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Toxicity of biosynthetic silver nanoparticles on the growth, cell ultrastructure and physiological activities of barley plant

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Abstract

Silver nanoparticles (AgNPs) were biosynthesized using the cell-free filtrate of bacterium Proteus mirabilis, reacted with 1 mM of AgNO3 solutions at 37 °C. The synthesis of AgNPs was monitored by UV–Vis spectroscopy and transmission electron microscopy (TEM) equipped with selected area electron diffraction (SAED). The results point to formation of spherical to cubical particles of AgNPs ranging in size from 5 to 35 nm with an average of 25 nm in diameter. The toxicity of Ag on barley (Hordeum vulgare L. cv. Gustoe) that was subjected to Ag+ as AgNO3 and AgNPs was explored. The grain germination and seedling growth of barley decreased in the presence of 0.1 mM Ag+ and was inhibited at 1 mM Ag+. In contrast, our results indicated that the AgNPs at low concentration (0.1 mM) could be useful for barley grain germination and seedling growth. However, the higher concentrations of AgNPs (0.5 and 1 mM) reduced grain germination and exhibited a stronger reduction in the root length. A decline in the photosynthetic pigments and disorganization of chloroplast grana thylakoids in Ag+ and AgNPs-treated plants confirmed the leaf chlorosis. An increase of plastoglobuli within chloroplasts was observed in Ag+ and AgNPs-treated leaves. Ag+ caused dense aggregation of nuclear chromatin materials and degeneration of mitochondria. Ag+ and AgNPs increased contents of malondialdehyde, soluble proteins, total phenolic compounds and activity of guaiacol peroxidase in barley leaves; these results point to activation of plant defence mechanisms against oxidative stress in barley.

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Abbreviations

Chl:

Chlorophyll

MDA:

Malondialdehyde

NPs:

Nanoparticles

POX:

Peroxidase

ROS:

Reactive oxygen species

SAED:

Selected area electron diffraction

TBA:

Thiobarbituric acid

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Acknowledgements

The project was funded by the University Research Projects (1-434-2623), Taif University, Saudi Arabia.

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Authors and Affiliations

  1. Biology Department, Faculty of Science, Taif University, Al-Haweiah, P.O. Box 888, 21974, Taif, Saudi Arabia

    K. A. Fayez, B. A. El-Deeb & N. Y. Mostafa

  2. Botany Department, Faculty of Science, Sohag University, Sohag, Egypt

    K. A. Fayez & B. A. El-Deeb

  3. Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, Egypt

    N. Y. Mostafa

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  1. K. A. Fayez
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  2. B. A. El-Deeb
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  3. N. Y. Mostafa
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Correspondence to K. A. Fayez.

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Communicated by A. Krolicka.

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Fayez, K.A., El-Deeb, B.A. & Mostafa, N.Y. Toxicity of biosynthetic silver nanoparticles on the growth, cell ultrastructure and physiological activities of barley plant. Acta Physiol Plant 39, 155 (2017). https://doi.org/10.1007/s11738-017-2452-3

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