John Wiley & Sons, New York, 1978.
Rockland, L.B., Saturated Salt Solutions for Static Control of Relative Humidity Between 5° and 40°C, Anal. Chem. 32:1375–1376 (1960).
Greenspan, L., Humidity Fixed Points of Binary Saturated Aqueous Solutions, J. Res. Nat. Bur. Stand. 81A:89–96 (1977).
Elfman-Börjesson, I., and M. Härröd, Analysis of Non-Polar Lipids by HPLC on a Diol Column, J. High Resolut. Chromatogr. 20:516–518 (1997).
Gupta, M., in Bailey’s Industrial Oil & Fat Products, Volume 4, Edible Oil & Fat Products: Processing Technology, 5th edn., edited by Y.H. Hui, John Wiley & Sons, Inc., New York, 1996, pp. 569–601.
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Elfman-Börjesson, I., Härröd, M. Synthesis of monoglycerides by glycerolysis of rapeseed oil using immobilized lipase. J Amer Oil Chem Soc 76, 701–707 (1999). https://doi.org/10.1007/s11746-999-0162-8
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DOI: https://doi.org/10.1007/s11746-999-0162-8