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Diversity among wild accessions of Bacopa monnieri (L.) Wettst. and their morphogenetic potential

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Abstract

Biochemical and molecular diversity among 14 accessions of Bacopa monnieri (L.) Wettst. collected from various locations of India was investigated. A significant variation was recorded in bacoside A contents of these accessions. A scatter plot of principle component analysis based on bacoside A contents clubbed these populations into two major groups and accession BM14 was placed separately. Similarly, about 35 % variations were detected in these populations based on combined data of random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR). Individually, ISSR markers detected higher variation (44.9 %) as compared to RAPD markers (23 %). Clustering based on molecular marker data grouped these accessions into two major groups and also placed accession BM14 as an out group. The shoot organogenic potential of leaf explants taken from microshoots and rooting of microshoots also varied among accessions. Maximum shoot organogenic potential was observed in accession BM5 and maximum rooting potential was observed in accessions BM1, BM2, BM7, BM10 and BM14. Present study is an important step for developing long-term strategy for conservation of this important medicinal herb.

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References

  • Aggarwal D, Kumar A, Reddy MS (2011) Agrobacterium tumefaciens mediated genetic transformation of selected elite clone(s) of Eucalyptus tereticornis. Acta Physiol Plant 33:1603–1611

    Article  CAS  Google Scholar 

  • Aggarwal D, Kumar A, Reddy MS (2012) Factors affecting genetic transformation and shoot organogenesis of Bacopa monnieri (L.) Wettst. J Plant Biochem Biotechnol 22:382–391

    Article  Google Scholar 

  • Anonymous (1988) Wealth of India, raw materials. Council of Scientific and Industrial Research (CSIR), New Delhi

    Google Scholar 

  • Barik S, Senapati SK, Aparajita S, Mohapatra A, Rout GR (2006) Identification and genetic variation among Hibiscus species (Malvaceae) using RAPD markers. Z Naturforsch 61:123–128

    CAS  Google Scholar 

  • Bornet B, Branchard M (2001) Nonanchored inter simple sequence repeat (ISSR) markers reproducible and specific tools for genome fingerprinting. Plant Mol Biol Rep 19:209–215

    Article  CAS  Google Scholar 

  • Ceasar SA, Maxwell SL, Parsad KB, Karithigan M, Ignacimuthu S (2010) Highly efficient shoot regeneration of Bacopa monnieri (L.) using a two-stage culture procedure and assessment of genetic integrity of micropropagated plants by RAPD. Acta Physiol Plant 32:443–452

    Article  CAS  Google Scholar 

  • Chatterjee SN, Pradeep AR (2003) Molecular markers (RAPD) associated with growth, yield and origin of the silkworm, Bombyx mori in India. Russ J Genet 39:1612–1624

    Article  CAS  Google Scholar 

  • Cirak C, Radusiene J, Karabuk B, Janulis V (2007) Variation of bioactive substances and morphological traits in Hypericum perforatum populations from Northern Turkey. Biochem Sys Ecol 35:403–409

    Article  CAS  Google Scholar 

  • Cirak C, Radusiene J, Stanius Z, Camas N, Caliskan O, Odabas MS (2012) Secondary metabolites of Hypericum orientale L. growing in Turkey: variation among populations and plant parts. Acta Physiol Plant 34:1313–1320

    Article  CAS  Google Scholar 

  • Darokar MP, Suman PSK, Shasany AK, Kumar S (2001) Low levels of genetic diversity detected by RAPD analysis in geographically distinct accessions of Bacopa monnieri. Genet Resour Crop Evol 48:555–558

    Article  Google Scholar 

  • Dick JMcP, Bisset H, McBeath C (1996) Provenance variation in rooting ability of Calliandra calothyrsus. For Ecol Manag 87:175–184

    Article  Google Scholar 

  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissues. Focus 12:13–15

    Google Scholar 

  • Fernie AR (2007) The future of metabolic phytochemistry: larger numbers of metabolites, higher resolution, greater understanding. Phytochemistry 68:2861–2880

    Article  CAS  PubMed  Google Scholar 

  • Gajera BB, Kumar N, Singh AS, Punvar BS, Ravikiran R, Subhash N, Jadeja GC (2010) Assessment of genetic diversity in castor (Ricinus communis L.) using RAPD and ISSR markers. Ind Crops Prod 32:491–498

    Article  CAS  Google Scholar 

  • Govindrajan R, Vijaykumar M, Pushpangadan P (2005) Antioxidant approach to disease management and the role of rasayana herbs of ayurveda. J Ethnopharmacol 19(2):165–178

    Article  Google Scholar 

  • Guo CY, Cao ZY (1982) Effect of different genotypes on induction frequency in anther and scutellum culture of maize in vitro. Heredity 4(4):8–10

    Google Scholar 

  • Hsia C, Korban SS (1996) Organogenesis and somatic embryogenesis in callus cultures of Rosa hybrida and Rosa chinensis minima. Plant Cell Tissue Org Cult 44:1–6

    Article  CAS  Google Scholar 

  • Islam A (2004) Genetic diversity of the genus Curcuma in Bangladesh and further biotechnological approaches for in vitro regeneration and long-term conservation of C. longa germplasm. PhD. thesis, University of Hannover

  • Jayanthi M, Mandal PK (2001) Plant regeneration through somatic embryogenesis and RAPD analysis of regenerated plants in Tylophora indica (Burm. f. Merrill). In Vitro Cell Dev Biol Plant 37:576–580

    Article  CAS  Google Scholar 

  • Karthikeyan A, Madhanraj A, Pandian SK, Ramesh M (2011) Genetic variation among highly endangered Bacopa monnieri (L.) Pennell from Southern India as detected using RAPD analysis. Genet Resour Crop Evol 58:769–782

    Article  Google Scholar 

  • Khanna HK, Raina SK (1998) Genotype x culture media interaction effects on regeneration response of three Indica rice cultivars. Plant Cell Tissue Org Cult 52:145–153

    Article  CAS  Google Scholar 

  • Lim W, Mudge KW, Vermeylen F (2005) Effects of population, age, and cultivation methods on ginsenoside content of wild American ginseng (Panax quinquefolium). J Agric Food Chem 53:8498–8505

    Article  CAS  PubMed  Google Scholar 

  • Mohapatra A, Rout GR (2005) Identification and genetic variation among rose cultivars. Z Naturforsch 60:611–617

    CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nadeem M, Rikhari HC, Kumar A, Palni LMS, Nandi SK (2002) Taxol content in the bark of Himalayan Yew in relation to tree age and sex. Phytochemistry 60:627–631

    Article  CAS  PubMed  Google Scholar 

  • Nadeem M, Palni LMS, Kumar A (2007) Podophyllotoxin content, above- and below ground biomass in relation to altitude in P. hexandrum populations from Kumaun region of the Indian Central Himalaya. Planta Med 73:388–391

    Article  CAS  PubMed  Google Scholar 

  • Nadkarni KM (1976) Indian Materia Medica. Popular Prakashan Private, Bombay, p 968

    Google Scholar 

  • National Medicinal Plants Board (2004) 32 prioritized medicinal plants, National Informatics Centre, Ministry of Health and Family Welfare, Department of Ayush, Government of India. http://www.nmpb.nic.in/sarpgandha.htm

  • National Medicinal Plants Board (NMPB) Technology Information Forecasting and Assessment Council (TIFAC), Department of Science and Technology, Government of India (2007). http://www.nmpb.nic.in/prioritisedmedicinalplants.htm

  • National Medicinal Plants Board, NMPB (2008) Agrotechniques of selected medicinal plants. National Medicinal Plants Board, Department of Ayush, Ministry of Health and Family Welfare, Govt of India. TERI Press, New Delhi, pp 33–38

    Google Scholar 

  • Nayak S, Naik PK, Acharya LK, Pattnaik AK (2006) Detection and evaluation of genetic variation in 17 promising cultivars of turmeric (Curcuma longa L.) using 4C nuclear DNA content and RAPD markers. Cytologia 71(1):49–55

    Article  CAS  Google Scholar 

  • Pandey SK, Ramesh B, Gupta PK (1994) Study on effect of genotype and culture medium on callus formation and plant regeneration in rice (Oryza sativa L.) Indian. J Genet 54:293–299

    Google Scholar 

  • Pharmawati M, Yen G, McFarlane IJ (2004) Application of RAPD and ISSR markers to analyse molecular relationships in Grevillea (Proteaceae). Aust Syst Bot 17:49–61

    Article  CAS  Google Scholar 

  • Phrompittayarat W, Putalun W, Tanaka H, Jetiyanon K, Wittaya-areekuf S, Ingkaninan K (2007) Comparison of various extraction methods of Bacopa monnieri. Naresuan Univ J 15(1):29–34

    Google Scholar 

  • Prasad R, Bagde US, Puspangadan P, Varma A (2008) Bacopa monnieri L. pharmacological aspects and case studies involving Piriformospora indica. Int J Integr Biol 3:100–110

    CAS  Google Scholar 

  • Rajani M (2008) Bacopa monnieri, a nootropic drug. In: Ramawat KG, Merillon JM (eds) Bioactive molecules and medicinal plants. Springer, Berlin, pp 175–195

    Chapter  Google Scholar 

  • Ramesh M, Vijaya Kumar K, Karthikeyan A, Pandian SK (2011) RAPD based genetic stability analysis among micropropagated, synthetic seed derived and hardened plants of Bacopa monnieri (L.): a threatened indian medicinal herb. Acta Physiol Plant 33:163–171

    Article  Google Scholar 

  • Rastogi S, Pal R, Kulshreshtha DK (1994) Bacoside A3—a triterpenoid saponin from Bacopa monnieri. Phytochemistry 36:13–137

    Article  Google Scholar 

  • Rout GR, Das G (2002) An assessment of genetic integrity of micropropagated plants of Plumbago zeylanica by RAPD markers. Biol Plant 45:27–32

    Article  CAS  Google Scholar 

  • Russo A, Borrelli F (2005) Bacopa monniera, a reputed nootropic plant: an overview. Phytomedicine 12(4):305–317

    Article  CAS  PubMed  Google Scholar 

  • Sanaa A, Boulilab A, Bejaouia A, Boussaida M, Fadhela N (2012) Variation of the chemical composition of floral volatiles in the endangered Tunisian Pancratium maritimum L. populations (Amaryllidaceae). Ind Crops Prod 40:312–317

    Article  CAS  Google Scholar 

  • Sharma N, Satsangi R, Pandey R, Singh R, Kaushik N, Tyagi RK (2012) In vitro conservation of Bacopa monnieri (L.) using mineral oil. Plant Cell Tissue Org Cult. doi:10.1007/s11240-012-0194-x

    Google Scholar 

  • Shrivastava N, Rajani M (1999) Multiple shoot regeneration and tissue culture studies on Bacopa monnieri (L.) Pennell. Plant Cell Rep 18:919–923

    Article  CAS  Google Scholar 

  • Singh HK, Dhawan BN (1997) Neurophychopharmacological effects of the Ayurvedic nootropic Bacopa monniera Linn. (Brahmi). Indian J Pharmacol 29:8359–8365

    Google Scholar 

  • Tiwari V, Singh BD, Tiwari NK (1998) Shoot regeneration and somatic embryogenesis from different explants of Brahmi Bacopa monniera (L.) Wettst. Plant Cell Rep 17:538–543

    Article  CAS  Google Scholar 

  • Tiwari V, Tewari KN, Singh BD (2001) Comparative studies of cytokinins on in vitro propagation of Bacopa monniera. Plant Cell Tissue Org Cult 66:9–16

    Article  CAS  Google Scholar 

  • Tiwari V, Tewari KN, Singh BD (2006) Shoot bud regeneration from different explants of Bacopa monniera (L). Wettst. by trimethoprim and bavistin. Plant Cell Rep 25:629–635

    Article  CAS  PubMed  Google Scholar 

  • Tripathi N, Chouhan DS, Saini N, Tiwari S (2012) Assessment of genetic variations among highly endangered medicinal plant Bacopa monnieri (L.) from Central India using RAPD and ISSR analysis. 3 Biotech 2:327–336

    Article  PubMed Central  Google Scholar 

  • Vahdati K, Leslie C, Zamani Z, McGranahan G (2004) Rooting and acclimatization of in vitro grown shoots from mature trees of three persian walnut cultivars. HortScience 39(2):324–327

    Google Scholar 

  • Williams JG, Kubelik AR, Livak KJ, Rafaleski JA, Tiongey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535

    Article  CAS  PubMed Central  PubMed  Google Scholar 

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Acknowledgments

Authors are thankful to University Grant Commission (UGC), Govt. of India, New Delhi for the financial assistance. Prof. R.K. Sharma, Thapar University is thanked for his help in PCA analysis. Thanks are also due to TIFAC-CORE, Thapar University Patiala for the facilities to carry out this work.

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  1. Department of Biotechnology, TIFAC-Center of Relevance and Excellence in Agro and Industrial Biotechnology (CORE), Thapar University, Patiala, 147004, India

    Mahima Bansal, Anil Kumar & M. Sudhakara Reddy

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  1. Mahima Bansal
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  2. Anil Kumar
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  3. M. Sudhakara Reddy
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Correspondence to Anil Kumar.

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Communicated by M. Capuana.

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Bansal, M., Kumar, A. & Sudhakara Reddy, M. Diversity among wild accessions of Bacopa monnieri (L.) Wettst. and their morphogenetic potential. Acta Physiol Plant 36, 1177–1186 (2014). https://doi.org/10.1007/s11738-014-1493-0

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