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Regiospecificity profiles of storage and membrane lipids from the gill and muscle tissue of atlantic salmon (Salmo salar L.) grown at elevated temperature

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Lipids

Abstract

Regiospecific and traditional analysis, of both storage and membrane lipids, was performed on gill, white muscle, and red muscle samples taken from Atlantic salmon (Salmo salar) to gauge the effect of elevated water temperature. The fish, fed a commercial diet, were held at an elevated water temperature of 19°C. Total n-3 PUFA, total PUFA, and n-3/n-6 and unsaturated/saturated fatty acid (UFA/SFA) ratios in the FA profile of the total lipid extract in the white muscle were fairly low compared with fish grown at 15°C. Adaptation of structural and storage lipids at elevated temperatures was shown by a significant (P<0.01) reduction in PUFA especially in the percentage of EPA (6–8%). Further adaptation was indicated by the percentages of SFA, which were significantly (P<0.05) higher in gill (56%) and white muscle (58%) polar lipid fractions and coincided with lower percentages of n-3, n-6, and total PUFA. The regiospecific profiles indicated a high affinity of DHA to the sn-2 position in both the TAG (61–68%) and polar lipid (35–60%) fractions. The combination of detailed regiospecific and lipid analyses demonstrated adaptation of cell membrane structure in Atlantic salmon grown at an elevated water temperature.

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Abbreviations

CDCl3 :

detiterated chloroform

DPA:

docosapentaenoic acid

ESI-RP-LCMS:

electrospray ionization reversed-phase liquid chromatography-MS

LC:

long-chain

MUFA:

monounsaturated FA

PL:

polar lipid

PLFA:

polar lipid FA

SFA:

saturated FA

TLE:

total lipid extract

tr:

trace amounts

UPL:

undetermined PL

UFA:

unsaturated FA

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Authors and Affiliations

  1. School of Aquaculture, Tasmanian Aquaculture and Fisheries Institute, University of Tasmania (UTAS), 7250, Launceston, Tasmania, Australia

    Matthew R. Miller, Julia Barnes & Chris G. Carter

  2. Food Futures Flagship, Marine and Atmospheric Research, CSIRO, 7001, Hobart, Tasmania, Australia

    Matthew R. Miller & Peter D Nichols

  3. Central Science Laboratory, UTAS, 7001, Hobart, Tasmania, Australia

    Noel W. Davies & Evan J. Peacock

Authors
  1. Matthew R. Miller
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  2. Peter D Nichols
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  3. Julia Barnes
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  4. Noel W. Davies
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  6. Chris G. Carter
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Correspondence to Matthew R. Miller.

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Miller, M.R., Nichols, P.D., Barnes, J. et al. Regiospecificity profiles of storage and membrane lipids from the gill and muscle tissue of atlantic salmon (Salmo salar L.) grown at elevated temperature. Lipids 41, 865–876 (2006). https://doi.org/10.1007/s11745-006-5042-5

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  • DOI: https://doi.org/10.1007/s11745-006-5042-5

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