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Enzymatic preparation of monoglycerides in microemulsion

  • Surfactants & Detergents Technical
  • Published:
Journal of the American Oil Chemists’ Society

Abstract

Monoglycerides have been obtained in 80% yield by enzyme catalyzed hydrolysis of the corresponding triglyceride. The reaction was carried out in an oil-rich microemulsion (L 2 phase) formulated without cosurfactant. Best results were obtained with the anionic surfactant sodium bis(2-ethylhexyl)sulfosuccinate (AOT), isooctane as hydrocarbon component and a molar ratio of aqueous buffer of pH 7 to surfactant of 12. The enzyme used was a 1,3-specific lipase which leaves the 2- position intact. However, the 2-monoglyceride formed slowly undergoes acyl migration to 1-monoglyceride which subsequently is hydrolyzed to glycerol and fatty acid. Optimal reaction time at 35 C reaction temperature was found to be three hr.

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Authors and Affiliations

  1. Berol Kemi AB, S-444 Ol Stenungsund, Box 851, Sweden

    K. Holmberg & E. Osterberg

Authors
  1. K. Holmberg
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  2. E. Osterberg
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Holmberg, K., Osterberg, E. Enzymatic preparation of monoglycerides in microemulsion. J Am Oil Chem Soc 65, 1544–1548 (1988). https://doi.org/10.1007/BF02898325

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  • DOI: https://doi.org/10.1007/BF02898325

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