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Lymphatic Absorption and Deposition of Various Plant Sterols in Stroke-Prone Spontaneously Hypertensive Rats, a Strain Having a Mutation in ATP binding cassette transporter G5

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Lipids

Abstract

ATP binding cassette transporter G5 (ABCG5) and ATP binding cassette transporter G8 (ABCG8) have been suggested to transport absorbed plant sterols and cholesterol from enterocytes to the intestinal lumen and from hepatocytes to bile. It has been thought that mutations of ABCG5 or ABCG8 cause the deposition of plant sterols in the body. In the present study, lymphatic absorption of various plant sterols and their deposition in various tissues was investigated in stroke-prone spontaneously hypertensive rats (SHRSP), having a mutation in Abcg5 and depositing plant sterols in the body. The order of lymphatic 24-h recovery of plant sterols was as follows: campesterol > sitosterol > brassicasterol > stigmasterol = sitostanol. When SHRSP were fed a diet containing one of the plant sterols, the depositions of campesterol and sitosterol were comparatively higher than those of brassicasterol, stigmasterol and sitostanol. Highly positive correlations were obtained between lymphatic recovery of plant sterols and their levels in plasma, liver, adipose tissue and heart. The tendency of differential absorption of plant sterols to the lymph in SHRSP was similar to that in normal Wistar rats previously reported by us (Hamada et al. Lipids 41:551–556, 2006). These observations suggest that differential absorption of various plant sterols is kept in SHRSP in spite of a mutation in Abcg5.

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Abbreviations

ABCG5:

ATP binding cassette transporter G5

ABCG8:

ATP binding cassette transporter G8

ACAT:

Acylcoenzyme A:cholesterol acyltransferase

NPC1L1:

Niemann-Pick C1 like 1 protein

SHRSP:

Stroke-prone spontaneously hypertensive rats

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Authors and Affiliations

  1. Laboratory of Nutrition Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, 812-8581, Japan

    Tadateru Hamada, Nami Egashira, Hideaki Nakagiri & Katsumi Imaizumi

  2. Cooperative Research Center, University of Miyazaki, Miyazaki, 889-2192, Japan

    Shoko Nishizono

  3. Department of Environmental and Symbiotic Sciences, Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, Kumamoto, 862-8502, Japan

    Hiroko Tomoyori

  4. Laboratory of Food and Biomolecular Science, Department of Food Function and Health, Division of Bioscience and Biotechnology for Future Bioindustries, Graduate School of Agricultural Science, Tohoku University, Sendai, 981-8555, Japan

    Ikuo Ikeda

Authors
  1. Tadateru Hamada
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  2. Nami Egashira
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  3. Shoko Nishizono
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  4. Hiroko Tomoyori
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  5. Hideaki Nakagiri
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  6. Katsumi Imaizumi
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  7. Ikuo Ikeda
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Correspondence to Ikuo Ikeda.

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Hamada, T., Egashira, N., Nishizono, S. et al. Lymphatic Absorption and Deposition of Various Plant Sterols in Stroke-Prone Spontaneously Hypertensive Rats, a Strain Having a Mutation in ATP binding cassette transporter G5. Lipids 42, 241–248 (2007). https://doi.org/10.1007/s11745-006-3015-3

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  • DOI: https://doi.org/10.1007/s11745-006-3015-3

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