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Oil extraction from microalga Nannochloropsis sp. with isopropyl alcohol

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Journal of the American Oil Chemists' Society

Abstract

An efficient process for fractioning microalgae oil and non-lipid biomass was developed. Isopropyl alcohol (IPA) was used to extract oil from Nannochloropsis sp. at 80 °C, leaving the majority of non-lipid biomass in the solid fraction. The effectiveness of extraction with or without a dewatering pretreatment (DW) was compared. Effects of dewatering time and solvent ratio, IPA concentration, IPA refluxing time, and sonication pretreatment on the oil and biomass yields were studied. The dewatering conditions with a high water-to-alcohol ratio (W/A = 2:1) and mild mixing (1 min gentle shaking) had 14 % less oil loss in the DW fraction than that with a low water-to-alcohol ratio (W/A = 1:1) and vigorous mixing (30 min and 300 rpm mixing). Sonication resulted in 14–26 % more oil loss in the DW fraction when compared to intact cell treatment. Without dewatering, 85 % of the total oil from intact cells was extracted by a single extraction using 70 % (wt) IPA aqueous solution. The 88 and 95 % IPA treatments extracted similar percentages of oil to that of the 70 % IPA, but used two- and fivefold more solvent. The amount of oil extracted from broken cells increased with increasing IPA concentrations. An effective extraction can be completed in 30 min. On a 100-g (wet matter) scale, the 70 % IPA achieved 92 % oil yield and 93 % non-lipid biomass yield.

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References

  1. US Department of Energy (2010) National algal biofuels technology roadmap. US Department of Energy, Office of Energy Efficiency and Renewable Energy, Biomass Program

  2. Harwood JL, Jones LA (1989) Lipid metabolism in algae. Adv Bot Res 16:1–53

    Article  CAS  Google Scholar 

  3. Wang G, Wang T (2012) Characterization of lipid components in two microalgae for biofuel application. J Am Oil Chem Soc 89:135–143

    Article  CAS  Google Scholar 

  4. Schäfer K (1998) Accelerated solvent extraction of lipids for determining the fatty acid composition of biological material. Anal Chim Acta 358:69–77

    Article  Google Scholar 

  5. Herrero M, Cifuentes A, Ibanez E (2006) Sub- and supercritical fluid extraction of functional ingredients from different natural sources: plants, food-by-products, algae and microalgae. Food Chem 98:136–148

    Article  CAS  Google Scholar 

  6. Ramírez Fajardo A, Cerdán LE, Robles Medina A, Acién Fernández FG, González Moreno PA, Molina Grima E (2007) Lipid extraction from the microalga Phaeodactylum tricornutum. Eur J Lipid Sci Tehnol 109:120–126

    Article  Google Scholar 

  7. Johnson LA, Lusas EW (1983) Comparison of alternative solvents for oils extraction. J Am Oil Chem Soc 60:229–242

    Article  CAS  Google Scholar 

  8. Palacios LE, Wang T (2005) Egg-yolk lipid fractionation and lecithin characterization. J Am Oil Chem Soc 82:571–578

    Article  CAS  Google Scholar 

  9. Wang G, Wang T (2012) Lipid and biomass distribution and recovery from two microalgae by aqueous and alcohol processing. J Am Oil Chem Soc 89:335–345

    Article  CAS  Google Scholar 

  10. Christie WW, Han X (2010) Lipid analysis: isolation, separation, identification and lipidomic analysis, 4th edn. The Oily Press, Bridgewater, England, pp 63–64

    Google Scholar 

  11. Mercer P, Armenta RE (2011) Developments in oil extraction from microalgae. Eur J Lipid Sci Technol 113:539–547

    Article  CAS  Google Scholar 

  12. Kale A (2011) Methods of and systems for isolating nutraceutical products from algae. US patent 8,084, 038

  13. Lee J-Y, Yoo C, Jun S-Y, Ahn C-Y, Oh H-M (2010) Comparison of several methods for effective lipid extraction from microalgae. Biores Technol 101:S75–S77

    Article  CAS  Google Scholar 

  14. Ryckebosch E, Muylaert K, Foubert I (2012) Optimization of an analytical procedure for extraction of lipids from microalgae. J Am Oil Chem Soc 89:189–198

    Article  CAS  Google Scholar 

  15. Cooney M, Young G, Nagle N (2009) Extraction of bio-oils from microalgae. Sep Purif Rev 38:291–325

    Article  CAS  Google Scholar 

  16. Hu W, Wells JH, Shin T-S, Godber JS (1996) Comparison of isopropanol and hexane for extraction of vitamin E and oryzanols from stabilized rice bran. J Am Oil Chem Soc 73:1653–1656

    Article  CAS  Google Scholar 

  17. Molina Grima E, Robles Medina A, Giménez Giménez A, Sánchez Pérez JA, Garcia Camacho F (1994) Comparison between extraction of lipids and fatty acids from microalgal biomass. J Am Oil Chem Soc 71:955–959

    Article  CAS  Google Scholar 

  18. Halim R, Gladman B, Danquah MK, Webley PA (2011) Oil extraction from microalgae for biodiesel production. Biores Technol 102:178–185

    Article  CAS  Google Scholar 

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Acknowledgments

This research was funded by ConocoPhillips.

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Authors and Affiliations

  1. Department of Food Science and Human Nutrition, Center for Crops Utilization Research, Iowa State University, 2312 Food Sciences Building, Ames, IA, USA

    Linxing Yao, Jose A. Gerde & Tong Wang

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  1. Linxing Yao
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  2. Jose A. Gerde
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  3. Tong Wang
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Correspondence to Tong Wang.

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Yao, L., Gerde, J.A. & Wang, T. Oil extraction from microalga Nannochloropsis sp. with isopropyl alcohol. J Am Oil Chem Soc 89, 2279–2287 (2012). https://doi.org/10.1007/s11746-012-2124-9

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  • DOI: https://doi.org/10.1007/s11746-012-2124-9

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