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Transcriptome analysis of the genes related to the morphological changes of Paulownia tomentosa plantlets infected with phytoplasma

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Abstract

Paulownia witches’ broom (PaWB) caused by phytoplasma is a destructive disease of Paulownia in China and has been studied over the past 40 years. Recently, our research team found that methyl methanesulfonate (MMS) could help Paulownia plants recover from the symptoms of Paulownia witches’ broom in which the expression levels of genes related to photosynthesis, carbohydrate and nitrogen metabolism, plant-pathogen interaction, circadian rhythm changed. However, the molecular mechanisms underlying the interaction between PaWB and phytoplasma are still not fully understood. Here, high-throughput mRNA sequencing and de novo assembly were conducted in order to investigate gene expression profiles in three Paulownia tomentosa samples (healthy plantlets, diseased plantlets, and diseased plantlets treated with MMS. A total of 85,545 all-unigene were obtained, 2540 of which were significantly differentially expressed among the three samples. Some genes associated with folate and fatty acid synthesis, signal transduction pathways of plant hormones were identified, and their expression patterns were further validated using quantitative real-time PCR. To our knowledge, this is the first transcriptome-based study to demonstrate variations of gene expression related to morphological changes in metabolic pathways of phytoplasma-infected P. tomentosa plants. The information provided in this study will help understand the molecular pathogenesis of the Paulownia plant in response to phytoplasma infection.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (Grant No. 30271082, 30571496), by the Outstanding Talents Project of Henan Province (Grant No. 122101110700), by the Transformation Project of the National Agricultural Scientific and Technological Achievement of China (Grant No. 2012GB2D000271), and by Science and Technology Innovation Team Project of Zhengzhou City (Grant No. 121PCXTD515).

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Authors and Affiliations

  1. Institute of Paulownia, Henan Agricultural University, Zhengzhou, 450002, Henan, People’s Republic of China

    Guoqiang Fan, Xibing Cao, Zhenli Zhao & Minjie Deng

  2. College of Forestry, Henan Agricultural University, Zhengzhou, 450002, Henan, People’s Republic of China

    Guoqiang Fan, Xibing Cao, Zhenli Zhao & Minjie Deng

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  1. Guoqiang Fan
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  2. Xibing Cao
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Correspondence to Guoqiang Fan.

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Communicated by J.-H Liu.

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Fan, G., Cao, X., Zhao, Z. et al. Transcriptome analysis of the genes related to the morphological changes of Paulownia tomentosa plantlets infected with phytoplasma. Acta Physiol Plant 37, 202 (2015). https://doi.org/10.1007/s11738-015-1948-y

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