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Conjugated linoleic acid and chromium lower body weight and visceral fat mass in high-fat-diet-fed mice

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Lipids

Abstract

More than half of the U.S. population has a body mass index of 25 kg/m2 or more, which classifies them as overweight or obese. Obesity is often associated with comorbidities such as diabetes, cardiovascular diseases, and cancer. CLA and chromium have emerged as major dietary supplements that reduce body weight and fat mass, and increase basal metabolic rate in animal models. However, studies show that CLA induces insulin resistance in mice and in humans, whereas Cr improves insulin sensitivity. Hence, we designed the present study to examine the combined effect of CLA and Cr on body composition and insulin sensitivity in a Balb/c mice (n=10/group) model of high-fat-diet-induced obesity. CLA alone lowered body weight, total body fat mass, and visceral fat mass, the last of which decreased further with the combination of CLA and Cr. This effect was accompanied by decreased serum leptin levels in CLA-fed and CLA+Cr-fed mice, and by higher energy expenditure (EE) and oxygen consumption (OC) in CLA+Cr-fed mice. Serum levels of glucose, insulin, the pro-inflammatory cytokines, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6), as well as insulin resistance index (IRI), decreased with CLA, whereas CLA and Cr in combination had significant effects on insulin and IL-6 concentrations and IRI. In summary, CLA+Cr decreased body weight and fat mass in high-fat-diet-fed mice, which may be associated with decreased leptin levels and higher EE and OC.

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Abbreviations

AKP:

alkaline phosphatase

BFM:

total body fat mass

BLM:

total body lean mass

CO:

corn oil

c9t11:

cis-9, trans-11

DEXA:

dual energy x-ray absorptiometry

EE:

energy expenditure

IL:

interleukin

IR:

insulin resistance

IRI:

insulin resistance index

IRK:

insulin receptor kinase

IRTP:

insulin receptor tyrosine phosphatase

IS:

insulin sensitivity

LA:

linoleic acid

NF-κB:

nuclear factor-kappaB

OC:

oxygen consumption

PPAR-γ:

peroxisome proliferator-activated receptor-γ

SFO:

safflower oil

t10C12:

trans-10, cis-12

TNF:

tumor necrosis factor

UCP2:

uncoupling protein 2

VFM:

visceral fat mass

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Authors and Affiliations

  1. Department of Medicine, Division of Clinical Immunology, University of Texas Health Science Center, 78229, San Antonio, Texas

    Arunabh Bhattacharya, M. Mizanur Rahman & Gabriel Fernandes

  2. Department of Physiology, University of Texas Health Science Center, 78229, San Antonio, Texas

    Roger McCarter

  3. Loders Croklaan Lipid Nutrition, 55410, Minneapolis, Minnesota

    Marianne O'Shea

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  1. Arunabh Bhattacharya
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  2. M. Mizanur Rahman
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  3. Roger McCarter
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  4. Marianne O'Shea
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  5. Gabriel Fernandes
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Correspondence to Gabriel Fernandes.

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Bhattacharya, A., Rahman, M.M., McCarter, R. et al. Conjugated linoleic acid and chromium lower body weight and visceral fat mass in high-fat-diet-fed mice. Lipids 41, 437–444 (2006). https://doi.org/10.1007/s11745-006-5117-3

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