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Hard-Surface Cleaning Using Lipases: Enzyme–Surfactant Interactions and Washing Tests

  • Original Article
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Journal of Surfactants and Detergents

Abstract

A commercial lipase (E.C. 3.1.1.3) from Thermomyces lanuginosus was studied in order to assess its interaction with commercial nonionic (Findet® 1214N/16, Findet 1214N/23 and Glucopon® 650) and anionic (linear alkylbenzene sulphonate; LAS) surfactants, as well as the cleaning action exerted by the enzyme on hard surfaces. Nonionic surfactants seem to prevent or delay enzyme penetration at the interface, thereby decreasing lipase activity. Notably, no inhibitory effect of the anionic surfactant LAS on lipase action was found, higher conversions being achieved after 20 min of enzymatic hydrolysis in the presence of this surfactant than in its absence. A device for testing detersive performance, the so-called bath–substrate–flow, was used in washing experiments with the lipase at different temperatures with or without surfactant. Employing two different oily stains (tributyrin and triolein), it was found that the lipase by itself increases detergency significantly, preventing the subsequent redeposition of the removed dirt. Expressions relating detersive efficiency to lipase concentration and temperature were obtained using "Statistical Design of Experiments" methodology.

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Acknowledgments

The “Ministerio de Educación y Ciencia” (Spain) supported the projects 1FD97-0931 and PB1998-1293, and awarded study grants to M.G.R. The “Comissão de Aperfeiçoamento de Pessoal de Nível Superior” (Brazil) awarded study grants to D.A.V.

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Authors and Affiliations

  1. Departamento de Ingeniería Química, Facultad de Ciencias, Universidad de Granada, Avda. Fuentenueva s/n, 18071, Granada, Spain

    Encarnación Jurado, Vicente Bravo, Germán Luzón, Mercedes Fernández-Serrano, Miguel García-Román, Deisi Altmajer-Vaz & José María Vicaria

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  1. Encarnación Jurado
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  2. Vicente Bravo
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  3. Germán Luzón
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  4. Mercedes Fernández-Serrano
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  5. Miguel García-Román
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  6. Deisi Altmajer-Vaz
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  7. José María Vicaria
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Correspondence to Encarnación Jurado.

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Jurado, E., Bravo, V., Luzón, G. et al. Hard-Surface Cleaning Using Lipases: Enzyme–Surfactant Interactions and Washing Tests. J Surfact Deterg 10, 61–70 (2007). https://doi.org/10.1007/s11743-006-1009-z

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  • DOI: https://doi.org/10.1007/s11743-006-1009-z

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