Abstract
Within the Danish population, milk DHA (22∶6n−3) levels vary by more than a factor of 10. This paper deals with fluctuations in the milk content of 22∶6n−3 and other long-chain PUFA (LCPUFA) and the acute effects of fish meals and fish oil supplements on milk levels of LCPUFA. Twelve fish-eating mothers with 4-mon-old infants provided one blood and one adipose tissue sample, and seven consecutive morning hindmilk samples with dietary records from the previous days. Another 12 lactating women were given fish oil (2–8 g) for breakfast and delivered 6–12 milk samples during the following 24 h. The mean milk 22∶6n−3 content of the fish-eating mothers was 0.57±0.28 FA% (=percentage of total area of FAME peaks in GLC) and the day-to-day variation (SD/mean) within the individual was 35±17%. Mean milk 22∶6n−3 content on mornings with no fish the day before was 0.42±0.15 FA%; this was increased by 82±17% (n=9, P=0.05) if the mother had eaten fatty fish. Fish oil resulted in a twofold increase in milk 22∶6n−3 levels, which peaked after 10 h and lasted for 24 h. The EPA content of milk was also increased by fish meals and fish oil supplements, but these had no effect on the level of arachidonic acid. The study showed that diurnal and day-to-day fluctuations in levels of milk n−3 LCPUFA are large, which makes it difficult to assess the 22∶6n−3 intake of breast-fed infants from a single milk sample. In studies of the functional outcome of dietary 22∶6n−3 in breast-fed infants it is suggested also to use a measure of maternal 22∶6n−3 status.
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Abbreviations
- 22∶6n−3 response:
-
area under the curve of milk 22∶6n−3 vs. time (AUC, calculated by the trapezium rule), or the difference between peak and baseline levels of 22∶6n−3 in milk
- AUC:
-
area under the curve of milk FA level vs. time after test meal
- BMI:
-
body mass index (kg/m2)
- FA%:
-
percentage of the total area of FAME peaks in the chromatogram
- LCPUFA:
-
long-chain PUFA
- RBC:
-
erythrocytes
References
Hamosh, M., and Salem, N. (1998) Long-Chain Polyunsaturated Fatty Acids, Biol. Neonate 74, 106–120.
Anderson, J.W., Johnstone, B.M., and Remley, D.T. (1999) Breast-Feeding and Cognitive Development: A Meta-Analysis, Am. J. Clin. Nutr. 70, 525–535.
Gibson, R.A., and Makrides, M. (1998) The Role of Long-Chain Polyunsaturated Fatty Acids (LCPUFA) in Neonatal Nutrition, Acta Paediatr. 87, 1017–1022.
SanGiovanni, J.P., Berkey, C.S., Dwyer, J.T., and Colditz, G.A. (2000) Dietary Essential Fatty Acids, Long-Chain Polyunsaturated Fatty Acids, and Visual Resolution Acuity in Healthy Fullterm Infants: A Systematic Review, Early Hum. Dev. 57, 165–188.
Lauritzen, L., Hansen, H.S., Jørgensen, M.H., and Michaelsen, K.F. (2001) The Essentiality of Long-Chain n−3 Fatty Acids in Relation to Development and Function of the Brain and Retina, Prog. Lipid Res. 40, 1–94.
Jørgensen, M.H., Hernell, O., Hughes, E., and Michaelsen, K.F. (2001) Is There a Relation Between Docosahexaenoic Acid Concentration in Mother’s Milk and Visual Development in Term Infants? J. Pediatr. Gastroenterol. Nutr. 32, 293–296.
Innis, S.M., Gilley, J., Werker, J., MacKinnon, M., and Auestad, N. (2000) Long-Chain Polyunsaturated Fatty Acids, Growth and Neurodevelopment Among Breast-Fed Infants Followed Prospectively to 1 Year of Age, FASEB J. 14, A725 (Abstract).
Scott, D.T., Janowsky, J.S., Carroll, R.E., Taylor, J.A., Auestad, N., and Montalto, M.B. (1998) Formula Supplementation with Long-Chain Polyunsaturated Fatty Acids: Are There Developmental Benefits? Pediatrics 102, E591-E593.
Gibson, R.A., Neumann, M.A., and Makrides, M. (1997) Effect of Increasing Breast-Milk Docosahexaenoic Acid on Plasma and Erythrocyte Phospholipid Fatty Acids and Neural Indices of Exclusively Breast-Fed Infants, Eur. J. Clin. Nutr. 51, 578–584.
Koletzko, B., Thiel, I., and Abiodun, P.O. (1992) The Fatty Acid Composition of Human Milk in Europe and Africa, J. Pediatr. 120, S62-S70.
Jensen, R.G. (1999) Lipids in Human Milk, Lipids 34, 1243–1271.
Innis, S.M., and Kuhnlein, H.V. (1988) Long-Chain n−3 Fatty Acids in Breast-Milk of Inuit Women Consuming Traditional Foods, Early Hum. Dev. 18, 185–189.
Chulei, R., Xiaofang, L., Hongsheng, M., Xiulan, M., Guizheng, L., Gianhong, D., DeFrancesco, C.A., and Connor, W.E. (1995) Milk Composition in Women from Five Different Regions of China: The Great Diversity of Milk Fatty Acids, J. Nutr. 125, 2993–2998.
Harris, W.S., Connor, W.E., and Lindsey, S. (1984) Will Dietary Omega-3 Fatty Acids Change the Composition of Human Milk? Am. J. Clin. Nutr. 40, 780–785.
Henderson, R.A., Jensen, R.G., Lammi-Keefe, C.J., Ferris, A.M., and Dardick, K.R. (1992) Effect of Fish Oil on the Fatty Acid Composition of Human Milk and Maternal and Infant Erythrocytes, Lipids 27, 863–869.
Jensen, C.L., Prager, T.C., Zou, Y.L., Fraley, J.K., Maude, M., Anderson, R.E., and Heird, W.C. (1999) Effects of Maternal Docosahexaenoic Acid Supplementation on Visual Function and Growth of Breast-Fed Term Infants, Lipids 34, S225.
Jensen, C.L., Maude, M., Anderson, R.E., and Heird, W.C. (2000) Effect of Docosahexaenoic Acid Supplementation of Lactating Women on the Fatty Acid Composition of Breast-Milk Lipids and Maternal and Infant Plasma Phospholipids, Am. J. Clin. Nutr. 71, 292S-299S.
Makrides, M., Neumann, M.A., and Gibson, R.A. (1996) Effect of Maternal Docosahexaenoic Acid (DHA) Supplementation on Breast-Milk Composition, Eur. J. Clin. Nutr. 50, 352–357.
Helland, I.B., Saarem, K., Saugstad, O.D., and Drevon, C.A. (1998) Fatty Acid Composition in Maternal Milk and Plasma During Supplementation with Cod Liver Oil, Eur. J. Clin. Nutr. 52, 839–845.
Cherian, G., and Sim, J.S. (1996) Changes in the Breast-Milk Fatty Acids and Plasma Lipids of Nursing Mothers Following Consumption of n−3 Polyunsaturated Fatty Acid Enriched Eggs, Nutrition 12, 8–12.
Fidler, N., Sauerwald, T., Pohl, A., Demmelmair, H., and Koletzko, B. (2000) Docosahexaenoic Acid Transfer into Human Milk After Dietary Supplementation: A Randomized Clinical Trial, J. Lipid Res. 41, 1376–1383.
Sanders, T.A.B. (1990) Essential Fatty Acid Requirements of Vegetarians in Pregnancy, Lactation, and Infancy, Am. J. Clin. Nutr. 70, 555S-559S.
Guesnet, P., Pugo-Gunsam, P., Maurage, C., Pinault, M., Giraudeau, B., Alessandri, J.M., Durand, G., Antoine, J.M., and Couet, C. (1999) Blood Lipid Concentrations of Docosahexaenoic and Arachidonic Acids at Birth Determine Their Relative Postnatal Changes in Term Infants Fed Breast-Milk or Formula, Am. J. Clin. Nutr. 70, 292–298.
Maurage, C., Guesnet, P., Pinault, M., de Lempdes, J.B.R., Durand, G., Antoine, J.M., and Couet, C. (1998) Effect of Two Types of Fish Oil Supplementation on Plasma and Erythrocyte Phospholipids in Formula-Fed Term Infants, Biol. Neonate 74, 416–429.
Makrides, M., Neumann, M., Simmer, K., Pater, J., and Gibson, R. (1995) Are Long-Chain Polyunsaturated Fatty Acids Essential Nutrients in Infancy? Lancet 345, 1463–1468.
Jørgensen, M.H., Hølmer, G., Lund, P., Hernell, O., and Michaelsen, K.F. (1998) Effect of Formula Supplemented with Docosahexaenoic Acid and Gamma-Linolenic Acid on Fatty Acid Status and Visual Acuity in Term Infants, J. Pediatr. Gastroenterol. Nutr. 26, 412–421.
Carlson, S.E. (1996) Arachidonic Acid Status of Human Infants: Influence of Gestational Age at Birth and Diets with Very Long-Chain n−3 and n−6 Fatty Acids, J. Nutr. 126, 1092S-1098S.
Møller, A., and Saxholt, E. (1996) Levnedsmiddeltabeller (The Composition of Foods), 4th edn., Levnedsmiddelstyrelsen, Søborg, Denmark.
Bligh, E.G., and Dyer, W.J. (1959) A Rapid Method of Total Lipid Extraction and Purification, Can. J. Biochem. Physiol. 37, 911–917.
Rose, H.G., and Oklander, M. (1965) Improved Procedure for the Extraction of Lipids from Human Erythrocytes, J. Lipid Res. 6, 428–431.
Sagredos, A.N. (1991) Fatty Acid Composition of Fish Oil Capsules, Fett Wiss. Technol.-Fat Sci. Technol. 93, 184–191.
Emken, E.A., Adlof, R.O., Hachey, D.L., Garza, C., Thomas, M.R., and Brown-Booth, L. (1989) Incorporation of Deuterium-Labeled Fatty Acids into Human Milk, Plasma, and Lipoprotein Phospholipids and Cholesteryl Esters, J. Lipid Res. 30, 395–402.
Demmelmair, H., Baumheuer, M., Koletzko, B., Dokoupil, K., and Kratl, G. (1998) Metabolism of U13C-Labeled Linoleic Acid in Lactating Women, J. Lipid Res. 39, 1389–1396.
Francois, C.A., Connor, S.L., Wander, R.C., and Connor, W.E. (1998) Acute Effects of Dietary Fatty Acids on the Fatty Acids of Human Milk, Am. J. Clin. Nutr. 67, 301–308.
Smit, E.N., Koopmann, M., Boersma, E.R., and Muskiet, F.A.J. (2000) Effect of Supplementation of Arachidonic Acid (AA) or a Combination of AA Plus Docosahexaenoic Acid on Breast-Milk Fatty Acid Composition, Prostaglandins Leukotrienes Essent. Fatty Acids 62, 335–340.
Francois, C.A., Connor, S.L., Bolewicz, L., and Connor, W.E. (2000) Changes in Breast-Milk and Plasma n−3 Fatty Acids After Supplementation with Flaxseed Oil, FASEB J. 14, A724 (Abstract).
Koletzko, B., Mrotzek, M., and Bremer, H.J. (1988) Fatty Acid Composition of Mature Human Milk in Germany, Am. J. Clin. Nutr. 47, 954–959.
Phinney, S.D., Odin, R.S., Johnson, S.B., and Holman, R.T. (1990) Reduced Arachidonate in Serum Phospholipids and Cholesteryl Esters Associated with Vegetarian Diets in Humans, Am. J. Clin. Nutr. 51, 385–392.
Mann, N.J., Johnson, L.G., Warrick, G.E., and Sinclair, A.J. (1995) The Arachidonic Acid Content of the Australian Diet Is Lower Than Previously Estimated, J. Nutr. 125, 2528–2535.
Jørgensen, M.H., Hernell, O., Lund, P., Hølmer, G., and Michaelsen, K.F. (1996) Visual Acuity and Erythrocyte Docosahexaenoic Acid Status in Breast-Fed and Formula-Fed Term Infants During the First Four Months of Life, Lipids 31, 99–105.
Olsen, S.F., Hansen, H.S., Sandström, B., and Jensen, B. (1995) Erythrocyte Levels Compared with Reported Dietary Intake of Marine n−3 Fatty Acids in Pregnant Women, Br. J. Nutr. 73, 387–395.
Prisco, D., Filippini, M., Francalanci, I., Paniccia, R., Gensini, G.F., Abbate, K., and Neri Serneri, G.G. (1996) Effect of n−3 Polyunsaturated Fatty Acid Intake on Phospholipid Fatty Acid Composition in Plasma and Erythrocytes, Am. J. Clin. Nutr. 63, 925–932.
Leaf, D.A., Connor, W.E., Barstad, L., and Sexton, G. (1995) Incorporation of Dietary n−3 Fatty Acids into the Fatty Acids of Human Adipose Tissue and Plasma Lipid Classes, Am. J. Clin. Nutr. 62, 68–73.
Tjønneland, A., Overvad, K., Thorling, E., and Ewertz, M. (1993) Adipose Tissue Fatty Acids as Biomarkers of Dietary Exposure in Danish Men and Women, Am. J. Clin. Nutr. 57, 629–633.
Marckmann, P., Lassen, A., Haraldsdottir, J., and Sandström, B. (1995) Biomarkers of Habitual Fish Intake in Adipose Tissue, Am. J. Clin. Nutr. 62, 956–959.
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Lauritzen, L., Jørgensen, M.H., Hansen, H.S. et al. Fluctuations in human milk long-chain PUFA levels in relation to dietary fish intake. Lipids 37, 237–244 (2002). https://doi.org/10.1007/s11745-002-0886-2
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DOI: https://doi.org/10.1007/s11745-002-0886-2