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Solvent and Extraction Conditions Control the Assayable Phenolic Content and Antioxidant Activities of Seeds of Black Beans, Canola and Millet

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Journal of the American Oil Chemists' Society

Abstract

The effects of extraction solvent and conditions on the total phenolic content (TPC) and antioxidant activity of black beans, canola and foxtail millet were investigated. The antioxidant activity was assayed using 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DRSA) and oxygen radical absorbance capacity (ORAC). Four solvent systems, namely 70 % acetone, 80 % ethanol, 80 % methanol and a mixture of acetone/methanol/water (7:7:6, v/v/v) were used. The extraction methods adopted in this study included refluxing, homogenization, cold extraction and sonication. The TPC as measured using the Folin Ciocalteu’s method were 12.35–28.39, 2.43–16.73, and 1.78–5.06 µmol catechin equivalents/g dry matter (dm) for canola, black beans and foxtail millet, respectively. Aqueous acetone afforded the highest TPC for black beans and canola. Within the same solvent system used, the TPC, DRSA and ORAC obtained from different extraction techniques differed for black beans, canola and foxtail millet. The results demonstrated that the solvent system as well as method influenced the extraction of phenolic compounds and their antioxidant activities, depending on the type of matrix in which phenolics were embedded.

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Acknowledgments

This research was supported by the Natural Science and Engineering Research Council (NSERC) of Canada through a Discovery Grant to FS.

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Authors and Affiliations

  1. Department of Applied Nutrition, Wayamba University of Sri Lanka, Makandura, Gonawila, 60170, Sri Lanka

    Anoma Chandrasekara

  2. Department of Biochemistry, Memorial University of Newfoundland, St. John’s, NL, A1B 3X9, Canada

    Anoma Chandrasekara, Omar Abdel Rasek, Jenny Ann John, Neel Chandrasekara & Fereidoon Shahidi

  3. Department of Agriculture, Sri Lanka School of Agriculture, Kundasale, Sri Lanka

    Neel Chandrasekara

Authors
  1. Anoma Chandrasekara
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  2. Omar Abdel Rasek
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Correspondence to Anoma Chandrasekara.

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Chandrasekara, A., Rasek, O.A., John, J.A. et al. Solvent and Extraction Conditions Control the Assayable Phenolic Content and Antioxidant Activities of Seeds of Black Beans, Canola and Millet. J Am Oil Chem Soc 93, 275–283 (2016). https://doi.org/10.1007/s11746-015-2760-y

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  • DOI: https://doi.org/10.1007/s11746-015-2760-y

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