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Optimization of Rhamnolipid Production by P. aeruginosa Isolate P6

  • Original Article
  • Published:
Journal of Surfactants and Detergents

Abstract

Rhamnolipids are interesting microbial surfactants having great industrial importance. However, the main obstacles towards an economic production of rhamnolipids are low productivity and high raw-material costs. Therefore, this study aimed at optimization of the culture media as well as culture conditions using response surface methodology for maximum rhamnolipid production by Pseudomonas aeruginosa isolate P6, a promising rhamnolipid-producing isolate. The optimum medium for maximum rhamnolipid production was found to be a mineral salts medium with glycerol 2 % v/v as the carbon source. The optimum cultivation conditions using response surface methodology were found to be an incubation temperature of 30 °C, an agitation rate of 250 rpm, an inoculum size of 5 % v/v and unlike most studies, an initial pH of 7.5. The resulting model predicted data points that corresponded well to the experimental values. Optimization resulted in a threefold increase in rhamnolipid production reaching 7.54 g/L. The data are potentially useful for further industrial exploitation of rhamnolipid production by the studied isolate.

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Abbreviations

MSM:

Mineral salts media

RSM:

Response surface methodology

RL:

Rhamnolipids

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Faculty of Pharmacy, Ain shams University, Al Khalifa Al Maamoun St., Abbassia, Cairo, Egypt

    Ghadir Saeed El-Housseiny, Mohammad Mabrouk Aboulwafa, Khaled Anwer Aboshanab & Nadia Abdel Haleim Hassouna

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  1. Ghadir Saeed El-Housseiny
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  2. Mohammad Mabrouk Aboulwafa
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  3. Khaled Anwer Aboshanab
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  4. Nadia Abdel Haleim Hassouna
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Correspondence to Mohammad Mabrouk Aboulwafa.

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El-Housseiny, G.S., Aboulwafa, M.M., Aboshanab, K.A. et al. Optimization of Rhamnolipid Production by P. aeruginosa Isolate P6. J Surfact Deterg 19, 943–955 (2016). https://doi.org/10.1007/s11743-016-1845-4

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  • DOI: https://doi.org/10.1007/s11743-016-1845-4

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