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Identification for soybean host factors interacting with P3N-PIPO protein of Soybean mosaic virus

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Abstract

Previous evidence suggested the potyviral frame-shift protein P3N-PIPO is required for efficient viral intercellular movement. Host proteins are essential for virus to establish successful infection, many virus proteins play key roles in the viral infection cycle by interacting with that. Here, a yeast two-hybrid screening analysis was completed using Soybean mosaic virus (SMV) P3N-PIPO as the bait and a cDNA library from soybean infected with SMV as the prey to characterize the function of SMV P3N-PIPO. Fifty-four genes were isolated and analyzed by BLAST tools. Several genes encoding proteins that interacted with SMV P3N-PIPO were screened, including genes for inhibitory and transcription factors and those related to defense, transport, and photosynthesis. Some genes encoded proteins involved in metabolic activities in the chloroplast, such as photosystem I subunit PsaD and Calvin cycle protein CP12-2. Some genes were associated with defense responses, such as pathogenesis-related protein 1-like protein, stress-related protein-like protein, and stress enhanced protein 2. Other genes encoded defense-related transcription factors, such as WRKY transcription factor 51, while others were related to signal transduction, including a translationally-controlled tumor protein homolog, calcium-transporting ATPase 12, plasma membrane-type calcium-transporting ATPase 12-like protein, and calcineurin B-like protein 1-like isoform X1. Some genes coding for proteins related to affect viral plasmodesmata tracking, such as glucan endo-1,3-β-glucosidase (acidic isoform GL153). This study is the first to preliminarily delineate the interactions between SMV P3N-PIPO and host proteins related to defense responses during SMV infection.

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Abbreviations

ACA:

Calcium-transporting ATPase

AD:

Transcription-activating domain

Ade:

Adenine

BD:

DNA-binding domain

CBL:

Calcineurin B-like

CP:

Coat protein

CI:

Cylindrical inclusion

CYSB:

Cysteine proteinase inhibitor

EST:

Expressed sequence tag

GL153:

Glucan endo-1,3-β-glucosidase, acidic isoform GL153

His:

Histidine

JA:

Jasmonic acid

Leu:

Leucine

MP:

Movement protein

ORF:

Open reading frame

PD:

Plasmodesmata

PRB1:

Pathogenesis-related protein-1

PSAD:

Photosystem I subunit PsaD

SD:

Synthetic dropout medium

SMV:

Soybean mosaic virus

SNAP:

Soluble N-ethylmaleimide-sensitive fusion attachment protein

TCTP:

Translationally-controlled tumor protein

Trp:

Tryptophan

WRKY:

WRKY transcription factor

X-α-Gal:

5-Bromo-4-chloro-3-indolyl α-d-galactopyranoside

YPDA:

Yeast extract peptone dextrose medium supplemented with adenine hemisulfate

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Acknowledgments

We would like to thank Dr. Aiming Wang (Agriculture and Agri-Food Canada) for the gift of pPR3-N and pBT3-STE vectors. This work was supported by National Natural Science Foundation of China (31171574, 31371646, 31101411), the National Soybean Industrial Technology System of China (No. CARS-004) and Agricultural Science and Technology Independent Innovation Fund of Jiangsu Province (CX(11)4044).

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Authors and Affiliations

  1. National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China

    Puwen Song, Le Gao & Haijian Zhi

  2. Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu, China

    Xin Chen, Bingyue Wu & Xiaoyan Cui

Authors
  1. Puwen Song
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  2. Xin Chen
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  3. Bingyue Wu
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  4. Le Gao
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  5. Haijian Zhi
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  6. Xiaoyan Cui
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Correspondence to Haijian Zhi or Xiaoyan Cui.

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The authors declare that they have no conflict of interest.

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Communicated by O Ferrarese-Filho.

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Song, P., Chen, X., Wu, B. et al. Identification for soybean host factors interacting with P3N-PIPO protein of Soybean mosaic virus . Acta Physiol Plant 38, 131 (2016). https://doi.org/10.1007/s11738-016-2126-6

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  • DOI: https://doi.org/10.1007/s11738-016-2126-6

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