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Gut Microbial Metabolites of Polyunsaturated Fatty Acids Correlate with Specific Fecal Bacteria and Serum Markers of Metabolic Syndrome in Obese Women

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Lipids

Abstract

The aim of this human study was to assess the influence of prebiotic-induced gut microbiota modulation on PUFA-derived bacterial metabolites production. Therefore, we analyzed the circulating fatty acid profile including CLA/CLnA in obese women treated during 3 months with inulin-type fructan prebiotics. In these patients, we had already determined gut microbiota composition by phylogenetic microarray and qPCR analysis of 16S rDNA. Some PUFA-derived bacterial metabolites were detected in the serum of obese patients. Despite the prebiotic-induced modulation of gut microbiota, including changes in CLA/CLnA-producing bacteria, the treatment did not impact significantly on the circulating level of these metabolites. However, some PUFA-derived bacterial metabolites were positively correlated with specific fecal bacteria (Bifidobacterium spp., Eubacterium ventriosum and Lactobacillus spp.) and inversely correlated with serum cholesterol (total, LDL, HDL). These correlations suggest a potential beneficial effect of some of these metabolites but this remains to be confirmed by further investigation.

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Fig. 1
Fig. 2

Abbreviations

CLA:

Conjugated linoleic acid

CLnA:

Conjugated linolenic acid

FA:

Fatty acid

FAME:

Fatty acid methyl ester

HDL:

High density lipoprotein

HITChip:

Human Intestinal Tract Chip

ITF:

Inulin-type fructans

LDL:

Low density lipoprotein

PPAR:

Peroxisome proliferator-activated receptor

TAG:

Triacylglycerol

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Acknowledgments

We would like to thank L. De Vuyst who helped us to quantify Roseburia spp. C.D. benefits from a Danone Institute grant. P.D.C. is a Research Associate from the FRS-FNRS (Fonds de la Recherche Scientifique, Belgium). PDC and NMD are recipients of FSR and FRSM subsidies (Fonds spéciaux de recherches, UCL, Belgium; Fonds de la recherche scientifique médicale, Belgium). PDC is a recipient of ERC Starting grant. This project was supported by a FNRS grant (No. 1.5.095.09F).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Metabolism and Nutrition Research Group, Louvain Drug Research Institute, Université catholique de Louvain, Avenue E. Mounier 73, PO box B1.73.11, 1200, Brussels, Belgium

    Céline Druart, Evelyne M. Dewulf, Patrice D. Cani, Audrey M. Neyrinck & Nathalie M. Delzenne

  2. WELBIO, Walloon Excellence in Life Sciences and Biotechnology, Université catholique de Louvain, Brussels, Belgium

    Patrice D. Cani

  3. Pole of Endocrinology, Diabetology and Nutrition, IREC, Université catholique de Louvain, Brussels, Belgium

    Jean-Paul Thissen

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  1. Céline Druart
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  2. Evelyne M. Dewulf
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  3. Patrice D. Cani
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  4. Audrey M. Neyrinck
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  5. Jean-Paul Thissen
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  6. Nathalie M. Delzenne
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Correspondence to Nathalie M. Delzenne.

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Druart, C., Dewulf, E.M., Cani, P.D. et al. Gut Microbial Metabolites of Polyunsaturated Fatty Acids Correlate with Specific Fecal Bacteria and Serum Markers of Metabolic Syndrome in Obese Women. Lipids 49, 397–402 (2014). https://doi.org/10.1007/s11745-014-3881-z

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  • DOI: https://doi.org/10.1007/s11745-014-3881-z

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