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Generation and remodeling of highly polyunsaturated molecular species of rat hepatocyte phospholipids

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Lipids

Abstract

Freshly isolated rat hepatocytes were incubated for 20 min with [U-14C]glycerol in the presence or absence of unlabeled linoleic (18∶2n-6), arachidonic (20∶4n-6), or docosahexaenoic (22∶6n-3) acid, added as albumin complex in 10% ethanol. Most of the radioactivity (≈95%) recovered in hepatocyte lipids was present in phosphatidylcholine (PC), phosphatidylethanolamine (PF), and triacylglycerol (TAG). The presence of exogenous fatty acids resulted in (i) higher incorporation of [U-14C]glycerol, (ii) higher percentage of label in TAG, and (iii) enhanced formation of PC and PE molecular species bearing the exogenous fatty acid at both the sn-1 and sn-2 positions of glycerol. In each case, these molecular species contained 60 to 70% of the label in that lipid class. Further incubation of the cells for 40 and 80 min in the absence of labeled substrate and exogenous fatty acids resulted in a redistribution of label among PC and PE molecular species due to deacylation-reacylation at the sn-1 position of glycerol.

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Abbreviations

DAG:

diacylglycerols

DNB:

dinitrobenzoate

HPLC:

high-performance liquid chromatography

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

TG:

triacylglycerols

TLC:

thin-layer chromatography. Also, 18∶2, 20∶3, 20∶4, 22∶5, and 22∶6 denote 18∶2n-6, 20∶3n-6, 20∶4n-6, 22∶5n-3, and 22∶6n-3, respectively

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Authors and Affiliations

  1. The Hormel Institute, University of Minnesota, 801 16th Ave, NE, 55912, Austin, MN

    Patricia C. Schmid, Ilona Spimrova & Harald H. O. Schmid

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  1. Patricia C. Schmid
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  2. Ilona Spimrova
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  3. Harald H. O. Schmid
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Schmid, P.C., Spimrova, I. & Schmid, H.H.O. Generation and remodeling of highly polyunsaturated molecular species of rat hepatocyte phospholipids. Lipids 32, 1181–1187 (1997). https://doi.org/10.1007/s11745-997-0152-7

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  • DOI: https://doi.org/10.1007/s11745-997-0152-7

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