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Lipid composition of cultured endothelial cells in relation to their growth

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Lipids

Abstract

Human endothelial cells in culture were examined in different growth conditions. The human endothelial cell line, EA.hy 926 cell line, was used and cells were studied either in exponential growth phase, at confluence, or growth-arrested by serum deprivation. Phospholipids were separated and analyzed by high-performance thin-layer chromatography, and their fatty acids were quantified by gas-liquid chromatography. No significant differences in the phospholipid distributions were found between exponentially growing and confluent endothelial cells in which phosphatidylcholine (PC) represented the major phospholipid. In comparison, serum-deprived cells exhibited higher proportions of sphingomyelin and lower content of PC. We also found that among the total lipids, cholesterol level for dividing endothelial cells was lower than for cells growth-arrested either by serum deprivation or by contact inhibition at confluence. The global fatty acid distribution was not affected by the growth conditions. Thus, oleate (18∶1n−9 and 18∶1n-7), palmitate (C16∶0), and stearate (C18∶0) were the main components of endothelial cell membranes. However, the fatty acid distributions obtained from each phospholipid species differed with the growth status. Altogether, the data indicated that subtle modulations of endothelial cell metabolism appear upon cell growth. The resulting membrane-dependent cellular functions such as cholesterol transport and receptor activities can be expected to be relevant for lipid trafficking within the vessel wall in vitro and in vivo.

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Abbreviations

DMEM:

Dulbecco’s minimal essential medium

EDTA:

ethylenediaminetetraacetic acid

EA.hy 926:

human endothelial cell line

FCS:

fetal calf serum

GLC:

gas-liquid chromatography

HAT:

hypoxanthine-aminopterin-thymidine

HDL:

high density lipoprotein

HPLC:

high-performance liquid chromatography

HPTLC:

high-performance thin layer chromatography

LDL:

low density lipoprotein

NL:

neural lipids

PBS:

phosphate buffer saline

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PI:

phosphatidylinositol

PS:

phosphatidylserine

SM:

sphingomyelin

SMC:

smooth muscle cells

TLC:

thin-layer chromatography

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Authors and Affiliations

  1. Biochimie Molécules Marines, IFREMER, DRV/VP, Nantes, France

    Jean-Paul Gouygou

  2. Institut Galilée, Université Paris XIII, LRM, CNRS URA 502, Av. J.B. Clément, 93 430, Villetaneuse, France

    Maud Cansell, Jacqueline Jozefonvicz & Didier Letourneur

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  1. Maud Cansell
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  2. Jean-Paul Gouygou
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  3. Jacqueline Jozefonvicz
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  4. Didier Letourneur
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Cansell, M., Gouygou, JP., Jozefonvicz, J. et al. Lipid composition of cultured endothelial cells in relation to their growth. Lipids 32, 39–44 (1997). https://doi.org/10.1007/s11745-997-0006-3

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  • DOI: https://doi.org/10.1007/s11745-997-0006-3

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