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Lipid Lowering and Antioxidant Effect of Miglitol in Triton Treated Hyperlipidemic and High Fat Diet Induced Obese Rats

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Lipids

Abstract

Miglitol, an anti-diabetic drug, has been shown to reduce plasma lipids and inhibit free radical generation. The anti-hyperlipidemic and antioxidant effects of miglitol were studied in triton-induced hyperlipidemic rats and high fat diet-fed obese rats. Plasma cholesterol and triglycerides levels were significantly lowered by miglitol at 100 mg/kg body weight doses. Miglitol inhibited generation of superoxide anion and hydroxyl free radicals by 14 and 31 % in enzymatic systems and 19 and 25 % in non-enzymatic systems, respectively. The in-vitro effect of the drug on adipogenesis using 3T3-L1 preadipocytes at 2-, 5- and 10-μM concentrations showed significant inhibition of adipogenesis (34.2 %) at 10-μM concentration. High fat diet-fed rat model was used to investigate anti-hyperlipidemic, anti-obesity and antioxidant effect of miglitol. Miglitol increased the activities of lecithin-cholesterol-acyltransferase (19 %), post heparin lipolytic activity (26 %), lipoprotein lipase (26 %) and triglyceride lipase (31 %) which result in a decrease in plasma lipid levels. The antioxidant enzymes viz., catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase and thioredoxin reductase were increased by the drug in the treated animals. The antihyperlipidemic and antioxidant effect of miglitol can be correlated to its effect on different enzymes and it can be used for inhibiting the development of cardiovascular diseases.

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Abbreviations

HFD:

High fat diet

LCAT:

Lecithin-cholesterol acyltransferase

PHLA:

Post heparin lipolytic activity

LPL:

Lipoprotein lipase

TGL:

Triglyceride lipase

TC:

Total cholesterol

TG:

Triglycerides

PL:

Phospholipids

HDL:

High density lipoprotein

LDL:

Low density lipoprotein

VLDL:

Very low density lipoprotein

Mig:

Miglitol

CVD:

Cardiovascular diseases

HMG:

3-Hydroxy-3-methyl-glutaryl

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Acknowledgments

All the work was supported by the Council of Scientific and Industrial Research, New Delhi (India). CDRI Communication No.: 8376.

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There is no conflict of interest among authors.

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Authors and Affiliations

  1. Biochemistry Division, CSIR-Central Drug Research Institute, 1 M.G. Marg, Lucknow, 226001, India

    Atul Shrivastava, Upma Chaturvedi, Shiv Vardan Singh, Jitendra Kumar Saxena & Gitika Bhatia

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  1. Atul Shrivastava
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Correspondence to Gitika Bhatia.

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Shrivastava, A., Chaturvedi, U., Singh, S.V. et al. Lipid Lowering and Antioxidant Effect of Miglitol in Triton Treated Hyperlipidemic and High Fat Diet Induced Obese Rats. Lipids 48, 597–607 (2013). https://doi.org/10.1007/s11745-012-3753-3

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