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An orthogonal test of the effect of NO3−, PO43−, K+, and Ca2+ on the growth and ion absorption of Elaeagnus angustifolia L. seedlings under salt stress

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Abstract

Excess salts in soil severely inhibit plant growth and yield. Various nutrients can relieve the inhibitory effect of salt on plants. Elaeagnus angustifolia is a tree species that is utilized in landscaping and ecological restoration in China and shows tolerance to saline soils. In this experiment, we first determined the optimal concentration of single-nutrient inputs for promoting growth of E. angustifolia seedlings and alleviating salt stress. The optimal nitrogen (NO3−), phosphorus (PO43−), potassium (K+), and calcium (Ca2+) concentrations under salt stress were 2, 0.6, 2, and 2 mM, respectively. Based on this result, we carried out an optimum nutrient ratio experiment by a four-factor and three-level orthogonal experiment. The results showed that under normal growth conditions, the optimal nutrient combinations for NO3−, PO43−, K+, and Ca2+ were 2, 0.6, 5, and 1 mM; 5, 0.2, 5, and 2 mM; and 5, 0.6, 1, and 5 mM, respectively, which increased plant height, biomass, and the chlorophyll content of E. angustifolia seedlings. Under salt stress, the optimal nutrient combinations were 5, 0.2, 5, and 2 mM; and 5, 0.6, 1, and 5 mM, respectively. This study provides a theoretical basis for optimal fertilization of E. angustifolia seedlings in saline-alkali soils.

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Acknowledgements

This work was supported by the major projects of science and technology in Shandong province (2017CXGC0311, 2017CXGC0313) and the Program for Scientific Research Innovation Team in Colleges and Universities of Shandong Province.

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Author notes

  1. Lingxiao Li and Tingting Zhu contributed equally to this work.

Authors and Affiliations

  1. Shandong Provincial Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, No. 88, East Wenhua Road, Jinan, 250014, Shandong, People’s Republic of China

    Lingxiao Li, Tingting Zhu, Jing Liu, Chen Zhao, Lingyu Li & Min Chen

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  1. Lingxiao Li
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  2. Tingting Zhu
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  3. Jing Liu
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  4. Chen Zhao
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  5. Lingyu Li
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Correspondence to Min Chen.

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Li, L., Zhu, T., Liu, J. et al. An orthogonal test of the effect of NO3−, PO43−, K+, and Ca2+ on the growth and ion absorption of Elaeagnus angustifolia L. seedlings under salt stress. Acta Physiol Plant 41, 179 (2019). https://doi.org/10.1007/s11738-019-2969-8

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