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Proteins from Crambe abyssinica oilseed. II. Biochemical and functional properties

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Journal of the American Oil Chemists' Society

Abstract

Little information is available concerning the properties of proteins that constitute a major component of the seed of Crambe abyssinica. Therefore, a method was developed to isolate these proteins. This procedure resulted in two fractions, an isoelectric precipitate (Fraction 1) and a retentate after ultrafiltration (Fraction 2). Biochemical and functional properties of both fractions were studied. Gel permeation chromatography revealed that high-molecular-weight proteins (>669,000 Da) are present only in Fraction 1, whereas Fraction 2 consists of proteins with lower molecular weights (<200,000 Da). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, in the presence of mercaptoethanol, showed that both fractions consist of proteins that range mainly from 40,000 to less than 14,400 Da. Fraction 1 was highly soluble only at acid and alkaline pH values, while the solubility of Fraction 2 remained high (>80%) over the whole pH range tested. Addition of NaCl did not have any profound effect on the solubility of Fraction 2, but it increased significantly that of Fraction 1 in the isoelectric range. Foaming properties of Fraction 1 were better than those of chicken egg white only at pH 9, whereas those of Fraction 2 were superior at almost every pH value studied. Addition of NaCl improved significantly the foaming properties of Fraction 1 at all pH values tested but did not have a profound effect on the foaming properties of Fraction 2. Both fractions had good emulsifying properties only at alkaline pH values.

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Authors and Affiliations

  1. Agrotechnological Research Institute (ATO-DLO), 6700 AA, Wageningen, The Netherlands

    E. Massoura, J. M. Vereijken, P. Kolster & J. T. P. Derksen

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  1. E. Massoura
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  2. J. M. Vereijken
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  3. P. Kolster
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  4. J. T. P. Derksen
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Massoura, E., Vereijken, J.M., Kolster, P. et al. Proteins from Crambe abyssinica oilseed. II. Biochemical and functional properties. J Amer Oil Chem Soc 75, 329–335 (1998). https://doi.org/10.1007/s11746-998-0049-0

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  • DOI: https://doi.org/10.1007/s11746-998-0049-0

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