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Increased Lipid Peroxidation in LDL from Type-2 Diabetic Patients

  • Original Article
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Lipids

Abstract

Increased oxidative stress is associated with type-2 diabetes and related cardiovascular diseases, but oxidative modification of LDL has been partially characterized. Our aim was to compare the lipid and fatty acid composition as well as the redox status of LDL from diabetic patients and healthy subjects. First, to ensure that isolation of LDL by sequential ultracentrifugation did not result in lipid modifications, lipid composition and peroxide content were determined in LDL isolated either by ultracentrifugation or fast-protein liquid chromatography. Both methods resulted in similar concentrations of lipids, fatty acids, hydroxy-octadecadienoic acid (HODE) and malondialdehyde (MDA). Then, LDLs were isolated by ultracentrifugation from eight type-2 diabetic patients and eight control subjects. Compared to control LDL, diabetic LDL contained decreased cholesteryl esters and increased triglyceride concentrations. Ethanolamine plasmalogens decreased by 49%. Proportions of linoleic acid decreased in all lipid classes, while proportions of arachidonic acid increased in cholesteryl esters. Total HODE concentrations increased by 56%, 12- and 15-hydroxy-eicosatetraenoic acid by 161 and 86%, respectively, and MDA levels increased by twofold. α-Tocopherol concentrations, expressed relative to triglycerides, were lower in LDL from patients compared to controls, while γ-tocopherol did not differ. Overall, LDL from type-2 diabetic patients displayed increased oxidative stress. Determination of hydroxylated fatty acids and ethanolamine plasmalogen depletion could be especially relevant in diabetes.

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Abbreviations

BHT:

Butylhydroxytoluene

CE:

Cholesteryl ester

DMA:

Dimethylacetal

ELSD:

Evaporative light-scattering detector

Etn-PL:

Ethanolamine phospholipids

FPLC:

Fast-protein liquid chromatography

GPE:

Glycerophosphoethanolamine

HETE:

Hydroxy-eicosatetraenoic acid

HODE:

Hydroxy-octadecadienoic acid

MDA:

Malondialdehyde

MUFA:

Monounsaturated fatty acids

PC:

Phosphatidylcholine

PL:

Phospholipid

PUFA:

Polyunsaturated fatty acids

SFA:

Saturated fatty acids

SM:

Sphingomyelin

TBA:

Thiobarbituric acid

TBARS:

Thiobarbituric acid reactive species

TG:

Triacylglycerols

UC:

Ultracentrifugation

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Acknowledgments

We thank the subject participants for their contribution. We also thank the nursing staff at the Hospices Civils de Lyon for their help with blood sample collection and some analyses. We acknowledge the French Ministry of Education and Research for RC’s grant. CC is supported by CNRS.

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Authors and Affiliations

  1. Université de Lyon, 69622, Lyon, France

    Romain Colas, Valérie Pruneta-Deloche, Michel Guichardant, Céline Luquain-Costaz, Hubert Vidal, Philippe Moulin, Michel Lagarde & Catherine Calzada

  2. INSA-Lyon, RMND, 69621, Villeurbanne, France

    Romain Colas, Valérie Pruneta-Deloche, Michel Guichardant, Céline Luquain-Costaz, Hubert Vidal, Philippe Moulin, Michel Lagarde & Catherine Calzada

  3. INSERM U870, INSA-Lyon, Régulations métaboliques, Nutrition et Diabètes, Bât Louis Pasteur, INSA-Lyon, IMBL, 20 avenue Albert Einstein, 69621, Villeurbanne, France

    Romain Colas, Valérie Pruneta-Deloche, Michel Guichardant, Céline Luquain-Costaz, Hubert Vidal, Philippe Moulin, Michel Lagarde & Catherine Calzada

  4. INRA U1235, 69921, Oullins, France

    Romain Colas, Valérie Pruneta-Deloche, Michel Guichardant, Céline Luquain-Costaz, Hubert Vidal, Philippe Moulin, Michel Lagarde & Catherine Calzada

  5. Hospices Civils de Lyon, 69003, Lyon, France

    Romain Colas, Valérie Pruneta-Deloche, Michel Guichardant, Céline Luquain-Costaz, Christine Cugnet-Anceau, Myriam Moret, Hubert Vidal, Philippe Moulin, Michel Lagarde & Catherine Calzada

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  1. Romain Colas
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  2. Valérie Pruneta-Deloche
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  3. Michel Guichardant
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  4. Céline Luquain-Costaz
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  5. Christine Cugnet-Anceau
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  6. Myriam Moret
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  7. Hubert Vidal
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  8. Philippe Moulin
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  9. Michel Lagarde
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  10. Catherine Calzada
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Corresponding author

Correspondence to Catherine Calzada.

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Colas, R., Pruneta-Deloche, V., Guichardant, M. et al. Increased Lipid Peroxidation in LDL from Type-2 Diabetic Patients. Lipids 45, 723–731 (2010). https://doi.org/10.1007/s11745-010-3453-9

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  • DOI: https://doi.org/10.1007/s11745-010-3453-9

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