Abstract
Demand for fatty acids is increasing at an annual rate of 17%, due to their increased use in the oleochemical and transport industries. Presently, vegetable oils are the major source of fatty acids, whereas lipids with fatty acids similar to those of some vegetable oils have been reported to be synthesized by oleaginous microorganisms. In the present study, the culturing conditions for the oleaginous yeast Rhodotorula minuta IIP-33 have been optimized. In contrast to the lipid accumulation characteristics of most oleaginous yeasts, a carbon-to-nitrogen ratio of 30 was favorable for maximal accumulation of lipids (48%) with 22.5% conversion of glucose as carbon substrate. The lipids contained fatty acids in the C7–C18 range, the relative composition of which varied with culture temperature.
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References
Borgelt, S.C., T.S. Kolb, and L.G. Schumacher, Biodiesel: World Status, Liq. Fuel Luber. Addit. Biomass, Proc. Altern. Energy Conf., edited by Bruce E. Dale, American Society of Agricultural Engineers, St. Joseph, Michigan, 1994, pp. 67–76.
Harlet, P., Use of Synthetic Esters for Biodegradable Lubricants, Proc. Conf. on Microbiology in the Oil Industry and Lubricants, Sopron, Hungary, September 10–12, 1991, edited by A. Zakar, Hungarian Chemical Society, Budapest, pp. 79–99.
Ratledge, C., and C.A. Boulton, Fats and Oils, in Comprehensive Biotechnology, edited by Murray Moo-Young, Pergamon Press, New York, 1985, pp. 983–1003.
Ratledge, C., Microbial Oils and Fats: An Assessment of Their Commercial Potential, in Prog. Ind. Microbiol., edited by M.J. Bull, Elsevier Scientific Publishers, Oxford, 1982, pp. 119–206.
Evans, C.T., and C. Ratledge, Effect of Nitrogen Source on Lipid Accumulation in Oleaginous Yeasts, J. Gen. Microbiol. 130:1693–1704 (1984).
Suutari, M., K. Liukkonen, and S. Laakso, Temperature Adaptation in Yeasts: The Role of Fatty Acids, Ibid.:1469–1474 (1990).
Folch, J., M. Lees, and G.H.S. Stanley, A Simple Method for the Isolation and Purification of Total Lipids from Animal Tissues, J. Biol. Chem. 226:497–509 (1957).
Morris, D.A., Chemical Analysis of Microbial Cells, in Methods in Microbiology, Vol. 5B, edited by J.R. Norris and D.W. Ribbons, Academic Press, New York, 1948, pp. 210–344.
Official Methods of Analysis, 13th edn., Association of Official Analytical Chemists, Washington, D.C., 1980, pp. 552–553.
Schmitz, A.A., and L.D. Metcalfe, The Rapid Preparation of Fatty Acid Esters for GC Analysis, Anal. Chem. 33:364 (1961).
Jacob, Z., and M.N. Krishnamurthy, Studies on Physico-chemical Characteristics and Fatty Acid Composition of Lipid Produced by a Strain of Rhodotorula glutinis CFR-1, J. Am. Oil Chem. Soc. 67:642–645 (1990).
Suutari, T., P. Priha, and S. Laakso, Temperature Shifts in Regulation of Lipids Accumulated by Lipomyces starkeyi, Ibid.:891–894 (1993).
Johnson, V.W., M. Singh, V.S. Saini, D.K. Adhikari, V.R. Sista, and N.K. Yadav, Utilisation of Molasses for the Production of Fat by an Oleaginous Yeast Rhodotorula glutinis IIP-30, J. Ind. Microbiol. 14:1–4 (1995).
Patel, H., U. Trivedi, and R. Ray, Effect of Carbon, Nitrogen Source and Divalent Cations on Lipids Yield and Fatty Acids Profile of Rhodotorula minuta, in Industrial Biotechnology, edited by V.S. Malik and P. Sridhar, Oxford and IBH Publishing Co. Ltd., New Delhi, 1992, pp. 533–540.
Chen, H.-C., and C.-C. Chang, Production of γ-Linolenic Acid by the Fungus Cunninghamella echinulata CCRC 31840, Biotechnol. Prog. 12:338–341 (1996).
Brenner, R.R., Effect of Unsaturated Acids on Membrane Structure and Enzyme Kinetics, Prog. Lipid Res. 23:69 (1984).
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Saxena, V., Sharma, C.D., Bhagat, S.D. et al. Lipid and fatty acid biosynthesis by Rhodotorula minuta . J Amer Oil Chem Soc 75, 501–505 (1998). https://doi.org/10.1007/s11746-998-0254-x
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DOI: https://doi.org/10.1007/s11746-998-0254-x