Summary
Rhodotorula glutinis is known to produce fat when cultivated under nitrogen-limiting conditions. Economically, molasses is an ideal substrate, however, due to the presence of nitrogen in molasses, the lipid yield obtained is much lower than that obtained from glucose or sucrose. Higher yields were obtained using molasses in a fed batch fermentation supplemented with glucose or sucrose during the lipid accumulation phase. The fatty acids profile of the lipids thus produced, using a very simple and economical medium, was similar to that obtained from glucose and sucrose.
Similar content being viewed by others
References
Almazan, O., M. Klibanky and M.A. Otero. 1981. Microbial fat synthesis byRhodotorula glutinis from black strap molasses in continuous culture. Biotech. Lett. 3: 663–666.
Atamanyuk, D.I. and L.I. Vakar. 1975. Lipidy Gribov-Shtiintsa (A.I. Garkavenko, ed.), pp. 98–102, Kishnov, USSR.
Folch, J., M. Lees and G.H. Sloane-Stanely. 1957. A simple method for the isolation and purification of total lipids from animal tissue. J. Biol. Chem. 226: 497–509.
Fong, J., F.L. Schoffer and P.C. Kirk. 1953. The ultramicro determination of glucose in liver: a comparison of the anthrone and reducing sugar methods. Arch. Biochem. Biophys. 45: 319–326.
Glatz, B.A., M.D. Floetenmeyer and E.G. Hammond. 1985. Fermentation of bananas and other food wastes to produce microbial lipid. J. Food Prot. 48: 574–577.
Granger, L.M., P. Perlot, G. Goma and A. Pareilleux. 1992. Kinetics of growth and fatty acid production ofRhodotorula glutinis. Appl. Microbiol. Biotechnol. 37: 13–17.
Jacob, Z. and M.N. Krishnamurthy. 1990. Studies on physicochemical characteristics and fatty acid composition of lipid produced by a strain ofRhodotorula gracilis CFR-1. J. Am. Oil Chem. Soc. 67: 642–645.
Johnson, V., M. Singh, V.S. Saini, V.R. Sista and N.K. Yadav. 1992. Effect of pH on lipid accumulation by an oleaginous yeast:Rhodotorula glutinis IIP.30. World J. Microbial. Biotechnol. 8(4): 382–384.
Johnson, V., M. Singh, V.S. Saini, D.K. Adhikari, V.R. Sista and N.K. Yadav. 1992. Bioemulsifier production by an oleaginous yeastRhodotorula glutinis IIP.30. Biotech. Lett. 14: 487–490.
Leman, J., W. Bednarski and J. Tomasik. 1990. Influence of cultivation conditions on the composition of oil produced byCandida curvata D. Biol. Wastes 31: 1–15.
Misra, S., A. Ghosh and J. Dutta. 1984. Production and composition of microbial fat fromRhodotorula glutinis. J. Sci. Food Agric. 35: 59–65.
Nayak, N.K., M.G. Hirlekar and N.K. Pandey. 1987. Conditions for fat production by a recombinant strain of yeast. J. Ferment. Technol. 65: 549–553.
Ratledge, C. 1982. Microbial oils and fats: an assessment of their commercial potential. Prog. Ind. Microbiol. 16: 119–206.
Slater, N.K.H. 1988. Economic aspects of lipid biotechnology. In: World Conference for the Fat Oils Industry (Applewhite, T.H., ed.), pp. 238–243, American Oil Chemists Society. Champaign, Ill.
Standard Methods for the Examination of Water and Waste Water. 1989. 17th edn, American Health Association, Washington DC, USA.
Woodbine, M. 1959. Microbial fat: microorganisms as potential fat producers. Prog. Ind. Microbiol. 1: 181–245.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Johnson, V.W., Singh, M., Saini, V.S. et al. Utilization of molasses for the production of fat by an oleaginous yeast,Rhodotorula glutinis IIP-30. Journal of Industrial Microbiology 14, 1–4 (1995). https://doi.org/10.1007/BF01570057
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01570057