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In vitro selection of vanilla plants resistant to Fusarium oxysporum f. sp. vanillae

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Abstract

Fusarium oxysporum f. sp. vanillae (Fov), the most devastating pathogen of Vanilla planifolia, an important orchid used in the food industry, causes stem and root rot. Vanilla genotypes that are resistant to Fov currently do not exist, so vanilla plants that are resistant to this fungus are needed. In vitro selection offers an effective means to accomplish this objective. Resistant shoots were selected on Murashige and Skoog medium (MS) containing 8.88 µM of 6-benzyladenine and various concentrations of Fov culture filtrate (30, 40 and 50%, v/v). The control treatment (0%) was exclusively MS medium without any culture filtrate. After two 60-day selection cycles, 40 resistant shoots were obtained. In vitro, about 35% of the shoots were resistant to 50% Fov culture filtrate, while in vivo tests indicated that 26.6% of the plants had acquired resistance to the pathogen under greenhouse conditions after 9 weeks. The protocol employed in this study, which forms part of a wider genetic improvement program for this orchid, allowed Fov-resistant V. planifolia plants to be obtained within 420 days.

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Acknowledgements

The authors thank the “Programa para el Desarrollo Profesional Docente (PRODEP)” for financial support provided for the project “Biotechnological Basis for the Genetic Improvement of Vanilla planifolia” within the “Conservation, Management and Plant Breeding Network”. MARM thanks the Consejo Nacional de Ciencia y Tecnología (CONACyT) for the grant scholarship No. 275736, which allowed this work to be performed.

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Authors and Affiliations

  1. Laboratorio de Cultivo de Tejidos Vegetales, Colegio de Postgraduados Campus Córdoba, Km. 348 de la Carretera Federal Córdoba-Veracruz, Congregación Manuel León, Amatlán de los Reyes, Veracruz, Mexico

    Marco A. Ramírez-Mosqueda, Otto R. Leyva-Ovalle & Joaquín Murguía-González

  2. Laboratorio de Micropropagación Vegetal, Facultad de Ciencias Biológicas y Agropecuarias Región Córdoba-Orizaba, Universidad Veracruzana, Amatlan de los Reyes, 94945, Veracruz, Mexico

    Marco A. Ramírez-Mosqueda

  3. Laboratorio de Cultivo de Tejidos Vegetales, Instituto de Biotecnología y Ecología Aplicada (INBIOTECA), Universidad Veracruzana, Av. de las Culturas Veracruzanas No. 101, Campus para la Cultura, las Artes y el Deporte, Col. Emiliano Zapata, 91090, Xalapa, Veracruz, Mexico

    Lourdes G. Iglesias-Andreu, Juan C. Noa-Carrazana & José R. Bautista-Aguilar

  4. P.O. Box 7, Miki-cho post office, Ikenobe, 3011-2, Kagawa-ken, 761-0799, Japan

    Jaime A. Teixeira da Silva

  5. Laboratorio de Genética e Interacciones Planta Microorganismos, Facultad de Ciencias Agrícolas, Universidad Veracruzana, Circuito Gonzalo Aguirre Beltrán s/n, Zona Universitaria, 91000, Xalapa, Veracruz, Mexico

    Mauricio Luna-Rodríguez

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  1. Marco A. Ramírez-Mosqueda
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  2. Lourdes G. Iglesias-Andreu
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  3. Jaime A. Teixeira da Silva
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  4. Mauricio Luna-Rodríguez
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  5. Juan C. Noa-Carrazana
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  6. José R. Bautista-Aguilar
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  7. Otto R. Leyva-Ovalle
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  8. Joaquín Murguía-González
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Correspondence to Marco A. Ramírez-Mosqueda or Lourdes G. Iglesias-Andreu.

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Communicated by E. Kuzniak-Gebarowska.

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Ramírez-Mosqueda, M.A., Iglesias-Andreu, L.G., Teixeira da Silva, J.A. et al. In vitro selection of vanilla plants resistant to Fusarium oxysporum f. sp. vanillae. Acta Physiol Plant 41, 40 (2019). https://doi.org/10.1007/s11738-019-2832-y

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  • DOI: https://doi.org/10.1007/s11738-019-2832-y

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