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In Vitro TNF-α- and Noradrenaline-Stimulated Lipolysis is Impaired in Adipocytes from Growing Rats Fed a Low-Protein, High-Carbohydrate Diet

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Lipids

Abstract

The aim of this study was to investigate tumor necrosis factor alpha (TNF-α)- and noradrenaline (NE)-stimulated lipolysis in retroperitoneal (RWAT) and epididymal (EAT) white adipose tissue as a means of understanding how low-protein, high-carbohydrate (LPHC) diet-fed rats maintain their lipid storage in a catabolic environment (marked by increases in serum TNF-α and corticosterone and sympathetic flux to RWAT and EAT), as previously observed. Adipocytes or tissues from the RWAT and EAT of rats fed an LPHC diet and rats fed a control (C) diet for 15 days were used in the experiments. The adipocytes from both tissues of the LPHC rats exhibited lower TNF-α- stimulated lipolysis compared to adipocytes from the C rats. The intracellular lipolytic agents IBMX, DBcAMPc and FSK increased lipolysis in both tissues from rats fed the C and LPHC diets compared to basal lipolysis; however, the effect was approximately 2.5-fold lower in adipocytes from LPHC rats. The LPHC diet induced a marked reduction in the β3 and α2-AR, adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) content in RWAT and EAT. The LPHC diet did not affect TNF-α receptor 1 content but did induce a reduction in ERK p44/42 in both tissues. The present work indicates that RWAT and EAT from LPHC rats have an impairment in the lipolysis signaling pathway activated by NE and TNF-α, and this impairment explains the reduced response to these lipolytic stimuli, which may be fundamental to the maintenance of lipid storage in LPHC rats.

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Abbreviations

TNF-α:

Tumor necrosis factor alpha

NE:

Noradrenaline

RWAT:

Retroperitoneal white adipose tissue

EAT:

Epididymal white adipose tissue

LPHC:

Low-protein, high-carbohydrate diet

ATGL:

Adipose triglyceride lipase

HSL:

Hormone-sensitive lipase

TAG:

Triacylglycerol(s)

PLIN:

Perilipin

PAT:

Perilipin, adipophilin/adipocyte differentiation-related protein and tail-interacting protein of 47 kDa

ADRP:

Adipocyte differentiation-related protein

TIP47:

Tail-interacting protein of 47 kA

PKA:

Protein kinase A

FA:

Fatty acid(s)

DAG:

Diacylglycerol

CGI-58:

Comparative gene identification-58

β3-AR:

β3-Adrenergic receptor

Gs:

Stimulated G protein

cAMP:

Cyclic adenosine monophosphate

α2-AR:

α2-Adrenergic receptor

TNFR-1:

Isoform 1 TNF receptor

MAPK:

Mitogen-activated protein kinase

ERK:

Extracellular signal-regulated kinase

JNK:

c-Jun NH2-terminal kinase

SAPK:

Stress-activated protein kinase

DBcAMP:

Dibutyryl cAMP

FSK:

Forskolin

IBMX:

Isobutylmethylxanthine

PMSF:

Phenylmethylsulfonyl fluoride

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Acknowledgments

We thank Air Francisco Costa and Celso Roberto Afonso for their technical assistance. This work was supported by grants from Fundação de Amparo à Pesquisa do Estado de Mato Grosso (FAPEMAT), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

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Authors and Affiliations

  1. Department of Chemistry, Federal University of Mato Grosso, Cuiabá, MT, Brazil

    Daniel D. S. Feres, Maísa P. dos Santos, Samyra L. Buzelle, Mayara P. Pereira, Suélem A. de França, Cláudia M. B. Andrade, Mendalli Froelich, Fhelipe J. S. de Almeida & Nair H. Kawashita

  2. Department of Physiology, University of São Paulo, Ribeirão Preto, SP, Brazil

    Maria A. R. Garófalo

  3. Institute of Environment, Nature and Science Life, Federal University of Latin American Integration, Foz do Iguaçu, PR, Brazil

    Danúbia Frasson

  4. Laboratory of Physiology and Pharmacology, Federal University of São João Del Rei, Divinópolis, MG, Brazil

    Valéria E. Chaves

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  1. Daniel D. S. Feres
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  2. Maísa P. dos Santos
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  3. Samyra L. Buzelle
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  12. Nair H. Kawashita
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Correspondence to Nair H. Kawashita.

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Feres, D.D.S., dos Santos, M.P., Buzelle, S.L. et al. In Vitro TNF-α- and Noradrenaline-Stimulated Lipolysis is Impaired in Adipocytes from Growing Rats Fed a Low-Protein, High-Carbohydrate Diet. Lipids 48, 779–786 (2013). https://doi.org/10.1007/s11745-013-3809-z

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