Abstract
Within the framework of enzymatic biosurfactant synthesis, the development of reaction processes leading to a selective and efficient production is of crucial interest. In this work, we proposed an enzymatic method for producing 1(3)-monooleoyl-rac-glycerol using a commercial immobilized lipase, the Lipozyme®. To avoid the blockage of the enzyme by the presence of insoluble glycerol, the latter was adsorbed onto dried silica gel. The selective monoester production (83%) was obtained using 2-methyl-2-butanol amended n-hexane (90:10, vol/vol) as reaction medium. The observed decrease in the substrate conversion, in a medium containing 2-methyl-2-butanol, was countered by coupling to the reaction an inline selective recovery method for the produced monoester by adsorption onto a silica gel column located at the outflow of the reaction vessel. This process leads to an efficient monooleoyl glycerol production with a high oleic acid conversion (71%) and to the recovery of a 100% pure monooleoyl glycerol.
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Edmundo, C., Valérie, D., Didier, C. et al. Efficient lipase-catalyzed production of tailor-made emulsifiers using solvent engineering coupled to extractive processing. J Amer Oil Chem Soc 75, 309–313 (1998). https://doi.org/10.1007/s11746-998-0046-3
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DOI: https://doi.org/10.1007/s11746-998-0046-3