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The effect of natural and artificial fruit dehiscence on floss properties, seed germination and protein expression in Calotropis procera

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Abstract

A method was established, based on artificial fruit dehiscence, to collect seeds and silk fibers of C. procera without any loss. The seeds and floss obtained by this method were compared with those collected naturally. Natural fruit dehiscence caused a marked decrease in seeds and fibers yielded from C. procera. The seeds and fibers obtained under artificial fruit dehiscence were approximately sixfold higher than those obtained under natural fruit dehiscence. Furthermore, the application of artificial fruit dehiscence treatment had further advantages, such as a decrease in fiber diameter, and an increase in fiber density and cellulose content. However, this treatment had a slight negative impact on seed biomass and fiber birefringence. Fiber length, lignin and ash content of fibers were not affected by artificial fruit dehiscence. Seed germinability and seedling biomass were slightly decreased following application of artificial fruit dehiscence in comparison with natural fruit dehiscence. A significant difference was observed between the two treatments in amylase activity, soluble sugars and protein content. Some variation in protein expression was also apparent. Artificial fruit dehiscence lowered the expression of the short chain dehydrogenase, elongation factor 1-alpha, and peptidyl-prolyl cis–trans isomerase in comparison with natural dehiscence. Conversely, the expression of chitinase was enhanced in response to the artificial fruit dehiscence. Two conserved protein regions (prrl and vvet) were also observed.

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References

  • Abdul-Baki AA (1969) Metabolism of barley seed during early hours of germination. Plant Physiol 44:733–738

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Agu CV, Njoku OU, Chilaka FC, Okorie SA, Agbiogwu D (2012) Physico-chemical characterization of lignocellulosic fibre from Ampelocissus cavicaulis. IJBAS-IJENS 12:68–77

    Google Scholar 

  • Ahirwar JR (2012) Effect of seed size and weight on seed germination of Alangium lamarckii, Akola, India. Res J Recent Sci 1:320–322

    Google Scholar 

  • Albalasmeh AA, Berhe AA, Ghezzehei TA (2013) A new method for rapid determination of carbohydrate and total carbon concentrations using UV spectrophotometry. Carbohyd Polym 97:253–261

    Article  CAS  Google Scholar 

  • Barbosa MO, de Almeida-Cortez JS, da Silva SI, de Olivera AFM (2014) Seed oil content and fatty acid composition from different populations of Calotropis procera (Aiton) W.T Aiton (Apocynaceae). J Am Oil Cem Soc 91:1433–1441

    Article  CAS  Google Scholar 

  • Bradford MM (1976) A dye binding assay for protein. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Buysschaert G, Verstraete K, Savvides SN, Vergauwen B (2013) Structural and biochemical characterization of an atypical short-chain dehydrogenase/reductase reveals an unusual cofactor preference. FEBS J 280:1358–1370

    Article  CAS  PubMed  Google Scholar 

  • Chow PS, Landhäusser SM (2004) A method for routine measurements of total sugar and starch content in woody plant tissues. Tree Physiol 24(10):1129–1136

    Article  CAS  PubMed  Google Scholar 

  • de Morais GA, Takaki M (1998) Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. Carioca. Braz Arch Biol Techn 41:7–25

    Google Scholar 

  • Desai BB, Kotecha PM, Salunkhe DK (1997) Seeds handbook: biology, production, processing and storage. Marcel Dekker Inc., New York

    Google Scholar 

  • Ehrnrooth EML (1984) Change in pulp fiber density with acid-chlorite delignification. J Wood Chem and Techn 4:91–109

    Article  CAS  Google Scholar 

  • Erdman MD, Erdman BA (1981) Calotropis procera as a source of plant hydrocarbons. Econ Bot 35:467–472

    Article  CAS  Google Scholar 

  • Ferrándiz C (2002) Regulation of fruit dehiscence in Arabidopsis. J Exp Bot 53:2031–2038

    Article  PubMed  Google Scholar 

  • Frieden C (2015) ApoE: the role of conserved residues in defining function. Protein Sci 24:138–144

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Galal TM, Farahat EA, El-midany MM, Hassan LM (2015) Effect of temperature, salinity, light and time of dehiscence on seed germination and seedling morphology of Calotropis procera from urban habitats. Afr J Biotechn 14:1275–1282

    Article  Google Scholar 

  • Gamiely S, Smittle DA, Mills HA, Banna GI (1990) Onion weed size, weight, and elemental content affect germination and bulb yield. HortScience 25(5):522–523

    CAS  Google Scholar 

  • Gujjaiah S, Kumara C (2013) Evaluation of changes in α-amylase, β-amylase and protease during germination of cereals. Int J Agri Sci Res (IJASR) 3:55–62

    Google Scholar 

  • Hai A, Kizilbash NA, Alruwaili J (2013) Proteomics technology as an investigative tool in plant. Global Adv Res J Biotechn 2(2):013–016

    Google Scholar 

  • Hameed A, Gul A, Gulzar T (2014) Characterization of tomato germplasm through seed storage protein profiling by SDS-PAGE. Pak J Bot 46(3):827–832

    CAS  Google Scholar 

  • Hamza AA, Ghander AM, Sokkar TZN, Mabrouk MA, Ramadan WA (1995) On the determination of the refractive index of a fiber II. Graded index fiber. Pure Appl Opt 4:161–177

    Article  Google Scholar 

  • He L, Burris JS (1992) Respiration and carbohydrate metabolism during germination of sh2 and sh2 sweet corn seed. HortScience 27(12):1306–1308

    CAS  Google Scholar 

  • He M, Zhu C, Dong K, Zhang T, Cheng Z, Li J, Yan Y (2015). Comparative proteome analysis of embryo and endosperm reveals central differential expression proteins involved in wheat seed germination. BMC Plant Biol 15 (in press)

  • Hindi SZ (2013) Calotropis procera: the miracle shrub in the Arabian Peninsula. Int J Sci Eng Invest 2:48–57

    Google Scholar 

  • Ibrahim AH (2013a) Tolerance and avoidance responses to salinity and water stresses in Calotropis procera and Suaeda aegyptiaca. Turk J Agric For 37:352–360

    Google Scholar 

  • Ibrahim AH (2013b) Parental drought and defoliation effect on yield, grains biochemical aspects and drought performance of sorghum progeny. J Stress Physiol Biochem 9(1):258–272

    Google Scholar 

  • K’Opondo FBO (2011) Influence of drying method and fruit position on the mother plant on seed quality of spider plant (Cleome gynandra L.). Morphotypes from western Kenya. Adv Appl Sci Res 2(3):74–83

  • Kashem MA, Sultana N, Samanta SC, Kamal AMA (1995) Starch sugars, amylase and invertase activity in the germinating seeds of modern wheat varieties. J Nat Sci Coun Sri Lanka 23:55–61

    CAS  Google Scholar 

  • Kromina KA, Ignatov AN, Abdeeva IA (2008) Review: Role of peptidyl-prolylcis cis/trans-isomerases in pathologic processes. Biochem (Moscow) Suppl Ser A Membr Cell Biol 2:243–251

    Article  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227(5259):680–685

    Article  CAS  PubMed  Google Scholar 

  • Liogier HA (1995) Descriptive flora of Puerto Rico and adjacent islands. Editorial de la Universidad de Puerto Rico, San Juan, PR, vol 4, p 617

  • Maldini M, Natella F, Baima S, Morelli G, Scaccini C, Langridge J, Astarita G (2015) Untargeted metabolomics reveals predominant alterations in lipid metabolism following light exposure in Broccoli sprouts. Int J Mol Sci 16:13678–13691

    Article  PubMed Central  PubMed  Google Scholar 

  • Naguib MI (1964) Effect of sevin on the carbohydrate and nitrogen metabolism during the germination of cotton seeds. Ind J Exp Biol 2:149–152

    Google Scholar 

  • Nathan R, Katul G, Bohrer G, Kuparinen A, Soons MB, Thompson SE, Trakhtenbrot A, Horn HS (2011) Mechanistic models of seed dispersal by wind. Theor Ecol 4:113–132

    Article  Google Scholar 

  • Palapol Y, Kunyamee S, Thongkhumc M, Ketsac S, Fergusonf IB, van Doorng WG (2015) Expression of expansin genes in the pulp and the dehiscence zone of ripening durian (Durio Zibethinus) fruit. J Plant Physiol 182:33–39

    Article  CAS  PubMed  Google Scholar 

  • Payal C, Sharma RA (2015) An overview on giant milkweed (Calotropis procera (Ait.) Ait. f.). J Plant Sci 3:19–23

    Google Scholar 

  • Pejic BM, Kostic MM, Skundric PD, Petar D, Praskalo JZ (2008) The effects of hemicelluloses and lignin removal on water uptake behavior of hemp fibers. Bioresour Technol 99(15):7152–7159

    Article  CAS  PubMed  Google Scholar 

  • Perkins DN, Pappin DJ, Creasy DM, Cottrell JS (1999) Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis 20:3551–3567

    Article  CAS  PubMed  Google Scholar 

  • Raimi OG, Olaitan SN, Fajana OO, Sanni JO (2012) Effect of germination time on fat and protein contents, and α-amylase activity of Guinea Corn (Sorghum vulgare). Pak J Food Sci 22(2):86–89

    Google Scholar 

  • Rajeswari J, Ramakrishna RP (2002) Storage protein degradation in germinating horse gram seeds. Indian J Plant Physiol 7:314–320

    Google Scholar 

  • Riazi A, Matsuda K, Arslan A (1985) Water stress-induced changes in concentrations of proline and other solutes in growing regions of young barley leaves. J Exp Bot 36:1716–1725

    Article  CAS  Google Scholar 

  • Schmid FX, Frech C, Scholz C et al (1996) Catalyzed and assisted protein folding of ribonuclease T1. Biol Chem 377:417–424

    CAS  PubMed  Google Scholar 

  • Seagull RS, Oliveri V, Murphy K, Binder A, Kothari S (2000) Cotton fiber growth and development 2. Changes in cell diameter and wall birefringence. J Cotton Sci 4:97–104

    Google Scholar 

  • Sebastiani FL, Farrell LB, Vasquez M, Beachy RN (1991) Conserved amino acid sequences among plant proteins sorted to protein bodies and plant vacuoles. Can they play a role in protein sorting? Eur J Biochem 199:441–450

    Article  CAS  PubMed  Google Scholar 

  • Sharma S, Amritphale D (2008) Light environment in pre- and post-dehiscent fruits affects seed germination in Calotropis procera. Environ Exp Bot 62:45–53

    Article  Google Scholar 

  • Sokkar TZN, Ramadan WA, Shams El-Din MA, Wahba HH, Aboleneen SS (2014) Bent induced refractive index profile variation and mode field distribution of step-index multimode optical fiber. Opt Laser Eng 53:133–141

    Article  Google Scholar 

  • Sriyook S, Siriatiwat S, Siriphanich J (1994) Durian fruit dehiscence—water status and ethylene. HortScience 29(10):1195–1198

    CAS  Google Scholar 

  • Tian B, Xie B, Shi J, Wu J, Cai Y, Xu T, Xue S, Deng Q (2010) Physicochemical changes of oat seeds during germination. Food Chem 119:1195–1200

    Article  CAS  Google Scholar 

  • Tuntawiroon N, Samootsakorn P, Theeraraj G (1984) The environmental implications of the use of Calotropis gigantea as a textile fabric. Agr Ecosyst Environ 11:203–212

    Article  Google Scholar 

  • Ursin VM, Irvine JM, Hiatt WR, Shewmaker CK (1991) Developmental analysis of elongation Factor- Iα expression in transgenic tobacco. Plant Cell 3:583–591

    PubMed Central  CAS  PubMed  Google Scholar 

  • Varshney AC, Bhoi KL (1987) Some possible industrial properties of Calotropis procera (Aak) floss fiber. Biol Waste 22:157–161

    Article  CAS  Google Scholar 

  • Varshney AC, Bhoi KL (1988) Cloth from bast fibre of the Calotropis procera (Aak) plant. Biol Waste 26:229–232

    Article  CAS  Google Scholar 

  • Venkatesh GM, Dweltz NE (1976) Birefringence of native, alkali-swollen, and crosslinked cotton fibers. J Appl Polym Sci 20:273–277

    Article  CAS  Google Scholar 

  • Ververis C, Georghiou K, Danielidis D, Hatzinikolaou DG, Santas P, Santas R, Corleti V (2007) Cellulose, hemicelluloses, lignin and ash content of some organic materials and their suitability for use as paper pulp supplements. Bioresour Technol 98:296–301

    Article  CAS  PubMed  Google Scholar 

  • Wang Q, Zhang Y, Hou Y, Wang P, Zhou S, Ma X, Zhang N (2012) Purification, characterization of a CkChn134 protein from Cynanchum komarovii seeds and synergistic effect with CkTLP against Verticillium dahlia. Protein Sci 21:865–875

    Article  PubMed Central  PubMed  Google Scholar 

  • Wender NJ, Polisetty CR, Donohue K (2005). Density-dependent processes influencing the evolutionary dynamics of dispersal: A functional analysis of seed dispersal in Arabidopsis thaliana (Brassicaceae). Am J Bot 92(6):960–971

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Acknowledgments

We would like to express our sincere gratitude to Professor Robert C. Ford (Chair in Structural Biology, Faculty of Life Science, Manchester University) for giving access to the laboratory and research facilities. Also, our sincere thanks is extended to Eleanor Martin (a member of Prof. Robert C. Ford`s lab) for her contribution in proofreading.

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Authors and Affiliations

  1. Botany Department, Faculty of Science, Port Said University, Port Said, Egypt

    Ali Hassan Ibrahim

  2. Biology and Chemistry Department, University College at Al-Qunfudah, Umm AL-Qura University, Makkah, Saudi Arabia

    Ateeq Ahmed Al-Zahrani

  3. Physics Department, Faculty of Science, University of Damietta, New Damietta, Egypt

    Hamdy Hossein Wahba

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  1. Ali Hassan Ibrahim
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  2. Ateeq Ahmed Al-Zahrani
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Correspondence to Ali Hassan Ibrahim.

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Communicated by W. Wang.

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Ibrahim, A.H., Al-Zahrani, A.A. & Wahba, H.H. The effect of natural and artificial fruit dehiscence on floss properties, seed germination and protein expression in Calotropis procera . Acta Physiol Plant 38, 15 (2016). https://doi.org/10.1007/s11738-015-2033-2

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  • DOI: https://doi.org/10.1007/s11738-015-2033-2

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