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Mixed culture solid substrate fermentation for cellulolytic enzyme production

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Summary

Cellulolytic and hemicellulolytic enzymes were produced on extracted sweet sorghum silage by mixed culture solid substrate fermentation with Trichoderma reesei LM-1 (a Peruvian mutant) and Aspergillus niger ATCC 10864. Optimal cellulose and xylanase levels of 4 IU/g dry weight (DW) and 180 IU/g DW, respectively, were achieved in 120 h-fermentation when T. reesei, inoculated at 0 h, was followed by the inoculation of A. niger at 48 h.

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Authors and Affiliations

  1. Department of Microbiology, Colorado State University, 80523, Fort Collins, CO, USA

    Maria R. Castillo, James C. Linden & Robert P. Tengerdy

  2. Laboratorio de Micologia y Biotecnologia, Universidad Nacional Agraria La Molina, Apartado 456, 1, Lima, Peru

    Marcel Gutierrez-Correa

Authors
  1. Maria R. Castillo
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  2. Marcel Gutierrez-Correa
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  3. James C. Linden
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  4. Robert P. Tengerdy
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Castillo, M.R., Gutierrez-Correa, M., Linden, J.C. et al. Mixed culture solid substrate fermentation for cellulolytic enzyme production. Biotechnol Lett 16, 967–972 (1994). https://doi.org/10.1007/BF00128635

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